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Molecular characterization of Japanese encephalitis viruses circulating in pigs and mosquitoes on pig farms in the Chinese province of Henan

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Abstract

Since the first Chinese case report of Japanese encephalitis, Japanese encephalitis virus (JEV) has circulated in China for at least 60 years. Even though pigs play a critical role in the JEV transmission cycle information on the prevalence of JEV in pigs has not been investigated in China. As the central Chinese province of Henan has the largest human population in China, a history of serious JEV and is the largest pig producing province it was chosen for this study. We have found that currently natural infection with JEV in pigs and mosquitoes is prevalent and both genotypes 1 and 3 co-circulate in pigs and mosquitoes in central China. Phylogenetic analysis showed that all of the newly obtained pig-derived JEV isolates are more closely related to isolates from the 1950s to 1960s than to those recently isolated from humans and mosquitoes. Further analyses based on all the previous reported Chinese isolates indicates that presently genotype 3 JEV is the predominant genotype in pigs but genotype 1 JEV is emerging and spreading rapidly in recent years. Our study provides information for understanding the current epidemiology of JEV in China and suggests possible measures applicable to the further control of JEV.

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Acknowledgments

This work was supported by the Special Funds for Agro-scientific Research in the Public Interest (Grant No. 201203082-5). The authors also gratefully acknowledge the critical review by Prof. Norman A. Gregson (ION, UCL, London, UK).

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Authors and Affiliations

  1. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People’s Republic of China

    Man Teng, Jun Luo, Xing-Tao Wang & Gai-Ping Zhang

  2. The Laboratory of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Jian-Ming Fan & Wu Yao

  3. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Lu Chen & Chuan-Qing Wang

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  1. Man Teng
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  2. Jun Luo
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  3. Jian-Ming Fan
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  4. Lu Chen
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  7. Chuan-Qing Wang
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Correspondence to Jun Luo or Gai-Ping Zhang.

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Teng, M., Luo, J., Fan, JM. et al. Molecular characterization of Japanese encephalitis viruses circulating in pigs and mosquitoes on pig farms in the Chinese province of Henan. Virus Genes 46, 170–174 (2013). https://doi.org/10.1007/s11262-012-0813-y

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  • DOI: https://doi.org/10.1007/s11262-012-0813-y

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