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Detection of enterovirus genome sequence from diarrheal feces of goat

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Abstract

Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5′-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77–87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.

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Acknowledgments

This work was supported by JSPS KAKENHI Number 23580451.

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Authors and Affiliations

  1. Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan

    Tsutomu Omatsu, Shinobu Tsuchiaka, Sachiko Okazaki, Yukie Katayama, Mami Oba, Naomi Nishiura, Yukiko Sassa, Tetsuya Furuya, Makoto Nagai & Tetsuya Mizutani

  2. The United Graduate School of Veterinary Sciences, Gifu University, Yanagito, Gifu, Gifu, 501-1193, Japan

    Tsutomu Omatsu, Shinobu Tsuchiaka, Sachiko Okazaki, Tetsuya Furuya, Makoto Nagai & Tetsuya Mizutani

  3. Department of Information Engineering, University of the Ryukyus, Nishihara, Nakagami, Okinawa, 903-0213, Japan

    Teppei Hirata & Shirou Tamaki

  4. Department of Electrical and Electronic Engineering, University of the Ryukyus, Nishihara, Nakagami, Okinawa, 903-0213, Japan

    Yasushi Shiroma

  5. Laboratory of Microbiology, Department of Veterinary Medicine Faculty and Agriculture, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan

    Tetsuya Furuya

  6. Laboratory of Epizootiology, Department of Veterinary Medicine Faculty and Agriculture, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan

    Makoto Nagai

  7. Research Institute of Biosciences, Azabu University, School of Veterinary Medicine, 1-17-71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa, 252-5201, Japan

    Hideharu Ochiai

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  1. Tsutomu Omatsu
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  2. Shinobu Tsuchiaka
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  3. Teppei Hirata
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  4. Yasushi Shiroma
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  5. Sachiko Okazaki
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  6. Yukie Katayama
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  7. Mami Oba
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  8. Naomi Nishiura
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  9. Yukiko Sassa
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  10. Tetsuya Furuya
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  11. Makoto Nagai
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  12. Hideharu Ochiai
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  13. Shirou Tamaki
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  14. Tetsuya Mizutani
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Corresponding author

Correspondence to Tetsuya Mizutani.

Additional information

Tsutomu Omatsu and Shinobu Tsuchiaka contributed equally to this work.

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Omatsu, T., Tsuchiaka, S., Hirata, T. et al. Detection of enterovirus genome sequence from diarrheal feces of goat. Virus Genes 48, 550–552 (2014). https://doi.org/10.1007/s11262-014-1057-9

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  • DOI: https://doi.org/10.1007/s11262-014-1057-9

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