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Complete genome sequence of Courdo11 virus, a member of the family Mimiviridae

Virus Genes volume 48, pages 218–223 (2014)Cite this article

Abstract

Giant viruses of amoebae were discovered 10 years ago and led to the description of two new viral families: Mimiviridae and Marseilleviridae. These viruses exhibit remarkable features, including large capsids and genomes that are similar in size to those of small bacteria and their large genetic repertoires include genes that are unique among viruses. The family Mimiviridae has grown during the past decade since the discovery of its initial member, Mimivirus, and continues to expand. Here, we describe the genome of a new giant virus that infects Acanthamoeba spp., Courdo11 virus, isolated in 2010 by inoculating Acanthamoeba spp. with freshwater collected from a river in southeastern France. The Courdo11 virus genome is a double stranded DNA molecule composed of 1,245,674 nucleotides. The comparative analyses of Courdo11 virus with the genomes of other giant viruses showed that it belongs to lineage C of mimiviruses of amoebae, being most closely related to Megavirus chilensis and LBA 111, the first mimivirus isolated from a human. Major characteristics of the M. chilensis genome were identified in the Courdo11 virus genome, found to encode three more tRNAs. Genomic architecture comparisons mirrored previous findings that showed conservation of collinear regions in the middle part of the genome and diversity towards the extremities. Finally, fourteen ORFans were identified in the Courdo11 virus genome, suggesting that the pan-genome of mimiviruses of amoeba might reach a plateau.

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Author information

Affiliations

  1. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198, INSERM U1095, Facultés de Médecine et de Pharmacie, Aix-Marseille Univ., 27 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France

    Niyaz Yoosuf, Isabelle Pagnier, Ghislain Fournous, Catherine Robert, Bernard La Scola, Didier Raoult & Philippe Colson

  2. Fondation Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Assistance Publique – Hôpitaux de Marseille, 264 rue Saint-Pierre, 13385, Marseille Cedex 05, France

    Isabelle Pagnier, Bernard La Scola, Didier Raoult & Philippe Colson

Authors
  1. Niyaz Yoosuf
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  2. Isabelle Pagnier
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  3. Ghislain Fournous
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  4. Catherine Robert
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  5. Bernard La Scola
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  6. Didier Raoult
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  7. Philippe Colson
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Correspondence to Philippe Colson.

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Yoosuf, N., Pagnier, I., Fournous, G. et al. Complete genome sequence of Courdo11 virus, a member of the family Mimiviridae . Virus Genes 48, 218–223 (2014). https://doi.org/10.1007/s11262-013-1016-x

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  • DOI: https://doi.org/10.1007/s11262-013-1016-x

Keywords

  • Courdo11 virus
  • Mimivirus
  • Mimiviridae
  • Giant virus
  • Megavirales
  • Nucleocytoplasmic large DNA viruses
  • Amoeba