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Characterization and complete genome sequence analysis of Staphylococcus aureus bacteriophage SA12

Virus Genes volume 47, pages 389–393 (2013)Cite this article

Abstract

Staphylococcus aureus is a well-known pathogen that causes several serious diseases in humans and animals. As part of the efforts to control this pathogen, a newly isolated bacteriophage, SA12, which specifically targets S. aureus, was characterized, and its genome was completely sequenced. Host range and bacteriophage challenge tests demonstrated its specific and efficient host lysis of S. aureus. The genome of phage SA12 consists of 42,902 bp length double-stranded DNA with 58 predicted ORFs-encoding phage structure, DNA manipulation, packaging, host lysis, and regulation proteins. The characterization and genome study of phage SA12 in this report is useful for understanding S. aureus-targeting bacteriophages and provides basic information for the further development of phage-based biocontrol agents against S. aureus.

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Acknowledgements.

This research was supported by the R&D Convergence Center Support Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. Y. Chang and H. Shin were the recipients of a graduate fellowship provided by the MEST through the Brain Korea 21 Project. Nucleotide sequence accession number. The nucleotide sequence has been deposited in GenBank under accession number KC677663.

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Affiliations

  1. Department of Food and Animal Biotechnology, Seoul National University, Seoul, 151-921, Korea

    Yoonjee Chang, Hakdong Shin & Sangryeol Ryu

  2. Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Korea

    Yoonjee Chang, Hakdong Shin & Sangryeol Ryu

  3. Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Yoonjee Chang, Hakdong Shin & Sangryeol Ryu

  4. Center for Food and Bioconvergence, Seoul National University, Seoul, 151-921, Korea

    Yoonjee Chang, Hakdong Shin & Sangryeol Ryu

  5. Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 446-701, Korea

    Ju-Hoon Lee

  6. Microbial Safety Division, Rural Development Administration, National Academy of Agricultural Science, Suwon, 441-707, Korea

    Sunggi Heu

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  1. Yoonjee Chang
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  2. Ju-Hoon Lee
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  3. Hakdong Shin
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  4. Sunggi Heu
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  5. Sangryeol Ryu
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Corresponding author

Correspondence to Sangryeol Ryu.

Additional information

Y.C and J-H.L. contributed equally to this study.

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Chang, Y., Lee, JH., Shin, H. et al. Characterization and complete genome sequence analysis of Staphylococcus aureus bacteriophage SA12. Virus Genes 47, 389–393 (2013). https://doi.org/10.1007/s11262-013-0938-7

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  • DOI: https://doi.org/10.1007/s11262-013-0938-7

Keywords

  • Staphylococcus aureus
  • Bacteriophage
  • Siphoviridae
  • Host lysis
  • Genome