Skip to main content

Genetic diversity of fusion gene (ORF 117), an analogue of vaccinia virus A27L gene of capripox virus isolates


The fusion gene (ORF 117) sequences of twelve (n = 12) capripox virus isolates namely sheeppox (SPPV) and goatpox (GTPV) viruses from India were demonstrated for their genetic and phylogenetic relationship among them. All the isolates were confirmed for their identity by routine PCR before targeting ORF 117 gene for sequence analysis. The designed primers specifically amplified ORF 117 gene as 447 bp fragment from total genomic DNA extracted from all the isolates. Sequence analysis revealed a significant percentage of identity among GTPV, SPPV and between them at both nucleotide and amino acid levels. The topology of the phylogenetic tree revealed that three distinct clusters corresponding to SPPV, GTPV and lumpy skin disease virus was formed. However, SPPV Pune/08 and SPPV Roumanian Fanar isolates were clustered into GTPV group as these two isolates showed a 100 and 99.3 % identity with GTPV isolates of India at nt and aa levels, respectively. Protein secondary structure and 3D view was predicted and found that it has high antigenic index and surface probability with low hydrophobicity, and it can be targeted for expression and its evaluation to explore its diagnostic potential in epidemiological investigation in future.

This is a preview of subscription content, access via your institution.

Fig. 1


  1. 1.

    V. Bhanuprakash, M. Hosamani, R.K. Singh, Antivir. Res. 91, 225 (2011)

    Article  CAS  PubMed  Google Scholar 

  2. 2.

    M. Hosamani, B. Mondal, P.A. Tembhurne, S.K. Bandyopadhyay, R.K. Singh, T.J. Rasool, Virus Genes 29, 73 (2004)

    Article  CAS  PubMed  Google Scholar 

  3. 3.

    V. Bhanuprakash, B.K. Indrani, M. Hosamani, R.K. Singh, Comp. Immunol. Microbiol. Infect. Dis. 29, 27 (2006)

    Article  CAS  PubMed  Google Scholar 

  4. 4.

    S. Babiuk, T.R. Bowden, G. Parkyn, B. Dalman, D.M. Hoa, N.T. Long, P.P. Vu, D.X. Bieu, J. Copps, D.B. Boyle, J. Gen. Virol. 90, 105 (2009)

    Article  CAS  PubMed  Google Scholar 

  5. 5.

    V. Bhanuprakash, G. Venkatesan, V. Balamurugan, M. Hosamani, R. Yogisharadhya, R.S. Chauhan, A. Pande, B. Mondal, R.K. Singh, Transbound. Emerg. Dis. 57, 375 (2010)

    Article  CAS  PubMed  Google Scholar 

  6. 6.

    M.G. Garner, S.D. Sawarkar, E.K. Brett, J.R. Edwards, V.B. Kulkarni, D.B. Boyle, S.N. Singh, Trop. Anim. Health Prod. 32, 205 (2000)

    Article  CAS  PubMed  Google Scholar 

  7. 7.

    C.S. Chung, J.C. Hsaio, Y.C. Chang, W. Chang, J. Virol. 72, 1577 (1998)

    PubMed Central  CAS  PubMed  Google Scholar 

  8. 8.

    C. Lai, S. Gong, M. Esteban, J. Biol. Chem. 265, 22174 (1980)

    Google Scholar 

  9. 9.

    E.R. Tulman, C.L. Afonso, Z. Lu, L. Zsak, J.H. Sur, N.T. Sandybaev, U.Z. Kerembekova, V.L. Zaitsev, G.F. Kutish, D.L. Rock, J. Virol. 76, 6054 (2002)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. 10.

    V. Balamurugan, K.D. Jayappa, M. Hosamani, V. Bhanuprakash, G. Venkatesan, R.K. Singh, J. Vet. Diagn. Invest. 21, 225 (2009)

    Article  PubMed  Google Scholar 

  11. 11.

    G. Venkatesan, V. Balamurugan, R. Yogisharadhya, A. Kumar, V. Bhanuprakash, Virol. Sin. 27(6), 353 (2012)

    Article  PubMed  Google Scholar 

  12. 12.

    K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731 (2011)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. 13.

    L.A. Kelley, M.J.E. Sternberg, Nat. Prot. 4, 363 (2009)

    Article  CAS  Google Scholar 

  14. 14.

    M.P. Sole, B. Massimiliano, Z. Carlo, D.G.M. Carlo, I. Elena, R. Antonio, J. Transl. Med. 11, 95 (2013)

    Article  Google Scholar 

  15. 15.

    G. Venkatesan, V. Balamurugan, R.K. Singh, V. Bhanuprakash, Trop. Anim. Health Prod. 42, 1053 (2010)

    Article  CAS  PubMed  Google Scholar 

  16. 16.

    C. Le Goff, C.E. Lamien, E. Fakhfakh, A. Chadeyras, E. Aba Adulugba, G. Libeau, E. Tuppurainen, D.B. Wallace, T. Adam, R. Silber, V. Gulyaz, H. Madani, P. Caufour, S. Hammami, A. Diallo, E. Albina, J. Gen. Virol 90, 1967 (2009)

    Article  PubMed  Google Scholar 

Download references


The authors thank the Director, Indian Veterinary Research Institute (IVRI), Izatnagar for providing all the facilities to carry out this research work and to staff of pox virus laboratory, Division of Virology, IVRI, Mukteswar for their help and technical assistance. Authors also thank Joint Director, CADRAD, IVRI for regularly sending clinical samples to the Division of Virology for viral disease diagnoses.

Conflict of interest

All the authors declare that they have no conflict of interest.

Author information



Corresponding author

Correspondence to Gnanavel Venkatesan.

Additional information

M. Dashprakash and Gnanavel Venkatesan contributed equally.

Edited by Joachim Jakob Bugert.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Dashprakash, M., Venkatesan, G., Ramakrishnan, M.A. et al. Genetic diversity of fusion gene (ORF 117), an analogue of vaccinia virus A27L gene of capripox virus isolates. Virus Genes 50, 325–328 (2015).

Download citation


  • Capripox virus
  • Fusion gene
  • PCR
  • Sequence analysis
  • Phylogenetic tree