Genetic characterization of a rare H12N3 avian influenza virus isolated from a green-winged teal in Japan
This study reports on the genetic characterization of an avian influenza virus, subtype H12N3, isolated from an Eurasian green-winged teal (Anas crecca) in Japan in 2009. The entire genome sequence of the isolate was analyzed, and phylogenetic analyses were conducted to characterize the evolutionary history of the isolate. Phylogenetic analysis of the hemagglutinin and neuraminidase genes indicated that the virus belonged to the Eurasian-like avian lineage. Molecular dating indicated that this H12 virus is likely a multiple reassortant influenza A virus. This is the first reported characterization of influenza A virus subtype H12N3 isolated in Japan and these data contribute to the accumulation of knowledge on the genetic diversity and generation of novel influenza A viruses.
KeywordsAvian influenza Wild birds H12N3 Genetic Japan
We thank Sachiko Matsuda, Obihiro University of Agriculture and Veterinary Medicine for technical support. This work was partially supported by a Grant-in- Aid for the Bilateral Joint Projects of the Japan Society for the Promotion of Science, Japan, the Heiwa Nakajima Foundation, Japan. This work was also partially supported by the US National Institute of Allergy and Infectious Diseases (NIAID contracts HHSN266200700009C and HHSN266200700007C). M. AboElkhair and S. Sultan are postdoctoral researchers from Egypt, University of Sadat City, Faculty of Veterinary Medicine and South Valley University, Faculty of Veterinary Medicine, respectively.
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