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Analysis of EpapGV gp37 gene reveals a close relationship between granulovirus and entomopoxvirus

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Abstract

The Epinotia aporema Granulovirus GP37 protein gene has been identified, located, and sequenced. This gene was similar to other baculovirus gp37, to entomopoxvirus fusolin gene, and to the chitin-binding protein gene of bacteria. Sequence analysis indicated that the open reading frame is 669 bp long (the smallest gp37 sequenced at present) and encodes a predicted 222-amino acid protein. This protein is glycosylated and specifically recognized by an entomopoxvirus fusolin antiserum. The pairwise comparison of EpapGV gp37 gene product with all the baculovirus sequences in GenBank yields high similarity values ranging from 45 to 63 % with Cydia pomonella Granulovirus gp37 being the most closely related. The phylogenetic analysis interestingly grouped the granuloviruses in a cluster more closely related to entomopoxviruses than to nucleopolyhedroviruses, suggesting a possible horizontal transfer event between the granulovirus group and the entomopoxvirus group.

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Acknowledgments

The authors thank Mrs. M. Cornacchio and Mrs. D.B. Moreyra for the maintenance of the insect colony at the INTA. This work was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) and the Instituto Nacional de Tecnología Agropecuaria (INTA).

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Author notes

  1. Marina E. Biedma

    Present address: INSERM U748, Institut de Virologie, Faculté de Médecine, Université de Strasbourg, 3 rue Koeberlé, 67000, Strasbourg, France

Authors and Affiliations

  1. Instituto de Microbiología y Zoología Agrícola (IMYZA-CICVyA-INTA), CC 25 (B1712WAA), Castelar, Buenos Aires, Argentina

    Ricardo Salvador, Marcelo F. Berretta & Alicia Sciocco-Cap

  2. Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Argentina

    Ricardo Salvador, M. Leticia Ferrelli, Marina E. Biedma & Víctor Romanowski

  3. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8634, Japan

    Wataru Mitsuhashi

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  1. Ricardo Salvador
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Correspondence to Ricardo Salvador.

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Ricardo Salvador and M. Leticia Ferrelli are contributed equally to this study.

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Salvador, R., Ferrelli, M.L., Berretta, M.F. et al. Analysis of EpapGV gp37 gene reveals a close relationship between granulovirus and entomopoxvirus. Virus Genes 45, 610–613 (2012). https://doi.org/10.1007/s11262-012-0800-3

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  • DOI: https://doi.org/10.1007/s11262-012-0800-3

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