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Detection and molecular characterization of avipoxviruses isolated from different avian species in Egypt

Abstract

Six clinical cases of avipoxvirus (APV) infection were investigated and molecular biologically studied. The samples were collected from different domesticated birds reared in the Egyptian backyard management system and were propagated on the chorioallantoic membrane of embryonated chicken eggs. The virus isolation was confirmed via PCR amplification of fpv167 (P4b) gene locus. All the studied isolates were characterized as Fowlpox-like viruses based on the amplicon length of fpv140 gene locus. The phylogenetic analysis of fpv167 (P4b) gene clustered Elsharqyia_FWPV1, Elsharqyia_FWPV2, Elsharqyia_FWPV3, Elsharqyia_FWPV4, and Elsharqyia_TKPV strains within subclade A1. Furthermore, Elsharqyia_PGPV strain was clustered within subclade A2 (Turkeypox virus) and showed 100 % nucleic acid identity with the wood pigeon Indian which was isolated in 2009. On the other hand, when the fpv140 gene was used for the phylogenetic analysis, Elsharqyia_PGPV was clustered within subclade A4 (Pigeonpox virus) with the other PGPVs. This study is considered the first molecular record for APVs circulating in the Egyptian birds. Further studies in a larger scale need to be developed to have a better understanding about the molecular characterization of the Egyptian APV strains.

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References

  1. 1.

    C.L. Afonso, E.R. Tulman, Z. Lu, L. Zsak, G.F. Kutish, D.L. Rock, The genome of fowlpox virus. J. Virol. 74, 3815–3831 (2000)

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    M.M. Amer, A.M.W. Kheir Eldin, M.H.H. Awaad, Studies on pox in Japanese quail (Coturnix Coturnix Japonica). J. Egypt. Vet. Med. Assoc. 46, 295–302 (1986)

    Google Scholar 

  3. 3.

    S.K. Biswas, C. Jana, K. Chand, W. Rehman, B. Mondal, Detection of fowl poxvirus integrated with reticuloendotheliosis virus sequences from an outbreak in backyard chickens in India. Vet. Ital. 47, 147–153 (2011)

    PubMed  Google Scholar 

  4. 4.

    A. Bolte, J. Meurer, E. Kaleta, Avian host spectrum of avipoxviruses. Avian Pathol. 28, 415–432 (1999)

    Article  Google Scholar 

  5. 5.

    O. Carulei, N. Douglass, A.L. Williamson, Phylogenetic analysis of three genes of Penguinpox virus corresponding to Vaccinia virus G8R (VLTF-1), A3L (P4b) and H3L reveals that it is most closely related to Turkeypox virus, Ostrichpox virus and Pigeonpox virus. Virol. J. 6, 52 (2009)

    PubMed  Article  Google Scholar 

  6. 6.

    A.A.M. Eid, M.A. EL-Said, I.A. Ghanem, Pox Virus Infection Among Some Avian Species In Sharkia Province (4th Veterinary Medicine Zagazig Congress, Hurghada, 1998), pp. 681–688

    Google Scholar 

  7. 7.

    K.S. EL-Zanaty, Identification and characterization of pox virus isolated from turkey. Assiut Vet. Med. J. 23, 240–248 (1990)

    Google Scholar 

  8. 8.

    C. Gubser, S. Hue, P. Kellam, G.L. Smith, Poxvirus genomes: a phylogenetic analysis. J. Gen. Virol. 85, 105–117 (2004)

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    H.J. Ha, L. Howe, M. Alley, B. Gartrell, The phylogenetic analysis of avipoxvirus in New Zealand. Vet. Microbiol. 150, 80–87 (2011)

    PubMed  Article  Google Scholar 

  10. 10.

    L. Huw Lee, K. Hwa Lee, Application of the polymerase chain reaction for the diagnosis of fowl poxvirus infection. J. Virol. Methods 63, 113–119 (1997)

    PubMed  Article  CAS  Google Scholar 

  11. 11.

    S. Jarmin, R. Manvell, R.E. Gough, S.M. Laidlaw, M.A. Skinner, Avipoxvirus phylogenetics: identification of a PCR length polymorphism that discriminates between the two major clades. J. Gen. Virol. 87, 2191–2201 (2006)

    PubMed  Article  CAS  Google Scholar 

  12. 12.

    D. Luschow, T. Hoffmann, H.M. Hafez, Differentiation of avian poxvirus strains on the basis of nucleotide sequences of 4b gene fragment. Avian Dis. 48, 453–462 (2004)

    PubMed  Article  CAS  Google Scholar 

  13. 13.

    G. Manarolla, G. Pisoni, G. Sironi, T. Rampin, Molecular biological characterization of avian poxvirus strains isolated from different avian species. Vet. Microbiol. 140, 1–8 (2009)

    PubMed  Article  Google Scholar 

  14. 14.

    M. Mohan, T.F. Fernandez, A case report of Pigeon pox-histopathologic diagnosis. Vet. World 1, 117–118 (2008)

    Google Scholar 

  15. 15.

    R.M. Pawar, S.S. Bhushan, A. Poornachandar, U. Lakshmikantan, S. Shivaji, Avian pox infection in different wild birds in India. Eur. J. Wildl. Res. 57, 785–793 (2011)

    Article  Google Scholar 

  16. 16.

    J. Perez-Tris, R.A. Williams, E. Abel-Fernandez, J. Barreiro, J.J. Conesa, J. Figuerola, M. Martinez–Martinez, A. Ramirez, L. Benitez, A multiplex PCR for detection of poxvirus and papillomavirus in cutaneous warts from live birds and museum skins. Avian Dis. 55, 545–553 (2011)

    PubMed  Article  CAS  Google Scholar 

  17. 17.

    M. Saif-Edin, S.S. El-Ballal, Epidemiological and ultrastructural studies on Pigeon pox in upper Egypt. Assiut Vet. Med. J. 37, 68–85 (1997)

    Google Scholar 

  18. 18.

    H.L. Shivaprasad, T. Kim, D. Tripathy, P.R. Woolcock, F. Uzal, Unusual pathology of canary poxvirus infection associated with high mortality in young and adult breeder canaries (Serinus canaria). Avian Pathol. 38, 311–316 (2009)

    PubMed  Article  CAS  Google Scholar 

  19. 19.

    D.N. Tripathy, W.M. Reed, Pox, in Diseases of Poultry, 11th edn., ed. by Y.M. Saif, H.J. Barnes, J.R. Glisson, A.M. Fadly, L.R. McDougald, D.E. Swayne (Iowa State University Press, Ames, 2003), pp. 253–269

    Google Scholar 

  20. 20.

    S.C. Weli, M.I. Okeke, M. Tryland, O. Nilssen, T. Traavik, Characterization of avipoxviruses from wild birds in Norway. Can. J. Vet. Res. 68, 140–145 (2004)

    PubMed  Google Scholar 

  21. 21.

    S.C. Weli, T. Traavik, M. Tryland, D.H. Coucheron, O. Nilssen, Analysis and comparison of the 4b core protein gene of avipoxviruses from wild birds: evidence for interspecies spatial phylogenetic variation. Arch. Virol. 149, 2035–2046 (2004)

    PubMed  CAS  Google Scholar 

  22. 22.

    S.C. Weli, M. Tryland, Avipoxviruses: infection biology and their use as vaccine vectors. Virol. J. 8, 49 (2011)

    PubMed  Article  CAS  Google Scholar 

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Acknowledgments

We are thankful to Prof Dr Abd El-Moneim Ahmed Ali, Dr Haitham Alyadin, and Mostafa Hassan (Zagazig University) for histopathologic examination and imaging. Ahmed El-Baqer for PGPV clinical sample provision.

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Correspondence to Fatma M. Abdallah.

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Abdallah, F.M., Hassanin, O. Detection and molecular characterization of avipoxviruses isolated from different avian species in Egypt. Virus Genes 46, 63–70 (2013). https://doi.org/10.1007/s11262-012-0821-y

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Keywords

  • Avipoxvirus
  • Elsharqyia_FWPV
  • Elsharqyia_TKPV
  • Elsharqyia_PGPV
  • P4b
  • fpv167
  • fpv140
  • Egypt
  • Backyard