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Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells

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Abstract

We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.

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References

  1. E.B. Carstens, L.A. Ball, Arch. Virol. 154, 1181–1188 (2009)

    Article  PubMed  CAS  Google Scholar 

  2. S.A.B. Miele, M.J. Garavaglia, M.N. Belaich, P.D. Ghiringhelli, Int. J. Evol. Biol. (2011). doi:10.4061/2011/379424

    PubMed  Google Scholar 

  3. P.M. Zanotto, B.D. Kessing, J.E. Maruniak, J. Invertebr. Pathol. 62, 147–164 (1993)

    Article  PubMed  CAS  Google Scholar 

  4. P.D. Friesen, in The Baculoviruses, ed. by L.K. Miller (Plenum Press, New York, 1997), p. 141

  5. S.M. Rodems, P.D. Friesen, J. Virol. 69, 5368–5375 (1995)

    PubMed  CAS  Google Scholar 

  6. M.N. Pearson, R.M. Bjornson, G.D. Pearson, G.F. Rohrmann, Science 257, 1382–1384 (1992)

    Article  PubMed  CAS  Google Scholar 

  7. M. Kool, J.T.M. Voeten, R.W. Goldbach, J. Tramper, J.M. Vlak, J. Gen. Virol. 74, 2661–2668 (1993)

    Article  PubMed  CAS  Google Scholar 

  8. C. Rankin, B.G. Ooi, L.K. Miller, Gene 70, 39–49 (1988)

    Article  PubMed  CAS  Google Scholar 

  9. N.E. Huh, R.F. Weaver, J. Gen. Virol. 71, 195–202 (1990)

    Article  PubMed  CAS  Google Scholar 

  10. L.A. Guarino, B. Xu, J. Jin, W. Dong, J. Virol. 72, 7985–7991 (1998)

    PubMed  CAS  Google Scholar 

  11. A.L. Passarelli, L.A. Guarino, Curr. Drug Targets 8, 1103–1115 (2007)

    Article  PubMed  CAS  Google Scholar 

  12. A.L. Passarelli, L.K. Miller, J. Virol. 67, 2149–2158 (1993)

    PubMed  CAS  Google Scholar 

  13. J.C. Rapp, J.A. Wilson, L.K. Miller, J. Virol. 72, 10197–10206 (1998)

    PubMed  CAS  Google Scholar 

  14. M. Kool, C. Ahrens, R.W. Goldbach, G.F. Rohrmann, J.M. Vlak, Proc. Natl Acad. Sci. U.S.A. 91, 11212–11216 (1994)

    Article  PubMed  CAS  Google Scholar 

  15. A. Lu, L.K. Miller, J. Virol. 69, 975–982 (1995)

    PubMed  CAS  Google Scholar 

  16. S. Maeda, S.G. Kamita, A. Kondo, J. Virol. 67, 6234–6238 (1993)

    PubMed  CAS  Google Scholar 

  17. A. Lu, L.K. Miller, J. Virol. 69, 6265–6272 (1995)

    PubMed  CAS  Google Scholar 

  18. G.F. Rohrmann, Baculovirus Molecular Biology, 2nd edn. (Bethesda: National Center for Biotechnology Information, 2011), http://www.ncbi.nlm.nih.gov/books/NBK49500/

  19. T. Williams, D. Goulson, P. Caballero, J. Cisneros, A.M. Martínez, J.W. Chapman, D.X. Roman, R.D. Cave, Biol. Control 14, 67–75 (1999)

    Article  Google Scholar 

  20. R.W. Behle, H.J.R. Popham, J. Invertebr. Pathol. 109, 194–200 (2012)

    Article  PubMed  Google Scholar 

  21. A.H. McIntosh, C.M. Ignoffo, P.L. Andrews, Intervirology 23, 150–156 (1985)

    Article  PubMed  CAS  Google Scholar 

  22. G.M. Danyluk, J.E. Maruniak, J. Invertebr. Pathol. 50, 207–212 (1987)

    Article  PubMed  CAS  Google Scholar 

  23. H.-S. Liu, S.L. Bilimoria, Arch. Virol. 115, 101–113 (1990)

    Article  PubMed  CAS  Google Scholar 

  24. M.R. Pedrini, J.L. Caldas Wolff, S. Reid, Ann. Appl. Biol. 145, 107–112 (2004)

    Article  Google Scholar 

  25. H.-S. Liu, S.L. Bilimoria, Intervirology 40, 50–54 (1997)

    Article  PubMed  CAS  Google Scholar 

  26. J.L. Caldas Wolff, F.H. Valicente, R. Martins, J.V. Oliveira, P.M. Zanotto, J. Gen. Virol. 89, 1202–1211 (2008)

    Article  Google Scholar 

  27. R.L. Harrison, B. Puttler, H.J.R. Popham, J. Gen. Virol. 89, 775–790 (2008)

    Article  PubMed  CAS  Google Scholar 

  28. O. Simón, L. Palma, I. Beperet, D. Muñoz, M. López-Ferber, P. Caballero, T. Williams, J. Invertebr. Pathol. 107, 33–42 (2011)

    Article  PubMed  Google Scholar 

  29. J.G.M. Heldens, Y. Liu, D. Zuidema, R.W. Goldbach, J.M. Vlak, J. Gen. Virol. 78, 3101–3114 (1997)

    PubMed  CAS  Google Scholar 

  30. M.N. Pearson, G.F. Rohrmann, J. Virol. 72, 9142–9149 (1998)

    PubMed  CAS  Google Scholar 

  31. M.F. Berretta, M.L. Ríos, A. Sciocco de Cap, J. Invertebr. Pathol. 71, 280–282 (1998)

    Article  PubMed  CAS  Google Scholar 

  32. M.F. Berretta, M. Deshpande, E.A. Crouch, A.L. Passarelli, Virology 348, 175–189 (2006)

    Article  PubMed  CAS  Google Scholar 

  33. V.A. Olson, J.A. Wetter, P.D. Friesen, J. Virol. 75, 6042–6051 (2001)

    Article  PubMed  CAS  Google Scholar 

  34. R. Broer, J.G.M. Heldens, E.A. van Strien, D. Zuidema, J.M. Vlak, J. Gen. Virol. 79, 1563–1572 (1998)

    PubMed  CAS  Google Scholar 

  35. M.F. Berretta, A.L. Passarelli, Virology 355, 82–93 (2006)

    Article  PubMed  CAS  Google Scholar 

  36. S.M. Rodems, P.D. Friesen, J. Virol. 67, 5776–5785 (1993)

    PubMed  CAS  Google Scholar 

  37. J. Todd, A.L. Passarelli, L.K. Miller, J. Virol. 69, 968–974 (1995)

    PubMed  CAS  Google Scholar 

  38. E.A. Prikhod’ko, L.K. Miller, J. Virol. 70, 7116–7124 (1996)

    PubMed  Google Scholar 

  39. M.N. Pearson, G.F. Rohrmann, Virology 235, 153–165 (1997)

    Article  PubMed  CAS  Google Scholar 

  40. G.E. Chisholm, D.J. Henner, J. Virol. 62, 3193–3200 (1988)

    PubMed  CAS  Google Scholar 

  41. D.A. Theilmann, L.G. Willis, B.J. Bosch, I.J. Forsythe, Q. Li, Virology 290, 211–223 (2001)

    Article  PubMed  CAS  Google Scholar 

  42. I. Huijskens, L. Li, L.G. Willis, D.A. Theilmann, Virology 323, 120–130 (2004)

    Article  PubMed  CAS  Google Scholar 

  43. T.M. Stewart, I. Huijskens, L.G. Willis, D.A. Theilmann, J. Virol. 79, 4619–4629 (2005)

    Article  PubMed  CAS  Google Scholar 

  44. L.A. Guarino, T.A. Mistretta, W. Dong, J. Virol. 76, 12032–12043 (2002)

    Article  PubMed  CAS  Google Scholar 

  45. I. Landais, R. Vincent, M. Bouton, G. Devauchelle, M. Duonor-Cerutti, M. Ogliastro, Virology 344, 421–431 (2006)

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors thank Dr. A. Lorena Passarelli for providing the AcMNPV lef library, plasmids pBAS35K-Luc/28mer-up+/PA and pAchr5CAPluc, and for critically reading the manuscript. We are also grateful to O. Marcelo Farinón for excellent technical assistance. This study was supported by grants from the National Institute for Agricultural Technology (INTA) and the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT). MFB, OT, and VR hold research career awards from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).

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Authors and Affiliations

  1. Instituto de Microbiología y Zoología Agrícola (IMYZA), INTA Castelar, Castelar, Argentina

    Marcelo F. Berretta & Alicia Sciocco-Cap

  2. Instituto de Biotecnología (IB), INTA Castelar, Castelar, Argentina

    M. Gabriela López & Oscar Taboga

  3. Instituto de Biotecnología y Biología Molecular (IBBM, CONICET-UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina

    Víctor Romanowski

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  1. Marcelo F. Berretta
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  2. M. Gabriela López
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  3. Oscar Taboga
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  4. Alicia Sciocco-Cap
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  5. Víctor Romanowski
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Correspondence to Marcelo F. Berretta.

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Berretta, M.F., López, M.G., Taboga, O. et al. Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells. Virus Genes 46, 152–161 (2013). https://doi.org/10.1007/s11262-012-0843-5

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  • DOI: https://doi.org/10.1007/s11262-012-0843-5

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