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Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China

Virus Genes volume 51, pages 298–301 (2015)Cite this article

Abstract

Canine distemper virus (CDV) is a major pathogen not only in raccoon dogs but also in a variety of carnivorous animals, including domesticated animals, particularly if they have not been vaccinated. In this study, a wild-type strain of CDV was isolated from lung tissue from a raccoon dog kept at a fur farm in Jilin Province, China. Cytopathic effects typical of CDV infection were observed after three blind passages in Vero cells, yielding a virus titer of 104.6 TCID50/mL. Virus identification was carried out by RT-PCR, immunofluorescence, electron microscopy, and genome sequencing. The results showed that the isolated virus, termed the SY strain, corresponded to the Asia-1 genotype of CDV and has a genome of 15,690 nucleotides. This represents the first complete nucleotide sequence of a CDV strain circulating in raccoon dogs in China.

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Acknowledgments

We thank Yifeng Yang and Baozeng Xu for technical assistance with confocal microscopy. The work was supported by Jilin Provincial Natural Science Foundation (No. 20140101029JC), Jilin Provincial Special Economic Animal Biological Products Technology Innovation Center, Hebei Science and Technology Support Program (No. 13826616D), and a Grant from Hebei Provincial Bureau of Animal Husbandry and Veterinary Science (No. 2011-1-19).

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Affiliations

  1. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, 130112, China

    Yuening Cheng, Jianke Wang, Miao Zhang, Jianjun Zhao, Xiqun Shao, Hang Zhao, Peng Lin & Hua Wu

  2. Hebei Normal University of Science and Technology, Changli, 066600, China

    Zengjun Ma

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  1. Yuening Cheng
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  2. Jianke Wang
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  7. Hang Zhao
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Correspondence to Jianke Wang.

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Edited by Zhen F. Fu.

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Cheng, Y., Wang, J., Zhang, M. et al. Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China. Virus Genes 51, 298–301 (2015). https://doi.org/10.1007/s11262-015-1236-3

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  • DOI: https://doi.org/10.1007/s11262-015-1236-3

Keywords

  • Canine distemper virus
  • Raccoon dog
  • Genome sequence
  • H gene