Abstract
Equine herpesvirus 4 (EHV-4) is an important pathogen that causes respiratory tract disease in horse populations worldwide. Glycoprotein G (gG) homologs have been identified in several alphaherpesviruses as minor non-essential membrane-anchored glycoproteins. In this study, EHV-4 gG deletion mutant has been generated by using bacterial artificial chromosome technology to investigate the role of gG in viral pathogenesis. Our findings reported here revealed no significant difference between parental EHV-4 and gG-negative strain in their replication cycle in cell culture. Furthermore, virus titers and plaque formation were comparable in both viruses. It is noteworthy that these findings disagree with the previously published study describing gG deletion in another EHV-4 strain.
References
A.J. Davison, R. Eberle, B. Ehlers, G.S. Hayward, D.J. McGeoch, A.C. Minson, P.E. Pellett, B. Roizman, M.J. Studdert, E. Thiry, Arch. Virol. 154, 171–177 (2009)
B. Roizman, Herpesviridae, in Virology, ed. by B.N. Field, D.M. Knipe, P.M. Howley, R.M. Channock, J.L. Melnick, T.P. Monath, B. Roizman, S.E. Straus (Lippincott-Raven, Philadelphia, 1996)
C.F. Colle 3rd, C.C. Flowers, D.J. O’Callaghan, Virology 188, 545–557 (1992)
B.S. Crabb, H.S. Nagesha, M.J. Studdert, Virology 190, 143–154 (1992)
R. Eberle, M. Zhang, D.H. Black, Arch. Virol. 130, 391–411 (1993)
T. Engelhardt, G.M. Keil, Virology 225, 126–135 (1996)
N. Ficorilli, M.J. Studdert, B.S. Crabb, Arch. Virol. 140, 1653–1662 (1995)
H. Fukushi, T. Tomita, A. Taniguchi, Y. Ochiai, R. Kirisawa, T. Matsumura, T. Yanai, T. Masegi, T. Yamaguchi, K. Hirai, Virology 227, 34–44 (1997)
D.J. McGeoch, H.W. Moss, D. McNab, M.C. Frame, J. Gen. Virol. 68(Pt 1), 19–38 (1987)
T.J. Rea, J.G. Timmins, G.W. Long, L.E. Post, J. Virol. 54, 21–29 (1985)
M.J. Slomka, L. Harrington, C. Arnold, J.P. Norcott, D.W. Brown, J. Gen. Virol. 76(Pt 9), 2161–2168 (1995)
S.J. Spatz, P.A. Rota, R.K. Maes, J. Gen. Virol. 75(Pt 6), 1235–1244 (1994)
H.E. Drummer, M.J. Studdert, B.S. Crabb, J. Gen. Virol. 79(Pt 5), 1205–1213 (1998)
W. Azab, K. Kato, J. Arii, K. Tsujimura, D. Yamane, Y. Tohya, T. Matsumura, H. Akashi, Arch. Virol. 154, 833–842 (2009)
E.C. Lee, D. Yu, J. Martinez de Velasco, L. Tessarollo, D.A. Swing, D.L. Court, N.A. Jenkins, N.G. Copeland, Genomics 73, 56–65 (2001)
B.K. Tischer, J. von Einem, B. Kaufer, N. Osterrieder, Biotechniques 40, 191–197 (2006)
J. Huang, C.A. Hartley, N.P. Ficorilli, B.S. Crabb, M.J. Studdert, Arch. Virol. 150, 2583–2592 (2005)
Y. Sun, A.R. MacLean, J.D. Aitken, S.M. Brown, J. Gen. Virol. 77(Pt 3), 493–500 (1996)
K. Maeda, K. Kai, T. Matsumura, J. Vet. Med. Sci. 67, 555–561 (2005)
H.S. Nagesha, J.R. McNeil, N. Ficorilli, M.J. Studdert, Arch. Virol. 124, 379–387 (1992)
N.A. Bryant, N. Davis-Poynter, A. Vanderplasschen, A. Alcami, EMBO J. 22, 833–846 (2003)
G.R. Van de Walle, M.L. May, W. Sukhumavasi, J. von Einem, N. Osterrieder, J. Immunol. 179, 4161–4169 (2007)
G.R. Van de Walle, K. Sakamoto, N. Osterrieder, J. Virol. 82, 1714–1722 (2008)
E.A. Telford, M.S. Watson, J. Perry, A.A. Cullinane, A.J. Davison, J. Gen. Virol. 79, 1197–1203 (1998)
Acknowledgments
This study was supported in part by grants from the Egyptian Ministry of Higher Education and a Grant-in-Aid for Scientific Research in Priority Areas from the Ministry of Education, Culture, Science, Sports and Technology (MEXT) of Japan.