Abstract
Recent studies have shown that dual mutations in fp25K and p35 of Autographa californica nucleopolyhedrovirus (AcMNPV) result in a typical apoptotic infection on Trichoplusia ni cells, suggesting the involvement of FP25K on NPV-induced apoptosis. To examine the effect of fp25K deletion on Bombyx mori NPV (BmNPV)-induced apoptosis, we generated a BmNPV mutant, fp-p35D, in which both fp25K and p35 genes are deleted from the genome, and compared its phenotype with wild-type (T3), fp25K-deleted (fp-null), and p35-deleted (p35D) BmNPVs. In BmN cells, p35D, but not T3 or fp-null, caused apoptosis with caspase-3 activation. Infection with fp-p35D also resulted in caspase-3 activation, but the level was comparable to that of p35D. Also, we did not observe any apoptotic responses in hemocytes from larvae infected with p35D or fp-p35D. These results indicate that unlike AcMNPV, deletion of fp25K does not affect the pathway of p35D-induced apoptosis of BmN cells and B. mori larvae.
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Acknowledgments
We are grateful to the Molecular Entomology Laboratory, RIKEN (Wako, Japan) for the gift of the p35 deletion plasmid. This work was supported by grants from MEXT (No. 17018007 to T. S., No. 19688004 to S. K., Professional Program for Agricultural Bioinformatics) and MAFF-NIAS (Agrigenome Research Program), Japan.
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Nakanishi, T., Shimada, T. & Katsuma, S. Characterization of a Bombyx mori nucleopolyhedrovirus mutant lacking both fp25K and p35 . Virus Genes 41, 144–148 (2010). https://doi.org/10.1007/s11262-010-0492-5
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DOI: https://doi.org/10.1007/s11262-010-0492-5