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Studies on genetic diversity of bovine viral diarrhea viruses in Danish cattle herds

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Abstract

Scandinavian countries have successfully pursued bovine viral diarrhea virus (BVDV) eradication without the use of vaccines. In Denmark, control and eradication of BVDV were achieved during the last two decades, but occasionally new BVDV infections are detected in some Danish cattle herds. The aim of this study was to determine recent BVDV subtypes isolated from 4 Danish herds (A, B, C, and D) isolated in 2009–2012 and to analyze the genetic variation of these isolates within the same herd and its relation with those of other herds. The results showed that three herds (B, C, D) were BVDV 1-b and only one herd (herd A) was BVDV 1-d, no other subtypes were detected. The deduced E2 amino acids result showed a high identity percent (99–100 %) between isolates originating from the same herd, but with higher variation compared to isolates of the other herds. Some of these new Danish strains have closer relationship to BVDVs from outside Denmark than to older Danish strains indicating that these are new introductions to Denmark. In conclusion, BVDV-1 subtypes recently detected in Denmark were only subtypes 1b and 1d, and BVDV infections established in a herd is genetically stable over a long time period.

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References

  1. C.M. Fauquet, D. Fargette, Virology 2, 2–64 (2005)

    Article  Google Scholar 

  2. M.S. Collect, R. Larson, C. Gold, D. Strinck, D.K. Anderson, A.F. Purchio, Virology 165, 191–199 (1988)

    Article  Google Scholar 

  3. G. Meyers, T. Rümenapf, H.-J. Thiel, Virology 171, 555–567 (1989)

    Article  CAS  PubMed  Google Scholar 

  4. R.O. Donis, Vet Clin North Am Food Anim Pract 11, 393–423 (1995)

    CAS  PubMed  Google Scholar 

  5. P. Becher, M. Orlich, A.D. Shannon, G. Horner, M. Konig, H.J. Thiel, J Gen Virol 78, 1357–1366 (1997)

    CAS  PubMed  Google Scholar 

  6. P. Becher, M. Orlich, A. Kosmidou, M. Konig, M. Baroth, H.J. Thiel, Virology 262, 64–71 (1999)

    Article  CAS  PubMed  Google Scholar 

  7. P.A. van Rijn, H.G. van Gennip, C.H. Leendertse, C.J. Bruschke, D.J. Paton, R.J. Moormann, J.T. van Oirschot, Virology 237, 337–348 (1997)

    Article  PubMed  Google Scholar 

  8. V. Bitsch, K.E. Hansen, L. Ronsholt, Vet Microbiol 77, 137–143 (2000)

    Article  CAS  PubMed  Google Scholar 

  9. A. Uttenthal, T. Stadejek, B. Nylin, APMIS 113, 536–541 (2005)

    Article  CAS  PubMed  Google Scholar 

  10. T.B. Rasmussen, A. Uttenthal, I. Reimann, J. Nielsen, K. Depner, M. Beer, J Gen Virol 88, 481–486 (2007)

    Article  CAS  PubMed  Google Scholar 

  11. M. Tajima, H.R. Frey, O. Yamato, Y. Maede, V. Moennig, H. Scholz, I. Greiser-Wilke, Virus Res 76, 31–42 (2001)

    Article  CAS  PubMed  Google Scholar 

  12. M. Kearse, R. Moir, A. Wilson, S. Stones-Havas, M. Cheung, S. Sturrock, S. Buxton, A. Cooper, S. Markowitz, C. Duran, T. Thierer, B. Ashton, P. Meintjes, A. Drummond, Bioinformatics 28, 1647–1649 (2012)

    Article  PubMed Central  PubMed  Google Scholar 

  13. D.J. Paton, A. McGoldrick, I. Greiser-Wilke, S. Parchariyanon, J.Y. Song, P.P. Liou, T. Stadejek, J.P. Lowings, H. Bjorklund, S. Belak, Vet Microbiol 73, 137–157 (2000)

    Article  CAS  PubMed  Google Scholar 

  14. S. Vilcek, D.J. Paton, B. Durkovic, L. Strojny, G. Ibata, A. Moussa, A. Loitsch, W. Rossmanith, S. Vega, M.T. Scicluna, V. Paifi, Arch Virol 146, 99–115 (2001)

    Article  CAS  PubMed  Google Scholar 

  15. P. Lowings, G. Bata, J. Needham, D. Paton, J Gen Virol 77, 1311–1321 (1996)

    Article  CAS  PubMed  Google Scholar 

  16. P. Tijssen, C. Pellerin, J. Lecomte, H.J. van den, Virology 217, 356–361 (1996)

    Article  CAS  PubMed  Google Scholar 

  17. A. Postel, S. Schmeiser, J. Bernau, A. Meindl-Boehmer, G. Pridotkas, Z. Dirbakova, M. Mojzis, P. Becher, Vet Res 43, 50 (2012)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. M.E. Collins, M. Desport, J. Brownlie, Virology 259, 85–98 (1999)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

AN was a visiting scientist in AU’s laboratory, funded by The Partnership and Ownership initiative (ParOwn) grant, launched by the Egyptian Ministry of Higher Education and State for Scientific research.

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Authors and Affiliations

  1. National Veterinary Institute, Technical University of Denmark, Lindholm, 4771, Kalvehave, Denmark

    Abdou Nagy, Ulrik Fahnøe, Thomas Bruun Rasmussen & Åse Uttenthal

  2. Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt

    Abdou Nagy

Authors
  1. Abdou Nagy
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  2. Ulrik Fahnøe
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  3. Thomas Bruun Rasmussen
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  4. Åse Uttenthal
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Corresponding author

Correspondence to Abdou Nagy.

Additional information

Abdou Nagy (AN) performed the lab work on PCR analysis and sequencing on all samples. Åse Uttenthal (AU) hosted AN during his stay, provided the information and the sera for the study, and produced the table. Ulrik Fahnøe did the computer work on the sequences and made the figures for the paper. Thomas B. Rasmussen supervised the sequencing studies. The manuscript was drafted by AN and Co-drafted by all authors.

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Nagy, A., Fahnøe, U., Rasmussen, T.B. et al. Studies on genetic diversity of bovine viral diarrhea viruses in Danish cattle herds. Virus Genes 48, 376–380 (2014). https://doi.org/10.1007/s11262-013-1020-1

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  • DOI: https://doi.org/10.1007/s11262-013-1020-1

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