Skip to main content

The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.)

Abstract

We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate. The complete sequence of the linear dsRNA viral genome is 13,954-nt long, contains a single 13,743 nt ORF, and presents a 149 nt 5′UTR and a 61 nt 3′UTR. The predicted ORF encodes a 4,581 aa polypeptide with a UDP-glucose glycosyl-transferase, a capsular polysaccharide synthesis protein, and a RNA-dependent RNA polymerase domain. The name yerba mate endornavirus is proposed for the identified virus. Due to the intriguing peculiarities of this virus family, and the complete lack of the yerba mate virus literature, we consider that the information reported here will be helpful in leading to a new and needed attention to this important topic and crop.

This is a preview of subscription content, access via your institution.

Fig. 1

References

  1. http://siia.gob.ar/

  2. M.J. Roossinck, S. Sabanadzovic, R. Okada, R.A. Valverde, J. Gen. Virol. 92, 2674 (2011)

    PubMed  Article  CAS  Google Scholar 

  3. Fukuhara T. and Gibbs M.J. Family Endornaviridae In “Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses”. Andrew MQ King, ed. Elsevier, pp519-521 (2012)

  4. H. Horiuchi, H. Moriyama, T. Fukuhara, Genes Genet. Syst. 78, 229 (2003)

    PubMed  Article  CAS  Google Scholar 

  5. R. Okada, E. Kiyota, S. Sabanadzovic, H. Moriyama, T. Fukuhara, P. Saha, M.J. Roossinck, A. Severin, R.A. Valverde, J. Gen. Virol. 92, 2664 (2011)

    PubMed  Article  CAS  Google Scholar 

  6. R. Okada, E. Kiyota, H. Moriyama, F. Toshiyuki, R.A. Valverde, Arch. Virol. 159, 807 (2014)

    PubMed  Article  CAS  Google Scholar 

  7. M.G. Grabherr, B.J. Haas, M. Yassour, J.Z. Levin, D.A. Thompson, I. Amit, X. Adiconis, L. Fan, R. Raychowdhury, Q. Zeng, Z. Chen, E. Mauceli, N. Hacohen, A. Gnirke, N. Rhind, F. di Palma, B.W. Birren, C. Nusbaum, K. Lindblad-Toh, N. Friedman, A. Regev, Nat. Biotechnol. 29, 644 (2011)

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  8. F. Villanueva, S. Sabanadzovic, R.A. Valverde, J. Navas-Castillo, J. Virol. 86, 1282 (2012)

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  9. D. Song, W.K. Cho, S.-H. Park, Y. Jo, K.-H. Kim, PLoS ONE 8, e64270 (2013)

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  10. Y. Espach, H.J. Maree, J.T. Burger, J. Virol. 86, 13142 (2012)

    PubMed  Article  CAS  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Humberto J. Debat.

Additional information

Nucleotide sequence accession number: The genome sequence of the proposed yerba mate endornavirus has been deposited in NCBI GenBank under accession no. KJ634409.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Debat, H.J., Grabiele, M., Aguilera, P.M. et al. The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.). Virus Genes 49, 348–350 (2014). https://doi.org/10.1007/s11262-014-1096-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-014-1096-2

Keywords

  • Yerba mate
  • Ilex paraguariensis
  • Endornavirus
  • Virus genome
  • Phylogenetic analysis