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Construction and characterization of an infectious cDNA clone of enterovirus type 71 subgenotype C4

Virus Genes volume 47, pages 235–243 (2013)Cite this article

Abstract

Enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease and induces fatal neurological complications. In recent years, this virus has become a major threat to public health in the Asia–Pacific region, while no effective antiviral therapies and vaccines are currently available. In this study, we constructed and characterized for the first time an infectious full-length EV71 cDNA clone derived from the SHZH98 strain, which was the first subgenotype C4 strain isolated in China. Our data demonstrate that the rescued EV71 viruses exhibited growth kinetics in vitro and morphologies similar to those of the BrCr-TR strain and reached a maximum titer of 107.5 TCID50/ml. Although the rescued viruses were able to infect suckling mice, no typical symptoms of EV71 infection were observed for up to 18 days post-inoculation. Taken together our research provides an important tool to study the epidemic strains of EV71 in the Asia–Pacific region and promote the development of vaccines.

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Acknowledgments

The authors thank Dr. Lawrence Kleiman at the Lady Davis Institute for proofreading. This study was supported in part by the National 973 Grant—2012CB911100 (to C.S.), the Nature Science Foundation of China—30973569 (to S.C.), the National S&T Major Special Project on Major New Drug Innovation—2012ZX09301-002-004 (to C.S.), and the Xiehe Scholar fund (to S.C.).

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Affiliations

  1. Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical School, No. 1 Tian Tan Xi Li, Beijing, 100050, People’s Republic of China

    Yong-Xin Zhang, Xiao-Yu Li, Xiao Yin, Yu-Huan Li, Ji-Wei Ding, Jin-Ming Zhou & Shan Cen

  2. Beijing Union University, Beijing, 100101, People’s Republic of China

    Tao Wei

  3. Laboratory Animal Center of the Academy of Military Medical Sciences, Beijing, 100071, People’s Republic of China

    Guang-Zhou Zhang

  4. Institute of Pathogen Biology, Chinese Academy of Medical Science and Peking Union Medical School, No. 9 Dong Dan San Tiao, Beijing, 100730, People’s Republic of China

    Qi Jin

Authors
  1. Yong-Xin Zhang
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  2. Tao Wei
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  3. Xiao-Yu Li
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  4. Xiao Yin
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  5. Yu-Huan Li
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  6. Ji-Wei Ding
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  7. Jin-Ming Zhou
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  8. Guang-Zhou Zhang
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  9. Qi Jin
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  10. Shan Cen
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Corresponding authors

Correspondence to Qi Jin or Shan Cen.

Additional information

Yong-Xin Zhang and Tao Wei have contributed equally to this study.

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Zhang, YX., Wei, T., Li, XY. et al. Construction and characterization of an infectious cDNA clone of enterovirus type 71 subgenotype C4. Virus Genes 47, 235–243 (2013). https://doi.org/10.1007/s11262-013-0955-6

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  • DOI: https://doi.org/10.1007/s11262-013-0955-6

Keywords

  • Enterovirus 71
  • Infectious cDNA clone
  • Subgenotype C4
  • SHZH98 strain