Abstract
This study reports the genetic characterization of a highly pathogenic avian influenza virus subtype H5N1 isolated from a moribund domestic duck in central Vietnam during 2012. In the moribund duck’s flock, within 6 days after vaccination with a commercial H5N1 vaccine (Re-5) to 59-day-old birds, 120 out of 2,000 ducks died. Genetic analysis revealed a substantial number of mutations in the HA gene of the isolate in comparison with the vaccine strains, Re-1 and Re-5. Similar mutations were also found in selected Vietnamese H5N1 strains isolated since 2009. Mutations in the HA gene involved positions at antigenic sites associated with antibody binding and also neutralizing epitopes, with some of the mutations resulting in the modification of N-linked glycosylation of the HA. Those mutations may be related to the escape of virus from antibody binding and the infection of poultry, interpretations which may be confirmed through a reverse genetics approach. The virus also carried an amino acid substitution in the M2, which conferred a reduced susceptibility to amantadine, but no neuraminidase inhibitor resistance markers were found in the viral NA gene. Additional information including vaccination history in the farm and the surrounding area is needed to fully understand the background of this outbreak. Such understanding and expanded monitoring of the H5N1 influenza viruses circulating in Vietnam is an urgent need to provide updated information to improve effective vaccine strain selection and vaccination protocols, aiding disease control, and biosecurity to prevent H5N1 infection in both poultry and humans.
Similar content being viewed by others
References
X. Xu, K. Subbarao, N.J. Cox, Y. Guo, Virology 261, 15–19 (1999)
I. Monne, A. Fusaro, M.H. Al-Blowi, M.M. Ismail, O.A. Khan, G. Dauphin, A. Tripodi, A. Salviato, S. Marangon, I. Capua, G. Cattoli, J. Gen. Virol. 89, 2691–2697 (2008)
S.L. Salzberg, C. Kingsford, G. Cattoli, D.J. Spiro, D.A. Janies, M.M. Aly, I.H. Brown, E. Couacy-Hymann, G.M. De Mia, H. Dung do, A. Guercio, T. Joannis, A.S. Maken Ali, A. Osmani, I. Padalino, M.D. Saad, V. Savic, N.A. Sengamalay, S. Yingst, J. Zaborsky, O. Zorman-Rojs, E. Ghedin, I. Capua, Emerg. Infect. Dis. 13, 713–718 (2007)
E. Starick, M. Beer, B. Hoffmann, C. Staubach, O. Werner, A. Globig, G. Strebelow, C. Grund, M. Durban, F.J. Conraths, T. Mettenleiter, T. Harder, Vet. Microbiol. 128, 243–252 (2008)
Y. Guan, L.L. Poon, C.Y. Cheung, T.M. Ellis, W. Lim, A.S. Lipatov, K.H. Chan, K.M. Sturm-Ramirez, C.L. Cheung, Y.H. Leung, K.Y. Yuen, R.G. Webster, J.S. Peiris, Proc. Natl. Acad. Sci. USA 101, 8156–8161 (2004)
J.S.M. Peiris, M.D. de Jong, Y. Guan, Clin. Microbiol. Rev. 20, 243–267 (2007)
H.N.E.W.G. WHO/OIE/FAO, Influenza Other Respir. Viruses 6, 1–5 (2012)
D.A. Boltz, B. Douangngeun, P. Phommachanh, S. Sinthasak, R. Mondry, C. Obert, P. Seiler, R. Keating, Y. Suzuki, H. Hiramatsu, E.A. Govorkova, R.G. Webster, J. Gen. Virol. 91, 949–959 (2010)
K. Chaichoune, W. Wiriyarat, A. Thitithanyanont, R. Phonarknguen, L. Sariya, S. Suwanpakdee, T. Noimor, S. Chatsurachai, P. Suriyaphol, K. Ungchusak, P. Ratanakorn, R.G. Webster, M. Thompson, P. Auewarakul, P. Puthavathana, J. Gen. Virol. 90, 216–222 (2009)
J. Chen, F. Fang, Z. Yang, X. Liu, H. Zhang, Z. Zhang, X. Zhang, Z. Chen, Virus Res. 146, 19–28 (2009)
L. Duan, J. Bahl, G.J. Smith, J. Wang, D. Vijaykrishna, L.J. Zhang, J.X. Zhang, K.S. Li, X.H. Fan, C.L. Cheung, K. Huang, L.L. Poon, K.F. Shortridge, R.G. Webster, J.S. Peiris, H. Chen, Y. Guan, Virology 380, 243–254 (2008)
D.C. Nguyen, T.M. Uyeki, S. Jadhao, T. Maines, M. Shaw, Y. Matsuoka, C. Smith, T. Rowe, X. Lu, H. Hall, X. Xu, A. Balish, A. Klimov, T.M. Tumpey, D.E. Swayne, L.P. Huynh, H.K. Nghiem, H.H. Nguyen, L.T. Hoang, N.J. Cox, J.M. Katz, J. Virol. 79, 4201–4212 (2005)
X.F. Wan, T. Nguyen, C.T. Davis, C.B. Smith, Z.M. Zhao, M. Carrel, K. Inui, H.T. Do, D.T. Mai, S. Jadhao, A. Balish, B. Shu, F. Luo, M. Emch, Y. Matsuoka, S.E. Lindstrom, N.J. Cox, C.V. Nguyen, A. Klimov, R.O. Donis, PLoS One 3, e3462 (2008)
T. Nguyen, P. Rivailler, C.T. Davis, T. Hoa, A. Balish, N.H. Dang, J. Jones, D.T. Vui, N. Simpson, N.T. Huong, B. Shu, R. Loughlin, K. Ferdinand, S.E. Lindstrom, I.A. York, A. Klimov, R.O. Donis, Virology 432, 405–416 (2012)
P.Q. Minh, B. Schauer, M. Stevenson, G. Jones, R.S. Morris, A. Noble, Transbound Emerg Dis. 56, 311–320 (2009)
T. Nguyen, C.T. Davis, W. Stembridge, B. Shu, A. Balish, K. Inui, H.T. Do, H.T. Ngo, X.F. Wan, M. McCarron, S.E. Lindstrom, N.J. Cox, C.V. Nguyen, A.I. Klimov, R.O. Donis, Virology 387, 250–256 (2009)
T. Dung Nguyen, T. Vinh Nguyen, D. Vijaykrishna, R.G. Webster, Y. Guan, J.S. Malik Peiris, G.J. Smith, Emerg. Infect. Dis. 14, 632–636 (2008)
World Health Organization, Cumulative number of confirmed human cases of avian influenza A(H5N1) reported to WHO. http://www.who.int/influenza/human animal interface/H5N1 cumulative_table_archives/en. Accessed 10 Dec 2013
A. Creanga, D. Thi Nguyen, N. Gerloff, H. Thi Do, A. Balish, H. Dang Nguyen, Y. Jang, V. Thi Dam, S. Thor Jones, N. Simpson, B. Shu, S. Emery, L. Berman, H.T. Nguyen, J.E. Bryant, S. Lindstrom, A. Klimov, R.O. Donis, C.T. Davis, T. Nguyen, Virology 444, 12–20 (2013)
World Health Orrganization, WHO Manual on Animal influenza diagnosis and surveillance. http://whqlibdoc.who.int/hq/2002/WHO_CDS_CSR_NCS_2002.5.pdf. Accessed 10 Jul 2012
E. Hoffmann, J. Stech, Y. Guan, R.G. Webster, D.R. Perez, Arch. Virol. 146, 2275–2289 (2001)
J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994)
K. Tamura, G. Stecher, D. Peterson, A. Filipski, S. Kumar, Mol. Biol. Evol. 30, 2725–2729 (2013)
V.N. Bui, T.D. Dao, T.T. Nguyen, L.T. Nguyen, A.N. Bui, D.Q. Trinh, N.T. Pham, K. Inui, J. Runstadler, H. Ogawa, K.V. Nguyen, K. Imai, Virus Res. 179, 125–132 (2014)
M. Hatta, P. Gao, P. Halfmann, Y. Kawaoka, Science 293, 1840–1842 (2001)
M.N. Matrosovich, T.Y. Matrosovich, T. Gray, N.A. Roberts, H.D. Klenk, Proc. Natl. Acad. Sci. USA 101, 4620–4624 (2004)
Y. Ha, D.J. Stevens, J.J. Skehel, D.C. Wiley, Proc. Natl. Acad. Sci. USA 98, 11181–11186 (2001)
M. Prabakaran, F. He, T. Meng, S. Madhan, T. Yunrui, Q. Jia, J. Kwang, J. Virol. 84, 11822–11830 (2010)
N.V. Kaverin, I.A. Rudneva, N.A. Ilyushina, N.L. Varich, A.S. Lipatov, Y.A. Smirnov, E.A. Govorkova, A.K. Gitelman, D.K. Lvov, R.G. Webster, J. Gen. Virol. 83, 2497–2505 (2002)
D.C. Wiley, I.A. Wilson, J.J. Skehel, Nature 289, 373–378 (1981)
W.L. Wu, Y. Chen, P. Wang, W. Song, S.Y. Lau, J.M. Rayner, G.J. Smith, R.G. Webster, J.S. Peiris, T. Lin, N. Xia, Y. Guan, H. Chen, J. Virol. 82, 1798–1807 (2008)
Y. Abe, E. Takashita, K. Sugawara, Y. Matsuzaki, Y. Muraki, S. Hongo, J. Virol. 78, 9605–9611 (2004)
D.J. Vigerust, V.L. Shepherd, Trends Microbiol. 15, 211–218 (2007)
W. Wang, B. Lu, H. Zhou, A.L. Suguitan Jr, X. Cheng, K. Subbarao, G. Kemble, H. Jin, J. Virol. 84, 6570–6577 (2010)
Q.M. Le, M. Kiso, K. Someya, Y.T. Sakai, T.H. Nguyen, K.H. Nguyen, N.D. Pham, H.H. Ngyen, S. Yamada, Y. Muramoto, T. Horimoto, A. Takada, H. Goto, T. Suzuki, Y. Suzuki, Y. Kawaoka, Nature 437, 1108 (2005)
E.A. Govorkova, T. Baranovich, P. Seiler, J. Armstrong, A. Burnham, Y. Guan, M. Peiris, R.J. Webby, R.G. Webster, Antiviral Res. 98, 297–304 (2013)
C.L. Cheung, J.M. Rayner, G.J. Smith, P. Wang, T.S. Naipospos, J. Zhang, K.Y. Yuen, R.G. Webster, J.S. Peiris, Y. Guan, H. Chen, J. Infect. Dis. 193, 1626–1629 (2006)
H. Suzuki, R. Saito, H. Masuda, H. Oshitani, M. Sato, I. Sato, J Infect Chemother. 9, 195–200 (2003)
S.H. Seo, E. Hoffmann, R.G. Webster, Nat. Med. 8, 950–954 (2002)
P. Jiao, G. Tian, Y. Li, G. Deng, Y. Jiang, C. Liu, W. Liu, Z. Bu, Y. Kawaoka, H. Chen, J. Virol. 82, 1146–1154 (2008)
J.C. Obenauer, J. Denson, P.K. Mehta, X. Su, S. Mukatira, D.B. Finkelstein, X. Xu, J. Wang, J. Ma, Y. Fan, K.M. Rakestraw, R.G. Webster, E. Hoffmann, S. Krauss, J. Zheng, Z. Zhang, C.W. Naeve, Science 311, 1576–1580 (2006)
Acknowledgments
We would like to thank staffs in National Institute of Veterinary Research (NIVR), Vietnam, for their technical assistance. This work was partially supported by a Grant-in-Aid for the Bilateral Joint Projects of the Japan Society for the Promotion of Science, Japan, the Heiwa Nakajima Foundation, Japan, the US National Institute of Allergy and Infectious Diseases (NIAID contracts HHSN2662007000010C), and with the contribution from the NIVR project funded by MARD of Vietnam on the Study to generate poultry influenza inactivated H5N1 vaccine derived from the local isolates of Vietnam.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bui, V.N., Ogawa, H., Trinh, D.Q. et al. Genetic characterization of an H5N1 avian influenza virus from a vaccinated duck flock in Vietnam. Virus Genes 49, 278–285 (2014). https://doi.org/10.1007/s11262-014-1089-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-014-1089-1