Skip to main content

Identification and phylogenetic analysis of orf viruses isolated from outbreaks in goats of Assam, a northeastern state of India

Virus Genes volume 45, pages 98–104 (2012)Cite this article

Abstract

Two outbreaks of orf virus (ORFV) (a parapoxvirus) infection in goats, which occurred in Golaghat and Kamrup districts of Assam, a northeastern part of India, were investigated. The disease was diagnosed by standard virological and molecular techniques. The entire protein-coding region of B2L gene of two isolates were cloned and sequenced. Phylogenetic analysis based on B2L amino acid sequences showed that the ORFVs identified in these outbreaks were closely related to each other and both were closer to ORFV-Shahjahanpur 82/04 isolate from north India. The present study revealed that the precise characterization of the genomic region (B2L gene) might provide evidence for the genetic variation and movement of circulating ORFV strains in India.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  1. M. Hosamani, A. Scagliarini, V. Bhanuprakash, C.J. McInnes, R.K. Singh, Expert. Rev. Anti. Infect. Ther. 7, 879 (2009)

    PubMed  Article  Google Scholar 

  2. K. Zhao, D. Song, W. He, H. Lu, B. Zhang, C. Li, K. Chen, F. Gao, Vet. Microbiol. 142, 408 (2010)

    PubMed  Article  CAS  Google Scholar 

  3. P. Saravanan, V. Balamurugan, A. Sen, J. Sarkar, B. Sahay, K.K. Rajak, M. Hosamani, M.P. Yadav, R.K. Singh, Vet. Rec. 160, 410 (2007)

    PubMed  Article  CAS  Google Scholar 

  4. G. Delhon, E.R. Tulman, C.L. Afonso, Z. Lu, A. de la Concha-Bermejillo, H.D. Lehmkuhl, M.E. Piccone, G.F. Kutish, D.L. Rock, J. Virol. 78, 168 (2004)

    PubMed  Article  CAS  Google Scholar 

  5. Y. Inoshima, A. Morooka, H. Sentsui, J. Virol. Methods 84, 201 (2000)

    PubMed  Article  CAS  Google Scholar 

  6. D.P. Bora, G. Venkatesan, V. Bhanuprakash, V. Balamurugan, M. Prabhu, M.S. Siva Sankar, R. Yogisharadhya, J. Virol. Methods 178, 249 (2011)

    PubMed  Article  CAS  Google Scholar 

  7. M. Hosamani, V. Bhanuprakash, A. Scagliarini, R.K. Singh, Vet. Microbiol. 116, 317 (2006)

    PubMed  Article  CAS  Google Scholar 

  8. B. Mondal, A.K. Bera, M. Hosamani, P.A. Tembhurne, S.K. Bandyopadhyay, Vet. Res. Commun. 30, 531 (2006)

    PubMed  Article  CAS  Google Scholar 

  9. M. Hosamani, S. Yadav, D.J. Kallesh, B. Mondal, V. Bhanuprakash, R.K. Singh, Zoonoses Public Health 54, 204 (2007)

    PubMed  Article  CAS  Google Scholar 

  10. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731 (2011)

    PubMed  Article  CAS  Google Scholar 

  11. G. Venkatesan, V. Balamurugan, D.P. Bora, R. Yogisharadhya, M. Prabhu, V. Bhanuprakash, Vet. Ital. 43, 323 (2011)

    Google Scholar 

  12. J.S. Abrahão, R.K. Campos, G.S. Trindade, M.I. Guedes, Z.I. Lobato, C. Mazur, P.C. Ferreira, C.A. Bonjardim, E.G. Kroon, Virol. J. 6, 47 (2009)

    PubMed  Article  Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Director, Indian Veterinary Research Institute (IVRI), Izatnagar, and the Head, Division of Virology, IVRI, Mukteswar, Uttarakhand, for providing the necessary facilities. The authors thank the Head of Department, Microbiology, College of Veterinary Science, Khanapara, and field veterinarians for their kind cooperation in providing clinical samples, photographs, and epidemiological information of contagious ecthyma outbreaks.

Author information

Authors and Affiliations

  1. Pox Virus Laboratory, Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Nainital District, Kumaon, 263 138, Uttarakhand, India

    Durlav Prasad Bora, Veerakyathappa Bhanuprakash, Revanaiah Yogisharadhya, Gnanavel Venkatesan & Amit Kumar

  2. Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara Campus, Guwahati, 781 022, Assam, India

    Durlav Prasad Bora, Nagendra Nath Barman, Sailendra Kumar Das, Gitika Rajbongshi & Elina Khatoon

  3. AAU, Khanapara, Guwahati, Assam, India

    Apurba Chakraborty

  4. Assam Agriculture University, Jorhat, Assam, India

    Kamal Malla Bujarbaruah

Authors
  1. Durlav Prasad Bora
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nagendra Nath Barman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sailendra Kumar Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Veerakyathappa Bhanuprakash
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Revanaiah Yogisharadhya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gnanavel Venkatesan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Amit Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Gitika Rajbongshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Elina Khatoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Apurba Chakraborty
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Kamal Malla Bujarbaruah
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Durlav Prasad Bora.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bora, D.P., Barman, N.N., Das, S.K. et al. Identification and phylogenetic analysis of orf viruses isolated from outbreaks in goats of Assam, a northeastern state of India. Virus Genes 45, 98–104 (2012). https://doi.org/10.1007/s11262-012-0740-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-012-0740-y

Keywords

  • B2L gene
  • Contagious ecthyma
  • Goat
  • Orf virus
  • Phylogenetic analysis
  • Northeast India