Virus Genes

, Volume 44, Issue 3, pp 513–521 | Cite as

Analysis of two strains of Peanut stunt virus: satRNA-associated and satRNA free

  • Aleksandra Obrępalska-Stęplowska
  • Marta Budziszewska
  • Przemysław Wieczorek
  • Anna Czerwoniec
Article

Abstract

Peanut stunt virus (PSV) is a pathogen of legumes, vegetables, trees, and weeds occurring worldwide. The species is characterized by significant genetic variability. PSV strains are classified into four subgroups on the basis of their nucleotide sequence homology. Here, we are presenting two further, fully sequenced PSV strains—PSV-Ag and PSV-G, that could be considered as I subgroup representatives. However, their sequence homology with other typical I subgroups members, similarly as another strain—PSV-P, characterized by our group previously, is lower than 90%. This lead us to propose further subdivision of the I subgroup into IA, IB, and IC units, and to classify PSV-Ag and PSV-G strains to the last one. In this article, we are showing that identity level of PSV-Ag and PSV-G is very high and apart from the presence of satRNA in the first one, they differ only by a few nucleotides in their genomic RNAs. Nevertheless, symptoms they cause on host plants might differ significantly, just as the levels in infected plants. Effect of single amino acid changes between strains on the three-dimensional structure of viral proteins was analyzed. Differences occur mainly on the protein surfaces which can possibly affect protein–protein interaction in infected cells, which is discussed.

Keywords

PSV Classification satRNA Symptom expression Three-dimensional model structure of viral proteins 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Aleksandra Obrępalska-Stęplowska
    • 1
  • Marta Budziszewska
    • 1
  • Przemysław Wieczorek
    • 1
  • Anna Czerwoniec
    • 2
  1. 1.Interdepartmental Laboratory of Molecular BiologyInstitute of Plant Protection—National Research InstitutePoznanPoland
  2. 2.Bioinformatics Laboratory, Institute of Molecular Biology and BiotechnologyAdam Mickiewicz UniversityPoznanPoland

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