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Homogenous HIV-1 subtype B quasispecies in Brazilian men and women recently infected via heterosexual transmission

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Abstract

HIV has extraordinary genetic mutability, both among individuals and at the population level. However, studies of primary HIV-1 infection and serum-converters indicate that the viral population is homogeneous at the sequence level, which suggests clonal HIV transmission. It remains unclear whether this feature applies to the female population. Ten single genome amplification sequences were generated from ten individuals (five females) with recent heterosexually acquired HIV infection as determined by the serologic testing algorithm for recent HIV seroconversion. Intra-individual genetic diversity was equally low in both genders (<2 %), with mean and median variations of 0.8 and 0 %, respectively. All of the subjects were infected with clade B. Three subjects (two females) appeared to be infected by two related viral populations, and four subjects harbored non-R5 strains. Our results support the hypothesis of clonal selection for sexual transmission of HIV-1 in both genders. Future studies that generate a larger number of clones, preferably by next generation deep sequencing, are needed to confirm these results.

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Acknowledgments

This work was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo (98/14381-4).

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Authors and Affiliations

  1. Paulista School of Medicine, Federal University of Sao Paulo, São Paulo, SP, Brazil

    Nancy Lima Gouveia, Michelle Camargo, Luiz Mario Ramos Janini, Maria Cecilia Araripe Sucupira & Ricardo Sobhie Diaz

  2. Centro Universitario Lusiada, Santos, SP, Brazil

    Marcos Montani Caseiro

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  1. Nancy Lima Gouveia
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  2. Michelle Camargo
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Correspondence to Ricardo Sobhie Diaz.

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Gouveia, N.L., Camargo, M., Caseiro, M.M. et al. Homogenous HIV-1 subtype B quasispecies in Brazilian men and women recently infected via heterosexual transmission. Virus Genes 48, 421–428 (2014). https://doi.org/10.1007/s11262-014-1048-x

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