Analysis of the sequence of a dicot-infecting mastrevirus (family Geminiviridae) originating from Syria

Abstract

Chickpea stunt disease (CSD) across southern Asia, the Middle East and North Africa is caused by a number of viruses that include single-stranded DNA viruses of the genus Mastrevirus (family Geminiviridae). Despite the importance of CSD in reducing chickpea and lentil production, until recently little was known of the nature of the pathogens causing the disease. Sequence characterisation of virus isolates from Sudan and Pakistan showed the viruses concerned to potentially be new mastrevirus species related to Bean yellow dwarf virus (BeYDV), a virus known to occur in both southern Africa and southern Asia. Here we have determined the complete nucleotide sequence of a mastrevirus associated with CSD in Syria. This virus represents a proposed new species, closely related to the recently characterised Chickpea chlorotic dwarf Sudan virus and Chickpea chlorotic dwarf Pakistan virus but with the highest sequence identity to BeYDV, for which we propose the name Chickpea chlorotic dwarf Syria virus. In addition the biological integrity of the clone was confirmed by infection of Nicotiana benthamiana plants using Agrobacterium-mediated inoculation.

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Acknowledgments

H.M. was supported by the Higher Education Commission (HEC, Pakistan) under the ‘Indigenous 5000 Fellowship Scheme’. The authors are grateful to Dr. M. Shafiq Shahid for his help in the production of constructs for agroinoculation. R.W.B. was supported by the HEC under the ‘Foreign Faculty Scheme’. Funding for this research came, in part, from an ICGEB-TWAS-UNESCO/IBSP grant under the “Joint Project on Capacity Building in Basic Molecular Biology” scheme. The support of NIBGE in conducting this research is gratefully acknowledged.

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Correspondence to Rob W. Briddon.

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Mumtaz, H., Kumari, S.G., Mansoor, S. et al. Analysis of the sequence of a dicot-infecting mastrevirus (family Geminiviridae) originating from Syria. Virus Genes 42, 422–428 (2011). https://doi.org/10.1007/s11262-011-0586-8

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Keywords

  • Chickpea stunt disease
  • Mastrevirus
  • Geminivirus