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Identification and functional analysis of the putative anti-apoptotic gene iap4 of Spodoptera litura nucleopolyhedrovirus

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Abstract

Baculoviruses possess two types of anti-apoptotic genes, p35 and inhibitor of apoptosis (iap). In this study, we cloned a novel IAP family member, Splt-IAP4, from Spodoptera litura nucleopolyhedrovirus (SpltNPV). ScanProsite analysis of Splt-IAP4 amino acid sequence show that it contain a baculovirus IAP repeat (BIR) motif at the N-terminal portion from 18 to 61 amino acid and a Zinc finger RING motif near its C-terminus. Baculovirus IAPs could be divided into five types from IAP1 to IAP5 according to amino acid sequence homology, so the Splt-IAP4 was grouped into IAP4 because of containing a single BIR motif. Alignment of the six IAP4s in sequenced baculovirus genomes indicated that the Splt-IAP4 gene shared 74% amino acid sequence similarity with Spodoptera littoralis NPV (SpliNPV) IAP4 higher than other baculovirus IAP4 proteins, suggesting evolutionary conservation. In transient expression assay, Splt-IAP4 was not able to suppress apoptosis induced by a mutant AcMNPV deficient in p35 (vAcAnh) infection and rescue the mutant virus replication from apoptosis in Sf-9 cells. Cell viabilities analysis showed that Splt-IAP4 could not inhibit or delay the apoptosis of Sf-9 cells induced by vAcAnh infection. In summary, Splt-IAP4 had no anti-apoptotic function during vAcAnh-infected Sf-9 cells.

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Acknowledgments

This research was supported by the National Nature Science Foundation of China (No. 30530540), the National Basic Research Program of China (973 Program) (No. 2003CB114202), and the Hi-tech Research and Development Program of China (863 Program) (No. 2006AA10A210).

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Authors and Affiliations

  1. College of Light Industry and Food Science, Zhongkai University of Agiculture and Engineering, Guangzhou, 510225, People’s Republic of China

    Qian Yu

  2. State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Qian Yu

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Correspondence to Qian Yu.

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Yu, Q. Identification and functional analysis of the putative anti-apoptotic gene iap4 of Spodoptera litura nucleopolyhedrovirus. Virus Genes 41, 298–304 (2010). https://doi.org/10.1007/s11262-010-0513-4

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