Skip to main content
SpringerLink
Log in

Functional analysis of Spodoptera litura nucleopolyhedrovirus p49 gene during Autographa californica nucleopolyhedrovirus infection of SpLi-221 cells

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

The Spodoptera litura nucleopolyhedrovirus (SpltNPV) antiapoptotic gene Splt-p49 is able to rescue replication of a p35-null Autographa californica nucleopolyhedrovirus (AcMNPV) in AcMNPV-permissive Sf9 cells. In this study, an AcMNPV p35 knockout bacmid was generated through ET homologous recombination in Escherichia coli and designated as vAcP35-KO. The Splt-p49 gene was transposed into the polyhedrin locus of vAcP35-KO to investigate if Splt-p49 has any antiapoptotic activity in the context of AcMNPV infection of AcMNPV-nonpermissive SpLi-221 cells. Our results demonstrated that Splt-p49 could not inhibit the apoptosis induced by AcMNPV infection of SpLi-221 cells when it was under the control of its native promoter. Western blot analysis showed that Splt-P49 was poorly expressed. When the expression of Splt-P49 was under the control of Drosophila hsp70 promoter, the expression of Splt-P49 was advanced, and a higher level of Splt-P49 was detected. As a result, the apoptosis of SpLi-221 cells was inhibited; however, budded-virus production did not improve in comparison with that in AcMNPV-infected SpLi-221 cells. These data indicated that there are other barrier(s) to AcMNPV replication in the nonpermissive SpLi-221 cells besides apoptosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. J.F. Kerr, A.H. Wyllie, A.R. Currie, Br. J. Cancer 26, 239–257 (1972)

    CAS  PubMed  Google Scholar 

  2. Y. Shen, T.E. Shenk, Curr. Opin. Genet. Dev. 5, 105–111 (1995)

    Article  CAS  PubMed  Google Scholar 

  3. H. Steller, Science 267, 1445–1449 (1995)

    Article  CAS  PubMed  Google Scholar 

  4. S.J. Riedl, Y. Shi, Nat. Rev. Mol. Cell Biol. 5, 897–907 (2004)

    Article  CAS  PubMed  Google Scholar 

  5. R.J. Clem, Curr. Drug Targets 8, 1069–1074 (2007)

    Article  CAS  PubMed  Google Scholar 

  6. T. Luque, R. Finch, N. Crook, D.R. O’Reilly, D. Winstanley, J. Gen. Virol. 82, 2531–2547 (2001)

    CAS  PubMed  Google Scholar 

  7. C.S. Duckett, V.E. Nava, R.W. Gedrich, R.J. Clem, J.L. Vandongen, M.C. Gilfillan, H. Shiels, J.M. Hardwick, C.B. Thompson, EMBO J 15, 2685–2694 (1996)

    CAS  PubMed  Google Scholar 

  8. B.A. Hay, D.A. Wassarman, G.M. Rubin, Cell 83, 1253–1262 (1995)

    Article  CAS  PubMed  Google Scholar 

  9. P. Liston, N. Roy, K. Tamai, C. Lefebvre, S. Baird, G. Chertonhorvat, R. Farahani, M. Mclean, J.E. Ikeda, A. Mackenzie, R.G. Korneluk, Nature 379, 349–353 (1996)

    Article  CAS  PubMed  Google Scholar 

  10. J.C. Means, T. Penabaz, R.J. Clem, Virology 358, 436–447 (2007)

    Article  CAS  PubMed  Google Scholar 

  11. R.J. Clem, M. Fechheimer, L.K. Miller, Science 254, 1388–1390 (1991)

    Article  CAS  PubMed  Google Scholar 

  12. D. Xue, H.R. Horvitz, Nature 377, 248–251 (1995)

    Article  CAS  PubMed  Google Scholar 

  13. J. Bertin, S.M. Mendrysa, D.J. LaCount, S. Gaur, J.F. Krebs, R.C. Armstrong, K.J. Tomaselli, P.D. Friesen, J. Virol. 70, 6251–6259 (1996)

    CAS  PubMed  Google Scholar 

  14. D.R. Green, J.C. Reed, Science 281, 1309–1312 (1998)

    Article  CAS  PubMed  Google Scholar 

  15. N.J. Bump, M. Hackett, M. Hugunin, Science 269, 1885–1888 (1995)

    Article  CAS  PubMed  Google Scholar 

  16. A.J. Fisher, W. Cruz, S.J. Zoog, C.L. Schneider, P.D. Friesen, EMBO J. 18, 2031–2039 (1999)

    Article  CAS  PubMed  Google Scholar 

  17. G. Xu, M. Cirilli, Y. Huang, R.L. Rich, D.G. Myszka, H. Wu, Nature 410, 494–497 (2001)

    Article  CAS  PubMed  Google Scholar 

  18. Q. Zhou, J.F. Krebs, S.J. Snipas, A. Price, E.S. Alnemri, K.J. Tomaselli, G.S. Salvesen, Biochemistry 37, 10757–10765 (1998)

    Article  CAS  PubMed  Google Scholar 

  19. S.J. Zoog, J.J. Schiller, J.A. Wetter, N. Chejanovsky, P.D. Friesen, EMBO J. 21, 5130–5140 (2002)

    Article  CAS  PubMed  Google Scholar 

  20. M.P. Guy, P.D. Friesen, J. Virol. 82, 7504–7514 (2008)

    Article  CAS  PubMed  Google Scholar 

  21. Q. Du, D. Lehavi, O. Faktor, Y. Qi, N. Chejanovsky, J. Virol. 73, 1278–1285 (1999)

    CAS  PubMed  Google Scholar 

  22. Z. Pei, G. Reske, Q. Huang, B.D. Hammock, Y. Qi, N. Chejanovsky, J. Biol. Chem. 277, 48677–48684 (2002)

    Article  CAS  PubMed  Google Scholar 

  23. E.A. Herniou, J.A. Olszewski, J.S. Cory, D.R. O’Reilly, Ann. Rev. Entomol. 48, 211–234 (2003)

    Article  CAS  Google Scholar 

  24. S.R. Palli, G.F. Caputo, S.S. Sohi, A.J. Brownwright, T.R. Ladd, B.J. Cook, M. Primavera, B.M. Arif, A. Retnakaran, Virology 222, 201–213 (1996)

    Article  CAS  PubMed  Google Scholar 

  25. N. Chejanovsky, E. Gershburg, Virology 209, 519–525 (1995)

    Article  CAS  PubMed  Google Scholar 

  26. P. Zhang, K. Yang, X. Dai, Y. Pang, D. Su, J. Gen. Virol. 83, 3003–3011 (2002)

    CAS  PubMed  Google Scholar 

  27. Y. Pang, J. Yu, L. Wang, Virology 287, 391–404 (2001)

    Article  CAS  PubMed  Google Scholar 

  28. Q. Yu, T. Lin, G. Feng, K. Yang, Y. Pang, J. Gen. Virol. 89, 1873–1880 (2008)

    Article  CAS  PubMed  Google Scholar 

  29. M. Yu, Z. Li, K. Yang, T. Lin, Y. Gong, L. Pan, Y. Pang, Virus Genes 31, 145–151 (2005)

    Article  CAS  PubMed  Google Scholar 

  30. P. Zhang, K. Yang, X. Dai, Y. Pang, D. Su, Prog. Biochem. Biophys. 29(5), 702–707 (2002)

    CAS  Google Scholar 

  31. E. Gershburg, H. Rivkin, N. Chejanovsky, J. Virol. 71, 7593–7599 (1997)

    CAS  PubMed  Google Scholar 

  32. V.A. Luckow, S.C. Lee, G.F. Barry, P.O. Olins, J. Virol. 67, 4566–4579 (1993)

    CAS  PubMed  Google Scholar 

  33. G. Li, Y. Pang, Q. Chen, Z. Su, X. Wen, Chin. J. Biol. Control 18, 132–134 (2002)

    CAS  Google Scholar 

  34. D.R. O’Reilly, L.K. Miller, V.A. Luckow, Baculovirus Expression Vectors: A Laboratory Manual (W.H. Freeman and Co, New York, 1992)

    Google Scholar 

  35. T. Yanase, C. Yasunaga, T. Kawarabata, Acta Virol. 42, 293–298 (1998)

    CAS  PubMed  Google Scholar 

  36. J.L. Vaughn, R.H. Goodwin, G.J. Tompkins, P. McCawley, In Vitro 13, 213–217 (1977)

    Article  CAS  PubMed  Google Scholar 

  37. R.R. Granados, G.X. Li, A.C. Derksen, K.A. McKenna, J. Invertebr. Pathol. 64, 260–266 (1994)

    Article  Google Scholar 

  38. D.K. Bideshi, B.A. Federici, J. Gen. Virol. 81, 1593–1599 (2000)

    CAS  PubMed  Google Scholar 

  39. G. Lin, G.W. Blissard, J. Virol. 76, 2770–2779 (2002)

    Article  CAS  PubMed  Google Scholar 

  40. M. Lalioti, J. Heath, Nucleic Acids Res. 29, E14 (2001)

    Article  CAS  PubMed  Google Scholar 

  41. W. Wu, T. Lin, L. Pan, M. Yu, Z. Li, Y. Pang, K. Yang, J. Virol. 80, 11475–11485 (2006)

    Article  CAS  PubMed  Google Scholar 

  42. D. Zuidema, A. Schouten, M. Usmany, A.J. Maule, G.J. Belsham, J. Roosien, E.C. Klinge-Roode, J.W. van Lent, J.M. Vlak, J. Gen. Virol. 71, 2201–2209 (1990)

    Article  CAS  PubMed  Google Scholar 

  43. J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual, 3rd edn. (Cold Spring Harbor Laboratory Press, New York, 2001)

    Google Scholar 

  44. R.J. Clem, L.K. Miller, Mol. Cell. Biol. 14, 5212–5222 (1994)

    CAS  PubMed  Google Scholar 

  45. L.K. Miller, A. Lu, in Baculoviruses, ed. by L.K. Miller (Plenum Press, New York, 1997), pp. 193–216

  46. T.D. Morris, L.K. Miller, Virology 197, 339–348 (1993)

    Article  CAS  PubMed  Google Scholar 

  47. S.M. Thiem, X. Du, M.E. Quentin, M.M. Berner, J. Virol. 70, 2221–2229 (1996)

    CAS  PubMed  Google Scholar 

  48. S.M. Thiem, N. Chejanovsky, Virology 319, 292–305 (2004)

    Article  CAS  PubMed  Google Scholar 

  49. B. Bryant, R.J. Clem, J. Gen. Virol. 90, 654–661 (2009)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Major Basic Research Program of China (973 Program) (no. 2009CB118903), the Hi-tech Research and Development Program of China (863 Program) (no. 2006AA10A210), the National Natural Science Foundation of China (no. 30530540 and 30900941).

Author information

Authors and Affiliations

  1. State Key Laboratory of Biocontrol, Sun Yat-sen University, 510275, Guangzhou, China

    Tiehao Lin, Mei Yu, Wenbi Wu, Qian Yu, Qingbei Weng, Kai Yang, Meijin Yuan & Yi Pang

Authors
  1. Tiehao Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mei Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wenbi Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qian Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Qingbei Weng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kai Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Meijin Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yi Pang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Meijin Yuan.

Additional information

Tiehao Lin and Mei Yu contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, T., Yu, M., Wu, W. et al. Functional analysis of Spodoptera litura nucleopolyhedrovirus p49 gene during Autographa californica nucleopolyhedrovirus infection of SpLi-221 cells. Virus Genes 41, 441–449 (2010). https://doi.org/10.1007/s11262-010-0520-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-010-0520-5

Keywords

Search

Navigation