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Genetic reassortment between high-virulent and low-virulent Dobrava-Belgrade virus strains

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Abstract

The tri-segmented RNA genome of hantaviruses facilitates genetic reassortment by segment swapping when cells are co-infected with different virus strains. We found efficient in vitro reassortment between members of two different genetic lineages of the Dobrava-Belgrade virus species, the weakly virulent DOBV-Aa and highly virulent DOBV-Af. In all reassortants, S and L segments originated from the same parental strain, and only the M segment was exchanged. To identify functional differences between the parental strains DOBV-Aa and DOBV-Af in cell culture and to compare them with the reassortants, we studied elements of the innate immunity in virus-infected cells. The contrasting phenotypes of the parental viruses were maintained by the reassortants carrying the respective S and L segments of the parental virus and were not influenced by the origin of the M segment.

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References

  1. N.N. Zhou, D.A. Senne, J.S. Landgraf, S.L. Swenson, G. Erickson, K. Rossow, L. Liu, K. Yoon, S. Krauss, R.G. Webster, J. Virol. 73, 8851–8856 (1999)

    CAS  PubMed  Google Scholar 

  2. A.A. Sall, P.M. Zanotto, O.K. Sene, H.G. Zeller, J.P. Digoutte, Y. Thiongane, M. Bouloy, J. Virol. 73, 8196–8200 (1999)

    CAS  PubMed  Google Scholar 

  3. S.R. Gerrard, L. Li, A.D. Barrett, S.T. Nichol, J. Virol. 78, 8922–8926 (2004)

    Article  CAS  PubMed  Google Scholar 

  4. T. Briese, V. Kapoor, W.I. Lipkin, Arch. Virol. 152, 2237–2247 (2007)

    Article  CAS  PubMed  Google Scholar 

  5. S.M. Reese, B.J. Blitvich, C.D. Blair, D. Geske, B.J. Beaty, W.C. Black IV, Virol. J. 5, 164 (2008)

    Article  PubMed  Google Scholar 

  6. C. Schmaljohn, B. Hjelle, Emerg. Infect. Dis. 3, 95–104 (1997)

    Article  CAS  PubMed  Google Scholar 

  7. D.H. Kruger, R. Ulrich, A. Lundkvist, Microbes Infect. 3, 1129–1144 (2001)

    Article  CAS  PubMed  Google Scholar 

  8. P. Maes, J. Clement, I. Gavrilovskaya, M. van Ranst, Viral Immunol. 17, 481–497 (2004)

    Article  CAS  PubMed  Google Scholar 

  9. P. Maes, B. Klempa, J. Clement, J. Matthijnssens, D.C. Gajdusek, D.H. Kruger, M. van Ranst, Infect. Genet. Evol. 9, 813–820 (2009)

    Article  CAS  PubMed  Google Scholar 

  10. D. Li, A.L. Schmaljohn, K. Anderson, C.S. Schmaljohn, Virology 206, 973–983 (1995)

    Article  CAS  PubMed  Google Scholar 

  11. W.W. Henderson, M.C. Monroe, S.C. St. Jeor, W.P. Thayer, J.E. Rowe, C.J. Peters, S.T. Nichol, Virology 214, 602–610 (1995)

    Article  CAS  PubMed  Google Scholar 

  12. B. Klempa, H.A. Schmidt, R. Ulrich, S. Kaluz, M. Labuda, H. Meisel, B. Hjelle, D.H. Kruger, J. Virol. 77, 804–809 (2003)

    Article  CAS  PubMed  Google Scholar 

  13. Y. Zou, J. Hu, Z.X. Wang, D.M. Wang, C. Yu, J.Z. Zhou, Z.F. Fu, Y.Z. Zhang, J. Med. Virol. 80, 1033–1041 (2008)

    Article  PubMed  Google Scholar 

  14. M. Razzauti, A. Plyusnina, H. Henttonen, A. Plyusnin, J. Gen. Virol. 89, 1649–1660 (2008)

    Article  CAS  PubMed  Google Scholar 

  15. M. Razzauti, A. Plyusnina, T. Sironen, H. Henttonen, A. Plyusnin, J. Gen. Virol. 90, 1923–1931 (2009)

    Article  CAS  PubMed  Google Scholar 

  16. S.T. Nichol, B.J. Beaty, R.M. Elliott, R. Goldbach, A. Plyusnin, C.S. Schmaljohn, R.B. Tesh. Family Bunyaviridae, in Virus Taxonomy: VIII Report of the International Committee on Taxonomy of Viruses, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Elsevier, London, 2005) pp. 695–716

  17. L.L. Rodriguez, J.H. Owens, C.J. Peters, S.T. Nichol, Virology 242, 99–106 (1998)

    Article  CAS  PubMed  Google Scholar 

  18. A.A. Rizvanov, S.F. Khaiboullina, S. St. Jeor, Virology 327, 225–232 (2004)

    Article  CAS  PubMed  Google Scholar 

  19. A.K. McElroy, J.M. Smith, J.W. Hooper, C.S. Schmaljohn, Virology 326, 130–139 (2004)

    Article  CAS  PubMed  Google Scholar 

  20. W. Handke, R. Oelschlegel, R. Franke, L. Wiedemann, D.H. Krüger, A. Rang, J. Gen. Virol. 91 (2010)

  21. G. Schönrich, A. Rang, N. Lütteke, M.J. Raftery, N. Charbonnel, R.G. Ulrich, Immunol. Rev. 225, 481–497 (2008)

    Article  Google Scholar 

  22. H. Ebihara, K. Yoshimatsu, M. Ogino, K. Araki, Y. Ami, H. Kariwa, I. Takashima, D. Li, J. Arikawa, J. Virol. 74, 9245–9255 (2000)

    Article  CAS  PubMed  Google Scholar 

  23. T. Avsic-Zupanc, S.Y. Xiao, R. Stojanovic, A. Gligic, G. van der Groen, J.W. LeDuc, J. Med. Virol. 38, 132–137 (1992)

    Article  CAS  PubMed  Google Scholar 

  24. A. Gligic, N. Dimkovic, S.Y. Xiao, G.J. Buckle, D. Jovanovic, D. Velimirovic, R. Stojanovic, M. Obradovic, G. Diglisic, J. Micic et al., J. Infect. Dis. 166, 113–120 (1992)

    CAS  PubMed  Google Scholar 

  25. K. Nemirov, O. Vapalahti, A. Lundkvist, V. Vasilenko, I. Golovljova, A. Plyusnina, J. Niemimaa, J. Laakkonen, H. Henttonen, A. Vaheri, A. Plyusnin, J. Gen. Virol. 80, 371–379 (1999)

    CAS  PubMed  Google Scholar 

  26. B. Klempa, M. Stanko, M. Labuda, R. Ulrich, H. Meisel, D.H. Kruger, J. Clin. Microbiol. 43, 2756–2763 (2005)

    Article  PubMed  Google Scholar 

  27. H. Henttonen, P. Buchy, Y. Suputtamongkol, S. Jittapalapong, V. Herbreteau, J. Laakkonen, Y. Chaval, M. Galan, G. Dobigny, N. Charbonnel, J. Michaux, J.F. Cosson, S. Morand, J.P. Hugot, Ann. NY Acad. Sci. 1149, 84–89 (2008)

    Article  CAS  PubMed  Google Scholar 

  28. T. Sironen, A. Vaheri, A. Plyusnin, Virol. J. 2, 90 (2005)

    Article  PubMed  Google Scholar 

  29. T. Avsic-Zupanc, M. Petrovec, P. Furlan, R. Kaps, F. Elgh, A. Lundkvist, Clin. Infect. Dis. 28, 860–865 (1999)

    Article  CAS  PubMed  Google Scholar 

  30. A. Papa, A. Antoniadis, Eur. J. Epidemiol. 17, 189–194 (1999)

    Article  Google Scholar 

  31. B. Klempa, M. Schutt, B. Auste, M. Labuda, R. Ulrich, H. Meisel, D.H. Kruger, J. Clin. Microbiol. 42, 1322–1325 (2004)

    Article  CAS  PubMed  Google Scholar 

  32. B. Klempa, E.A. Tkachenko, T.K. Dzagurova, Y.V. Yunicheva, V.G. Morozov, N.M. Okulova, G.P. Slyusareva, A. Smirnov, D.H. Kruger, Emerg. Infect. Dis. 14, 617–625 (2008)

    Article  PubMed  Google Scholar 

  33. T.K. Dzagurova, B. Klempa, E.A. Tkachenko, G.P. Slyusareva, V.G. Morozov, B. Auste, D.H. Kruger, J. Clin. Microbiol. 47, 4029–4036 (2009)

    Article  CAS  PubMed  Google Scholar 

  34. H. Heider, B. Ziaja, C. Priemer, A. Lundkvist, J. Neyts, D.H. Krüger, R. Ulrich, J. Virol. Methods 96, 17–23 (2001)

    Article  CAS  PubMed  Google Scholar 

  35. A. Rang, H. Heider, R. Ulrich, D.H. Krüger, J. Virol. Methods 135, 26–31 (2006)

    Article  CAS  PubMed  Google Scholar 

  36. S.F. Emonet, G. Grard, N.M. Brisbarre, G.N. Moureau, S. Temmam, R.N. Charrel, X. de Lamballerie, Nat. Protoc. 2, 340–346 (2007)

    Article  CAS  PubMed  Google Scholar 

  37. T.A. Hall, Nucl. Acids Symp. Ser. 41, 95–98 (1999)

    CAS  Google Scholar 

  38. K.S. Lole, R.C. Bollinger, R.S. Paranjape, D. Gadkari, S.S. Kulkarni, N.G. Novak, R. Ingersoll, H.W. Sheppard, S.C. Ray, J. Virol. 73, 152–160 (1999)

    CAS  PubMed  Google Scholar 

  39. H.A. Schmidt, K. Strimmer, M. Vingron, A. von Haeseler, Bioinformatics 18, 502–504 (2002)

    Article  CAS  PubMed  Google Scholar 

  40. W. Handke, R. Oelschlegel, R. Franke, D.H. Krüger, A. Rang, J. Immunol. 182, 2849–2858 (2009)

    Article  CAS  PubMed  Google Scholar 

  41. F. Flohr, S. Schneider-Schaulies, O. Haller, G. Kochs, FEBS Lett. 463, 24–28 (1999)

    Article  CAS  PubMed  Google Scholar 

  42. M. Schlegel, B. Klempa, B. Auste, M. Bemmann, J. Schmidt-Chanasit, T. Büchner, M.H. Groschup, M. Meier, A. Buschmann, H. Zoller, D.H. Kruger, R.G. Ulrich, Emerg. Infect. Dis. 15, 2017–2020 (2009)

    Article  PubMed  Google Scholar 

  43. M.A. Mir, S. Sheema, A. Haseeb, A. Haque, J. Biol. Chem. 285, 11357–11368 (2010)

    Article  CAS  PubMed  Google Scholar 

  44. D.H. Kruger, B. Klempa, Dobrava virus, in Molecular detection of human viral pathogens, ed. by D. Liu (CRC Press, Boca Raton, FL, 2010)

  45. E. Geimonen, S. Neff, T. Raymond, S.S. Kocer, I.N. Gavrilovskaya, E.R. Mackow, Proc. Natl. Acad. Sci. USA 99, 13837–13842 (2002)

    Article  CAS  PubMed  Google Scholar 

  46. A.A. Kraus, M.J. Raftery, T. Giese, R. Ulrich, R. Zawatzky, S. Hippenstiel, N. Suttorp, D.H. Kruger, G. Schonrich, J. Virol. 78, 6143–6150 (2004)

    Article  CAS  PubMed  Google Scholar 

  47. P.J. Alff, I.N. Gavrilovskaya, E. Gorbunova, K. Endriss, Y. Chong, E. Geimonen, N. Sen, N.C. Reich, E.R. Mackow, J. Virol. 80, 9676–9686 (2006), Erratum in: J. Virol. 80, 12430 (2006)

    Google Scholar 

  48. C.F. Spiropoulou, C.G. Albariño, T.G. Ksiazek, P.E. Rollin, J. Virol. 81, 2769–2776 (2007)

    Article  CAS  PubMed  Google Scholar 

  49. P.J. Alff, N. Sen, E. Gorbunova, I.N. Gavrilovskaya, E.R. Mackow, J. Virol. 82, 9115–9122 (2008)

    Article  CAS  PubMed  Google Scholar 

  50. M. Habjan, A. Pichlmair, R.M. Elliott, A.K. Overby, T. Glatter, M. Gstaiger, G. Superti-Furga, H. Unger, F. Weber, J. Virol. 83, 4365–4375 (2009)

    Article  CAS  PubMed  Google Scholar 

  51. T. Ikegami, K. Narayanan, S. Won, W. Kamitani, C.J. Peters, S. Makino, PLoS Pathog. 5, e1000287 (2009)

    Article  PubMed  Google Scholar 

  52. S.L. Taylor, N. Frias-Staheli, A. García-Sastre, C.S. Schmaljohn, J. Virol. 83, 1271–1279 (2009)

    Article  CAS  PubMed  Google Scholar 

  53. S.L. Taylor, R.L. Krempel, C.S. Schmaljohn, Ann. NY Acad. Sci. 1171 (Suppl 1), E86–E93 (2009)

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Acknowledgments

We thank Dr. Martin Raftery for critical reading of the manuscript. SK and MHL were graduates funded by Deutsche Forschungsgemeinschaft (DFG) in the Research Training group 1121. Parts of the work were supported by additional DFG grants (KR1293/9-1 and RA1057/2-1).

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Correspondence to Detlev H. Kruger.

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Kirsanovs, S., Klempa, B., Franke, R. et al. Genetic reassortment between high-virulent and low-virulent Dobrava-Belgrade virus strains. Virus Genes 41, 319–328 (2010). https://doi.org/10.1007/s11262-010-0523-2

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  • DOI: https://doi.org/10.1007/s11262-010-0523-2

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