Virus Genes

, Volume 50, Issue 2, pp 253–259 | Cite as

Characterization of virus-like particles and identification of capsid proteins in Xanthophyllomyces dendrorhous

  • Oriana Flores
  • Jennifer Alcaíno
  • María Fernandez-Lobato
  • Víctor Cifuentes
  • Marcelo Baeza


Two dsRNAs of estimated lengths of 5 (L1) and 3.7 (L2) kpb are commonly found in strains of the basidiomycetous yeast Xanthophyllomyces dendrorhous, and the presence of virus-like particles (VLPs) have been described in some strains. Recently, two putative totiviruses (XdV-L1A and XdV-L1B) were identified from L1 dsRNA and one (XdV-L2) from L2 dsRNA in the strain UCD 67-385. In some strains, there are smaller dsRNAs (0.9–1.4 kb) that probable are satellite elements. In this work, the VLPs from several strains of X. dendrorhous, which differ in their dsRNAs content, were separated by sucrose gradient and characterized in relation to the dsRNAs and proteins that compose them. It was found that all types of dsRNAs were encapsidated into VLPs, supporting the hypothesis that the smaller dsRNAs are satellite molecules. A main protein of approx. 76 or 37 kDa composed the virions that only have the L1-dsRNA or L2-dsRNA, respectively. In the strain UCD 67-385, these both proteins were identified as viral capsid protein (CP), allow to confirm the gag predicted ORFs in XdV-L1A, XdV-L1B, and XdV-L2, with CPs of 76.6, 76.2, and 38.8 kDa, respectively. Analysis of predicted structures of CPs of XdV-L1A and XdV-L1B, showed high similitudes with the CPs of ScV-L-A and other totiviruses.


X. dendrorhous Mycovirus Totivirus dsRNAs encapsidation 



This work was supported by a Grant from Fondecyt (11060157), and a CONICYT Scholarships to Oriana Flores.


  1. 1.
    P. Mertens, Virus Res. 101, 3–13 (2004)CrossRefPubMedGoogle Scholar
  2. 2.
    W. Magliani, S. Conti, M. Gerloni, D. Bertolotti, L. Polonelli, Clin. Microbiol. Rev. 10, 369–400 (1997)PubMedCentralPubMedGoogle Scholar
  3. 3.
    J.D. Dinman, T. Icho, R.B. Wickner, Proc. Natl. Acad. Sci. USA. 88, 174–178 (1991)CrossRefPubMedCentralPubMedGoogle Scholar
  4. 4.
    T. Fujimura, J.C. Ribas, A.M. Makhov, R.B. Wickner, Nature 359, 746–749 (1992)CrossRefPubMedGoogle Scholar
  5. 5.
    R.B. Wickner, C.C. Wang, J.L. Patterson, in Virus Taxonomy: classification and Nomenclature of Viruses: eighth Report of the International Committee on the Taxonomy of Viruses, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Elsevier Academic Press, San Diego, 2005), pp. 571–580Google Scholar
  6. 6.
    M.J. Schmitt, F. Breinig, Nat. Rev. Microbiol. 4, 212–221 (2006)CrossRefPubMedGoogle Scholar
  7. 7.
    R. Esteban, R.B. Wickner, Mol. Cell. Biol. 6, 1552–1561 (1986)PubMedCentralPubMedGoogle Scholar
  8. 8.
    T. Fujimura, R. Esteban, Proc. Natl. Acad. Sci. USA. 108, 17667–17671 (2011)CrossRefPubMedCentralPubMedGoogle Scholar
  9. 9.
    T. Fujimura, R. Esteban, J. Biol. Chem. 287, 12797–12804 (2012)CrossRefPubMedCentralPubMedGoogle Scholar
  10. 10.
    T. Fujimura, R. Esteban, J. Biol. Chem. 288, 23716–23724 (2013)CrossRefPubMedCentralPubMedGoogle Scholar
  11. 11.
    A. Castillo, V. Cifuentes, Curr. Genet. 26, 364–368 (1994)CrossRefPubMedGoogle Scholar
  12. 12.
    I. Pfeiffer, J. Kucsera, J. Varga, A. Parducz, L. Ferenczy, Curr. Genet. 30, 294–297 (1996)CrossRefPubMedGoogle Scholar
  13. 13.
    I. Pfeiffer, J. Litter, J. Kucsera, Acta Biol. Hung. 52, 299–306 (2001)CrossRefPubMedGoogle Scholar
  14. 14.
    M. Baeza, N. Bravo, M. Sanhueza, O. Flores, P. Villarreal, V. Cifuentes, Virol J 9, 140 (2012)CrossRefPubMedCentralPubMedGoogle Scholar
  15. 15.
    M. Baeza, M. Sanhueza, O. Flores, V. Oviedo, D. Libkind, V. Cifuentes, Virol J 6, 160 (2009)CrossRefPubMedCentralPubMedGoogle Scholar
  16. 16.
    M. Baeza, M. Fernández-Lobato, V. Cifuentes, in Microbial Carotenoids from Fungi, ed. by J.L. Barredo (Humana Press, New York, 2012), pp. 195–205CrossRefGoogle Scholar
  17. 17.
    A. Gattiker, W.V. Bienvenut, A. Bairoch, E. Gasteiger, Proteomics 2, 1435–1444 (2002)CrossRefPubMedGoogle Scholar
  18. 18.
    E. Gasteiger, C. Hoogland, A. Gattiker, M.R. Wilkins, R.D. Appel, A. Bairoch, in The Proteomics Protocols Handbook, ed. by J.M. Walker (Humana Press, New York, 2005), pp. 571–607CrossRefGoogle Scholar
  19. 19.
    M.A. Kurowski, Nucleic Acids Res. 31, 3305–3307 (2003)CrossRefPubMedCentralPubMedGoogle Scholar
  20. 20.
    D.J. Taylor, J. Bruenn, BMC Biol. 7, 88 (2009)CrossRefPubMedCentralPubMedGoogle Scholar
  21. 21.
    K.A. Bostian, J.A. Sturgeon, D.J. Tipper, J. Bacteriol. 143, 463–470 (1980)PubMedCentralPubMedGoogle Scholar
  22. 22.
    D.J. Tipper, M.J. Schmitt, Mol. Microbiol. 5, 2331–2338 (1991)CrossRefPubMedGoogle Scholar
  23. 23.
    D.J. Thiele, E.M. Hannig, M.J. Leibowitz, Mol. Cell. Biol. 4, 92–100 (1984)PubMedCentralPubMedGoogle Scholar
  24. 24.
    R. Esteban, R.B. Wickner, J. Virol. 62, 1278–1285 (1988)PubMedCentralPubMedGoogle Scholar
  25. 25.
    J.R. Caston, B.L. Trus, F.P. Booy, R.B. Wickner, J.S. Wall, A.C. Steven, J. Cell Biol. 138, 975–985 (1997)CrossRefPubMedCentralPubMedGoogle Scholar
  26. 26.
    R.B. Wickner, Microbiol. Rev. 60, 250–265 (1996)PubMedCentralPubMedGoogle Scholar
  27. 27.
    D.J. Taylor, M.J. Ballinger, S.M. Bowman, J.A. Bruenn, PeerJ 1, e50 (2013)CrossRefPubMedCentralPubMedGoogle Scholar
  28. 28.
    S.E. Dunn, H. Li, G. Cardone, M.L. Nibert, S.A. Ghabrial, T.S. Baker, PLoS Pathog. 9, e1003225 (2013)CrossRefPubMedCentralPubMedGoogle Scholar
  29. 29.
    H. Naitow, J. Tang, M. Canady, R.B. Wickner, J.E. Johnson, Nat. Struct. Biol. 9, 725–728 (2002)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Oriana Flores
    • 1
  • Jennifer Alcaíno
    • 1
  • María Fernandez-Lobato
    • 2
  • Víctor Cifuentes
    • 1
  • Marcelo Baeza
    • 1
  1. 1.Laboratorio de Genética, Departamento de Ciencias Ecológicas, Facultad de CienciasUniversidad de ChileSantiagoChile
  2. 2.Centro de Biología Molecular Severo Ochoa (UAM-CSIC)Universidad Autónoma de MadridMadridSpain

Personalised recommendations