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Full-length infectious clone of an Iranian isolate of chicken anemia virus

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Abstract

An Iranian field strain of chicken anemia virus (CAV), designated IR CAV, was isolated in the Marek’s disease virus-transformed lymphoblastoid cell line MDCC-MSB1 (MSB1) culture for the first time. The full-length CAV DNA of this strain was cloned in the bacterial plasmid pTZ57R/T to create the molecular clone pTZ-CAV. The nucleotide and deduced amino acid sequences of viral proteins of IR CAV were compared with those of representative CAV sequences including reference and commercial vaccine strains. IR CAV was not related to vaccine strains and also found to have glutamine at positions 139 and 144 confirming previous studies in which such mutations were associated with a slow rate of virus spread in cell culture. pTZ-CAV was digested with PstI to release IR CAV DNA and then transfected into MSB1 cell by electroporation. The transfected cells showed cytopathic effect similar to virion-initiated infection. One-day old specific pathogen-free chicks were inoculated with the regenerated virus, which had been obtained from transfected MSB1 cells, and compared with the chicks inoculated with IR CAV. Gross lesions in the birds inoculated with the regenerated virus illustrated the infectious nature of the regenerated virus from the cloned IR CAV DNA.

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References

  1. M.J. Adams, E.J. Lefkowitz, A.M. King, B. Harrach, R.L. Harrison, N.J. Knowles, A.M. Kropinski, M. Krupovic, J.H. Kuhn, A.R. Mushegian, M. Nibert, S. Sabanadzovic, H. Sanfacon, S.G. Siddell, P. Simmonds, A. Varsani, F.M. Zerbini, A.E. Gorbalenya, A.J. Davison, Arch. Virol. 161, 2921–2949 (2016)

    Article  CAS  PubMed  Google Scholar 

  2. K.A. Schat, Curr. Top. Microbiol. 331, 151–183 (2009)

    CAS  Google Scholar 

  3. K.A. Schat, V.L. van Santen, in Diseases of poultry, ed. by D.E. Swayne, J.R. Glisson, L.R. McDougald, L.K. Nolan, D.L. Suarez, V.L. Nair (Wiley-Blackwell, Ames, 2013), pp. 248–264

    Google Scholar 

  4. C.R. Pope, Vet. Immunol. Immunopathol. 30, 51–65 (1991)

    Article  CAS  PubMed  Google Scholar 

  5. T. Taniguchi, N. Yuasa, M. Maeda, T. Horiuchi, Natl. Inst. Anim. Health Q (Tokyo) 23, 1–12 (1983)

    CAS  Google Scholar 

  6. N. Yuasa, T. Taniguchi, I. Yoshida, Avian Dis., 366–385 (1979)

  7. N. Yuasa, T. Taniguchi, M. Goda, M. Shibatani, T. Imada, H. Hihara, Natl. Inst. Anim. Health Q (Tokyo) 23, 75–77 (1983)

    CAS  Google Scholar 

  8. M.H. Noteborn, G. Koch, Avian Pathol. 24, 11–31 (1995)

    Article  CAS  PubMed  Google Scholar 

  9. S. Aghakhan, N. Abshar, S.R.N. Fereidouni, C. Marunesi, M. Khodashenas, Arch. Razi Inst., 1–10 (1994)

  10. A. Ezzi, A. Shoushtari, H. Marjanmehr, R. Toroghi, A. Tavasoly, M. Bahmaninejad, Arch. Razi Inst. 67, 13–19 (2012)

    Google Scholar 

  11. M. Farhoodi, R. Toroghi, M.R. Bassami, M. Kianizadeh, Arch. Razi Inst. 62, 1–6 (2007)

    CAS  Google Scholar 

  12. M. Gholami-Ahangaran, Vet. Ital. 51, 211–215 (2015)

    PubMed  Google Scholar 

  13. I. Karimi, M. Mahzounieh, S. Bahadoran, F. Azad, Comp. Clin. Path. 19, 63–67 (2010)

    Article  Google Scholar 

  14. M. Mahzounieh, I. Karimi, S. Zahraei, Int. J. Poult. Sci 4, 500–503 (2005)

    Article  Google Scholar 

  15. H. Nayabian, K. Mardani, Avian Pathol. 42, 108–113 (2013)

    Article  CAS  PubMed  Google Scholar 

  16. V.L. van Santen, H. Toro, F.J. Hoerr, Avian Dis. 51, 66–77 (2007)

    Article  PubMed  Google Scholar 

  17. M.S. McNulty, D. Todd, in A laboratory manual for the isolation, identification, and characterization of avian pathogens, ed. by L. Dufour-Zavala, D.E. Swayne, J.R. Glisson, J.E. Pearson, J.C. Reed, D.J. Jackwood, P.R. Woolcock (American Association of Avian Pathologists, Athens, GA, 2008), pp. 124–127

  18. R.W. Renshaw, C. Soine, T. Weinkle, P.H. O’Connell, K. Ohashi, S. Watson, B. Lucio, S. Harrington, K.A. Schat, J. Virol. 70, 8872–8878 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  19. M.R. Islam, R. Johne, R. Raue, D. Todd, H. Muller, J. Vet. Med. B Infect. Dis Vet Public Health 49, 332–337 (2002)

    Article  CAS  PubMed  Google Scholar 

  20. T.A. Hall, Nucl. Acid S. 41, 95–98 (1999)

    CAS  Google Scholar 

  21. K. Tamura, G. Stecher, D. Peterson, A. Filipski, S. Kumar, Mol. Biol. Evol. 30, 2725–2729 (2013)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. A. Kaffashi, C.N. Pagel, A.H. Noormohammadi, G.F. Browning, Arch. Virol. 160, 2557–2563 (2015)

    Article  CAS  PubMed  Google Scholar 

  23. S.R. Collet, in Disease of poultry, ed. by D.E. Swayne, J.R. Glisson, L.R. McDougald, L.K. Nolan, D.L. Suarez, V.L. Nair (Wiley-Blackwell, Ames, 2013), pp. 4–40

  24. M.F. Ducatez, A.A. Owoade, J.O. Abiola, C.P. Muller, Arch. Virol. 151, 97–111 (2006)

    Article  CAS  PubMed  Google Scholar 

  25. E.O. Nogueira, A.J.P. Ferreira, R. Martins Soares, E. Luiz Durigon, S. Lazzarin, L. Brentano, Comp. Immunol. Microbiol. Infect. Dis. 30, 81–96 (2007)

  26. M.F. Ducatez, H. Chen, Y. Guan, C.P. Muller, Avian Dis. 52, 68–73 (2008)

    Article  CAS  PubMed  Google Scholar 

  27. M. AboElkhair, A.G. Abd El-Razak, A.E. Metwally. Molecular characterization of chicken anemia virus circulating in chicken flocks in Egypt. Adv. Virol (2014)

  28. H.R. Kim, Y.K. Kwon, Y.C. Bae, J.K. Oem, O.S. Lee, Poult. Sci. 89, 2426–2431 (2010)

    Article  PubMed  Google Scholar 

  29. C.J. Snoeck, G.F. Komoyo, B.P. Mbee, E. Nakoune, A. Le Faou, M.P. Okwen, C.P. Muller, Virol. J. 9, 189 (2012)

    Article  PubMed  PubMed Central  Google Scholar 

  30. H.K. Brown, G.F. Browning, P.C. Scott, B.S. Crabb, Aust. Vet. J. 78, 637–640 (2000)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was funded by Research Grant No. 2-18-18-87084 from the Directory of Research, Razi Vaccine and Serum Research Institute. The authors would like to thank Michael Wilson for his careful reading of the manuscript and insightful suggestions.

Author contributions

This work was conceived, designed, performed and written by Amir Kaffashi. Fatemeh Eshratabadi helped in performing experiments. Abdelhamed Shoushtari contributed materials and reagents, supervised the development of the work and evaluated the manuscript.

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Authors and Affiliations

  1. Razi Vaccine and Serum Research Institute, Hesarak, Karaj, Iran

    Amir Kaffashi, Fatemeh Eshratabadi & Abdelhamed Shoushtari

Authors
  1. Amir Kaffashi
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  2. Fatemeh Eshratabadi
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  3. Abdelhamed Shoushtari
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Correspondence to Amir Kaffashi.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and institutional guidelines for the use and care of animals were followed.

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Edited by William Dundon.

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Kaffashi, A., Eshratabadi, F. & Shoushtari, A. Full-length infectious clone of an Iranian isolate of chicken anemia virus. Virus Genes 53, 312–316 (2017). https://doi.org/10.1007/s11262-016-1417-8

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  • DOI: https://doi.org/10.1007/s11262-016-1417-8

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