Virus Genes

, Volume 50, Issue 2, pp 316–320 | Cite as

Genetic characterization of a rare H12N3 avian influenza virus isolated from a green-winged teal in Japan

  • Vuong Nghia Bui
  • Haruko OgawaEmail author
  • Islam T. M. Hussein
  • Nichola J. Hill
  • Dai Quang Trinh
  • Mohammed AboElkhair
  • Serageldeen Sultan
  • Eric Ma
  • Keisuke Saito
  • Yukiko Watanabe
  • Jonathan A. Runstadler
  • Kunitoshi Imai


This study reports on the genetic characterization of an avian influenza virus, subtype H12N3, isolated from an Eurasian green-winged teal (Anas crecca) in Japan in 2009. The entire genome sequence of the isolate was analyzed, and phylogenetic analyses were conducted to characterize the evolutionary history of the isolate. Phylogenetic analysis of the hemagglutinin and neuraminidase genes indicated that the virus belonged to the Eurasian-like avian lineage. Molecular dating indicated that this H12 virus is likely a multiple reassortant influenza A virus. This is the first reported characterization of influenza A virus subtype H12N3 isolated in Japan and these data contribute to the accumulation of knowledge on the genetic diversity and generation of novel influenza A viruses.


Avian influenza Wild birds H12N3 Genetic Japan 



We thank Sachiko Matsuda, Obihiro University of Agriculture and Veterinary Medicine for technical support. This work was partially supported by a Grant-in- Aid for the Bilateral Joint Projects of the Japan Society for the Promotion of Science, Japan, the Heiwa Nakajima Foundation, Japan. This work was also partially supported by the US National Institute of Allergy and Infectious Diseases (NIAID contracts HHSN266200700009C and HHSN266200700007C). M. AboElkhair and S. Sultan are postdoctoral researchers from Egypt, University of Sadat City, Faculty of Veterinary Medicine and South Valley University, Faculty of Veterinary Medicine, respectively.


  1. 1.
    D.E. Swayne, D.A. Halvorson, in Influenza, ed. By Y.M. Saif, A.M. Fadly, J.M. Glisson, L.R. McDougald, L. K. Nolan, D.E. Swayne (Blackwell, Iowa, 2008), pp. 153–184Google Scholar
  2. 2.
    D.J. Alexander, Vet. Microbiol. 74, 3–13 (2000)CrossRefPubMedGoogle Scholar
  3. 3.
    R.G. Webster, R. Rott, Cell 50, 665–666 (1987)CrossRefPubMedGoogle Scholar
  4. 4.
    R.A. Fouchier, V. Munster, A. Wallensten, T.M. Bestebroer, S. Herfst, D. Smith, G.F. Rimmelzwaan, B. Olsen, A.D. Osterhaus, J. Virol. 79, 2814–2822 (2005)CrossRefPubMedCentralPubMedGoogle Scholar
  5. 5.
    R.G. Webster, W.J. Bean, O.T. Gorman, T.M. Chambers, Y. Kawaoka, Microbiol. Rev. 56, 152–179 (1992)PubMedCentralPubMedGoogle Scholar
  6. 6.
    H. Kida, R. Yanagawa, Y. Matsuoka, Infect. Immun. 30, 547–553 (1980)PubMedCentralPubMedGoogle Scholar
  7. 7.
    E. Spackman, Poult. Sci. 88, 847–850 (2009)CrossRefPubMedGoogle Scholar
  8. 8.
    S. Krauss, C.A. Obert, J. Franks, D. Walker, K. Jones, P. Seiler, L. Niles, S.P. Pryor, J.C. Obenauer, C.W. Naeve, L. Widjaja, R.J. Webby, R.G. Webster, PLoS Pathog. 3, e167 (2007)CrossRefPubMedCentralPubMedGoogle Scholar
  9. 9.
    V.J. Munster, C. Baas, P. Lexmond, J. Waldenstrom, A. Wallensten, T. Fransson, G.F. Rimmelzwaan, W.E. Beyer, M. Schutten, B. Olsen, A.D. Osterhaus, R.A. Fouchier, PLoS Pathog. 3, e61 (2007)CrossRefPubMedCentralPubMedGoogle Scholar
  10. 10.
    B. Olsen, V.J. Munster, A. Wallensten, J. Waldenstrom, A.D. Osterhaus, R.A. Fouchier, Science 312, 384–388 (2006)CrossRefPubMedGoogle Scholar
  11. 11.
    V.N. Bui, H. Ogawa, K. Karibe, K. Matsuo, T.H. Nguyen, S.S. Awad, G.L. Minoungou, Xininigen, K. Saito, Y. Watanabe, J.A. Runstadler, G.M. Happ, K. Imai, J. Vet. Med. Sci. 73, 209–215 (2011)CrossRefPubMedGoogle Scholar
  12. 12.
    V.N. Bui, H. Ogawa, L.H. Ngo, T. Baatartsogt, L.N. Abao, S. Tamaki, K. Saito, Y. Watanabe, J. Runstadler, K. Imai, Arch. Virol. 158, 451–455 (2013)CrossRefPubMedGoogle Scholar
  13. 13.
    Y. Fujimoto, H. Ito, S. Shivakoti, J. Nakamori, R. Tsunekuni, K. Otsuki, T. Ito, J. Vet. Med. Sci. 72, 963–967 (2010)CrossRefPubMedGoogle Scholar
  14. 14.
    A. Jahangir, Y. Watanabe, O. Chinen, S. Yamazaki, K. Sakai, M. Okamura, M. Nakamura, K. Takehara, Avian Dis. 52, 49–53 (2008)CrossRefPubMedGoogle Scholar
  15. 15.
    R. Manzoor, Y. Sakoda, A. Mweene, Y. Tsuda, N. Kishida, G.R. Bai, K. Kameyama, N. Isoda, K. Soda, M. Naito, H. Kida, Virus Genes 37, 144–152 (2008)CrossRefPubMedGoogle Scholar
  16. 16.
    S. Shivakoti, H. Ito, K. Otsuki, T. Ito, J. Vet. Med. Sci. 72, 459–463 (2010)CrossRefPubMedGoogle Scholar
  17. 17.
    L.N. Abao, D. Jamsransuren, V.N. Bui, L.H. Ngo, D.Q. Trinh, E. Yamaguchi, D. Vijaykrishna, J. Runstadler, H. Ogawa, K. Imai, Virus Genes 46, 323–329 (2013)CrossRefPubMedGoogle Scholar
  18. 18.
    E. Hoffmann, J. Stech, Y. Guan, R.G. Webster, D.R. Perez, Arch. Virol. 146, 2275–2289 (2001)CrossRefPubMedGoogle Scholar
  19. 19.
    O.T. Li, I. Barr, C.Y. Leung, H. Chen, Y. Guan, J.S. Peiris, L.L. Poon, J. Virol. Methods 142, 218–222 (2007)CrossRefPubMedGoogle Scholar
  20. 20.
    Y. Ha, D.J. Stevens, J.J. Skehel, D.C. Wiley, Proc. Natl. Acad. Sci. U.S.A. 98, 11181–11186 (2001)CrossRefPubMedCentralPubMedGoogle Scholar
  21. 21.
    M. Hatta, P. Gao, P. Halfmann, Y. Kawaoka, Science 293, 1840–1842 (2001)CrossRefPubMedGoogle Scholar
  22. 22.
    B. Zhou, Y. Li, R. Halpin, E. Hine, D.J. Spiro, D.E. Wentworth, J. Virol. 85, 357–365 (2011)CrossRefPubMedCentralPubMedGoogle Scholar
  23. 23.
    K. Tamura, G. Stecher, D. Peterson, A. Filipski, S. Kumar, Mol. Biol. Evol. 30, 2725–2729 (2013)CrossRefPubMedCentralPubMedGoogle Scholar
  24. 24.
    R.C. Edgar, Nucleic Acids Res. 32, 1792–1797 (2004)CrossRefPubMedCentralPubMedGoogle Scholar
  25. 25.
    A.J. Drummond, M.A. Suchard, D. Xie, A. Rambaut, Mol. Biol. Evol. 29, 1969–1973 (2012)CrossRefPubMedCentralPubMedGoogle Scholar
  26. 26.
    S.H. Olson, J. Parmley, C. Soos, M. Gilbert, N. Latorre-Margalef, J.S. Hall, P.M. Hansbro, F. Leighton, V. Munster, D. Joly, PLoS ONE 9, e90826 (2014)CrossRefPubMedCentralPubMedGoogle Scholar
  27. 27.
    E.D. Kilbourne, Emerg. Infect. Dis. 12, 9–14 (2006)CrossRefPubMedCentralPubMedGoogle Scholar
  28. 28.
    G. Neumann, T. Noda, Y. Kawaoka, Nature 459, 931–939 (2009)CrossRefPubMedCentralPubMedGoogle Scholar
  29. 29.
    E. Spackman, D.E. Swayne, M. Gilbert, D.O. Joly, W.B. Karesh, D.L. Suarez, R. Sodnomdarjaa, P. Dulam, C. Cardona, Virol. J. 6, 190 (2009)CrossRefPubMedCentralPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Vuong Nghia Bui
    • 1
  • Haruko Ogawa
    • 1
    Email author
  • Islam T. M. Hussein
    • 3
  • Nichola J. Hill
    • 3
  • Dai Quang Trinh
    • 1
  • Mohammed AboElkhair
    • 1
  • Serageldeen Sultan
    • 1
  • Eric Ma
    • 3
  • Keisuke Saito
    • 2
  • Yukiko Watanabe
    • 2
  • Jonathan A. Runstadler
    • 3
  • Kunitoshi Imai
    • 1
  1. 1.Diagnostic Center for Animal Health and Food SafetyObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  2. 2.Institute for Raptor BiomedicineKushiroJapan
  3. 3.Massachusetts Institute of TechnologyCambridgeUSA

Personalised recommendations