Skip to main content

Advertisement

SpringerLink
Log in

A novel subgenotype 3a hepatitis E virus isolated from pigs in China

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Hepatitis E virus (HEV) infection is an emerging disease with zoonotic transmission that represents a serious public health concern, especially in developing countries. Here we characterize a novel HEV strain CCST-517, which possesses a complete genome sequence of 7284 bp with typical HEV genome organization including 5′ and 3′ non-coding regions and three open reading frames. The sequence identities of CCST-517 with known HEV genotype 1, 2, 3, and 4 were 73.4–73.7, 73.2, 80.4–90.4, and 75.1–75.7%, respectively. Phylogenetic analysis clustered CCST-517 to the clade of HEV genotype 3a, together with the Japanese human HEV isolate (HE-JA10) and United States human HEV isolate (HEV-US2). Similarity plot analysis indicated that the fragment extending from 4500 to 5500 nt included evidence of one intra-genotype recombination event in the genome sequence of the CCST-517 strain. To our knowledge, this is the first report of HEV genotype 3a with its complete genome sequence revealed in China. Our findings revealed a close phylogenetic relationship of CCST-517 to human HEV-US2 and HE-JA10, implying cross-species transmission of HEV between pigs and humans.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Y.J. Song, W.J. Park, B.J. Park, J.B. Lee, S.Y. Park, C.S. Song, N.H. Lee, K.H. Seo, Y.S. Kang, I.S. Choi, Clin. Exp. Vaccine Res. 3, 29–36 (2014). doi:10.7774/cevr.2014.3.1.29

    Article  CAS  PubMed  Google Scholar 

  2. G.R. Reyes, M.A. Purdy, J.P. Kim, K.C. Luk, L.M. Young, K.E. Fry, D.W. Bradley, Science 247, 1335–1339 (1990). doi:10.1126/science.2107574

    Article  CAS  PubMed  Google Scholar 

  3. P. Gallian, Y. Piquet, A. Assal, R. Djoudi, J. Chiaroni, J. Izopet, P. Tiberghien, Transfus. Clin. Biol. 21, 173–177 (2014). doi:10.1016/j.tracli.2014.07.007

    Article  CAS  PubMed  Google Scholar 

  4. M.G. Martinez Wassaf, M.B. Pisano, P.A. Barril, O.C. Elbarcha, M.A. Pinto, J. Mendes de Oliveira, P. DiGiusto, S.V. Nates, V.E. Re, J. Clin. Microbiol. 61, 334–339 (2014). doi:10.1016/j.jcv.2014.08.016

    Google Scholar 

  5. A.W. Tam, M.M. Smith, M.E. Guerra, C.C. Huang, D.W. Bradley, K.E. Fry, G.R. Reyes, Virol. 185, 120–131 (1991). doi:10.1016/0042-6822(91)90760-9

    Article  CAS  Google Scholar 

  6. H. Jin, Y. Zhao, X. Zhang, B. Wang, P. Liu, Epidemiol. Infect. 144, 2098–2106 (2016). doi:10.1017/S0950268816000418

    Article  CAS  PubMed  Google Scholar 

  7. S.A. Chaudhry, N. Verma, G. Koren, Can. Fam. Physician 61, 607–608 (2015)

    PubMed  PubMed Central  Google Scholar 

  8. I. Ahmad, R.P. Holla, S. Jameel, Virus Res. 161, 47–58 (2011). doi:10.1016/j.virusres.2011.02.011

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. P. Liu, L. Li, L. Wang, Q. Bu, H. Fu, J. Han, Y. Zhu, F. Lu, H. Zhuang, Genet. Evol. 12, 428–434 (2012). doi:10.1016/j.meegid.2012.01.017

    Article  CAS  Google Scholar 

  10. H. Tian, X. Fu, W. Li, Y. Huang, J. Sun, G. Zhou, C. Zhou, Q. Shen, S. Yang, W. Zhang, Hepat Mon. 15, e25367 (2015). doi:10.5812/hepatmon.25367v2

    Article  PubMed  PubMed Central  Google Scholar 

  11. H. Ning, Z. Niu, R. Yu, P. Zhang, S. Dong, Z. Li, Vet. Microbiol. 121, 125–130 (2007). doi:10.1016/j.vetmic.2006.11.006

    Article  CAS  PubMed  Google Scholar 

  12. Y. Geng, C. Zhao, J. Fan, T.J. Harrison, H. Zhang, H. Lian, K. Geng, Y. Wang, Genet. Evol. 20, 413–417 (2013). doi:10.1016/j.meegid.2013.10.003

    Article  Google Scholar 

  13. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. W. Zhang, Y. He, H. Wang, Q. Shen, L. Cui, X. Wang, S. Shao, X. Hua, Emerg. Infect. Dis. 16, 1630–1632 (2010). doi:10.3201/eid1610.100873

    Article  PubMed  PubMed Central  Google Scholar 

  15. W. Zhang, S. Yang, Q. Shen, F. Huang, T. Shan, Z. Yang, L. Cui, J. Zhu, X. Hua, Vet. Microbiol. 140, 193–195 (2010). doi:10.1016/j.vetmic.2009.06.037

    Article  CAS  PubMed  Google Scholar 

  16. H. Tokita, H. Harada, Y. Gotanda, M. Takahashi, T. Nishizawa, H. Okamoto, J. Gen. Virol. 84, 421–427 (2003). doi:10.1099/vir.0.18802-0

    Article  CAS  PubMed  Google Scholar 

  17. G.G. Schlauder, G.J. Dawson, J.C. Erker, P.Y. Kwo, M.F. Knigge, D.L. Smalley, J.E. Rosenblatt, S.M. Desai, I.K. Mushahwar, J. Gen. Virol. 79, 447–456 (1998). doi:10.1099/0022-1317-79-3-447

    Article  CAS  PubMed  Google Scholar 

  18. J.C. Erker, S.M. Desai, G.G. Schlauder, G.J. Dawson, I.K. Mushahwar, J. Gen. Virol. 80, 681–690 (1999). doi:10.1099/0022-1317-80-3-681

    Article  CAS  PubMed  Google Scholar 

  19. E. Simon-Loriere, E.C. Holmes, Nat. Rev. Microbiol. 9, 617–626 (2011). doi:10.1038/nrmicro2614

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. H. van Cuyck, J. Fan, D.L. Robertson, P. Roques, J. Virol. 79, 9306–9314 (2005). doi:10.1128/JVI.79.14.9306-9314.2005

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This study is supported by grants from ChinaScience and Technology Major Project “Prevention and Control of Major Animal and Poultry Epidemics and Comprehensive Technology for Efficiently Safeguarding the Livestock Development” (2016YFD0500904), National 863 Program-National High Technology Research and Development Program (2012AA02A401), and Jilin Province Science and Technology Development Projects (20130204014YY).

Author contributions

XPW and ZHL conceived and designed the study; YFC, XPW, and LSZ contributed to the writing and revision of the manuscript; YFC, CMG, and CBS performed laboratory testing; DL and YY contributed to the genome sequencing and analysis.

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China

    Yufeng Cao, Lisai Zhu, Dan Liu, Changming Guo, Yue Yuan & Xinping Wang

  2. Changchun Institute of Biological Products Co. Ltd., 3456 Xian Road, Changchun, 130062, Jilin, China

    Yufeng Cao, Chengbo Shi & Zhaohui Liu

  3. Key Laboratory for Zoonosis, Ministry of Education, Institute for Zoonosis of Jilin University, Changchun, 130062, China

    Xinping Wang

Authors
  1. Yufeng Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lisai Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Changming Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yue Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chengbo Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhaohui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xinping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Zhaohui Liu or Xinping Wang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

Edited by William Dundon.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Fig. 1

Intra-genotype recombination event revealed in the CCST-517 strain. Recombination analysis was performed by SimPlot software. Bootscan analysis revealed one intra-genotype recombination event within the sequence of the CCST-517 strain, when HEV-US2, HE-JA10, JIO-swJ19-1 and ZhJ-PJ050-3 strains used as reference sequence, and CCST-517 strain as query sequence (a). Two breakpoints (4,675nt and 5,075nt) were detected in the CCST-517 genome, generating three recombination fragments (b) (TIFF 1157 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cao, Y., Zhu, L., Liu, D. et al. A novel subgenotype 3a hepatitis E virus isolated from pigs in China. Virus Genes 53, 483–486 (2017). https://doi.org/10.1007/s11262-017-1435-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-017-1435-1

Keywords

Search

Navigation