Skip to main content
SpringerLink
Log in

Phylogenetic analysis of the VP1 gene of Enterovirus 71 in Guangzhou during the high occurrence period of 2008

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

An outbreak of hand, foot, and mouth disease (HFMD) in Guangzhou in 2008 affected over 10,000 children and resulted in high hospital admission rates. To investigate the molecular epidemiological pattern of EV71 infections in Guangzhou, throat swab samples were collected from 102 children clinically diagnosed with HFMD from May to July of 2008 in Guangzhou. Partial VP1 (virus protein 1) fragments of Enterovirus 71 (EV71) isolates were sequenced, and used alongside EV71 sequences entered in GenBank to construct a phylogenetic tree using MEGA5.0. Blast and phylogenetic analyses showed that all 21 sequences belonged to subgenogroup C4 of EV71. In early May, diverse strains were circulating in Guangzhou, but by July, only a small number of these strains could be detected. These results could indicate that geographic and climatic features may affect the epidemic characteristics of EV71, and that some C4 strains might retain their infectivity at higher temperatures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. S.C. Huang, Y.W. Hsu, H.C. Wang, S.W. Huang, D. Kiang, H.P. Tsai, S.M. Wang, C.C. Liu, K.H. Lin, I.J. Su, J.R. Wang, Virus Res. 131, 250–259 (2008)

    Article  CAS  PubMed  Google Scholar 

  2. S. Singh, V.T. Chow, K.P. Chan, A.E. Ling, C.L. Poh, J. Virol. Methods 88, 193–204 (2000)

    Article  CAS  PubMed  Google Scholar 

  3. P. McMinn, K. Lindsay, D. Perera, H.M. Chan, K.P. Chan, M.J. Cardosa, J. Virol. 75, 7732–7738 (2001)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. T. Vuorinen, R. Vainionpaa, T. Hyypia, Clinical infectious diseases 37, 452–455 (2003)

    Article  PubMed  Google Scholar 

  5. G. Lee, C. Lee, J microbiol. 46, 319–324 (2008)

    Article  CAS  PubMed  Google Scholar 

  6. M.S. Oberste, K. Maher, D.R. Kilpatrick, M.R. Flemister, B.A. Brown, M.A. Pallansch, J. Clin. Microbiol. 37, 1288–1293 (1999)

    CAS  PubMed Central  PubMed  Google Scholar 

  7. M.S. Oberste, W.A. Nix, K. Maher, M.A. Pallansch, J clin virol. 26, 375–377 (2003)

    Article  CAS  PubMed  Google Scholar 

  8. K.H. Lin, K.P. Hwang, G.M. Ke, C.F. Wang, L.Y. Ke, Y.T. Hsu, Y.C. Tung, P.Y. Chu, B.H. Chen, H.L. Chen, C.L. Kao, J.R. Wang, H.L. Eng, S.Y. Wang, L.C. Hsu, H.Y. Chen, J. Med. Virol. 78, 254–262 (2006)

    Article  CAS  PubMed  Google Scholar 

  9. K. Mizuta, C. Abiko, T. Murata, Y. Matsuzaki, T. Itagaki, K. Sanjoh, M. Sakamoto, S. Hongo, S. Murayama, K. Hayasaka, J. Clin. Microbiol. 43, 6171–6175 (2005)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. H. Shimizu, A. Utama, N. Onnimala, C. Li, Z. Li-Bi, M. Yu-Jie, Y. Pongsuwanna, T. Miyamura, Pediatrics international 46, 231–235 (2004)

    Article  PubMed  Google Scholar 

  11. P.V. Tu, N.T. Thao, D. Perera, T.K. Huu, N.T. Tien, T.C. Thuong, O.M. How, M.J. Cardosa, P.C. McMinn, Emerg. Infect. Dis. 13, 1733–1741 (2007)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731–2739 (2011)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. M.J. Cardosa, D. Perera, B.A. Brown, D. Cheon, H.M. Chan, K.P. Chan, H. Cho, P. McMinn, Emerg. Infect. Dis. 9, 461–468 (2003)

    Article  PubMed  Google Scholar 

  15. L. Li, Y. He, H. Yang, J. Zhu, X. Xu, J. Dong, Y. Zhu, Q. Jin, J. Clin. Microbiol. 43, 3835–3839 (2005)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. B.A. Brown, M.S. Oberste, J.P. Alexander Jr, M.L. Kennett, M.A. Pallansch, J. Virol. 73, 9969–9975 (1999)

    CAS  PubMed Central  PubMed  Google Scholar 

  17. Y.P. Huang, T.L. Lin, C.Y. Kuo, M.W. Lin, C.Y. Yao, H.W. Liao, L.C. Hsu, C.F. Yang, J.Y. Yang, P.J. Chen, H.S. Wu, Virus Res. 137, 206–212 (2008)

    Article  CAS  PubMed  Google Scholar 

  18. T. Munemura, M. Saikusa, C. Kawakami, H. Shimizu, M. Oseto, A. Hagiwara, H. Kimura, T. Miyamura, Arch. Virol. 148, 253–263 (2003)

    Article  CAS  PubMed  Google Scholar 

  19. M.Y. Liu, W. Liu, J. Luo, Y. Liu, Y. Zhu, H. Berman, J. Wu, PLoS ONE 6, e25287 (2011)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  20. X. Tan, X. Huang, S. Zhu, H. Chen, Q. Yu, H. Wang, X. Huo, J. Zhou, Y. Wu, D. Yan, Y. Zhang, D. Wang, A. Cui, H. An, W. Xu, PLoS ONE 6, e25662 (2011)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. K.T. Chen, H.L. Chang, S.T. Wang, Y.T. Cheng, J.Y. Yang, Pediatrics 120, e244–e252 (2007)

    Article  PubMed  Google Scholar 

  22. S.H. Kung, S.F. Wang, C.W. Huang, C.C. Hsu, H.F. Liu, J.Y. Yang, Clin microbiol infect. 13, 782–787 (2007)

    Article  CAS  PubMed  Google Scholar 

  23. T.N. Wu, S.F. Tsai, S.F. Li, T.F. Lee, T.M. Huang, M.L. Wang, K.H. Hsu, C.Y. Shen, Emerg. Infect. Dis. 5, 458–460 (1999)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. C. Yoke-Fun, S. AbuBakar, BMC Microbiol. 6, 74 (2006)

    Article  PubMed Central  PubMed  Google Scholar 

  25. Y.H. Kung, S.W. Huang, P.H. Kuo, D. Kiang, M.S. Ho, C.C. Liu, C.K. Yu, I.J. Su, J.R. Wang, Virology 396, 1–9 (2010)

    Article  CAS  PubMed  Google Scholar 

  26. M. Urashima, N. Shindo, N. Okabe, Jpn J infect dis. 56, 48–53 (2003)

    PubMed  Google Scholar 

  27. Li T., Yang Z., Di B., and Wang M., Epidemiology and infection, 1-10, 2013

Download references

Acknowledgments

Financial support was provided by grants from the Guangzhou Municipal Health Bureau (30307-2080048) and the Guangdong Natural Science Foundation (s2012010008319).

Author information

Authors and Affiliations

  1. Institute of Pediatrics, Guangzhou Women & Children’s Medical Center, Guangzhou, 510623, China

    Ying-xian Yin, Zhi-ying Ou, Yi Xu & Hui-min Xia

  2. Guangzhou Institute of Respiratory Diseases, Guangzhou, 510230, China

    Rong Zhou

Authors
  1. Ying-xian Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhi-ying Ou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rong Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hui-min Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ying-xian Yin.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yin, Yx., Ou, Zy., Xu, Y. et al. Phylogenetic analysis of the VP1 gene of Enterovirus 71 in Guangzhou during the high occurrence period of 2008. Virus Genes 48, 538–542 (2014). https://doi.org/10.1007/s11262-014-1046-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-014-1046-z

Keywords

Search

Navigation