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Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China

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Abstract

In this study, four strains of Japanese Encephalitis Virus (JEV) were isolated from the cerebrospinal fluid of aborted fetuses or stillborn piglets collected randomly from a number of piggeries in central China. The E genes were cloned by RT–PCR and sequenced. Phylogenetic analysis was performed with 48 JEV isolates previously reported in China and other countries, and showed that all four isolates can be classified into the subcluster of genotype III. The results strongly suggest that the genotype III of JEV is the major variant currently circulating in the swinery of central China.

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Acknowledgments

The authors gratefully acknowledge the critical review by Dr. Norman A. Gregson (UCL, London, UK). This work was supported by the Science and Technology Projects for Public Welfare of the Ministry of Agriculture (200803015) and Public Interest Project of Henan Province (2008-145) and China Postdoctoral Science Foundation (20090450929).

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Authors and Affiliations

  1. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, People’s Republic of China

    Jian-Ming Fan, Jun Luo, Man Teng, Dan Bu, Fang-Yu Wang, Li Wang & Gai-Ping Zhang

  2. The Laboratory of Toxicology, College of Public Health, Zhengzhou University, 450001, Zhengzhou, People’s Republic of China

    Jian-Ming Fan

  3. College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, People’s Republic of China

    Lu Chen & Chuan-Qing Wang

Authors
  1. Jian-Ming Fan
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  2. Jun Luo
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  3. Lu Chen
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  4. Man Teng
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  5. Dan Bu
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  6. Fang-Yu Wang
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  7. Li Wang
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  8. Chuan-Qing Wang
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  9. Gai-Ping Zhang
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Corresponding author

Correspondence to Gai-Ping Zhang.

Additional information

Jian-Ming Fan and Jun Luo contributed equally to this work.

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Fan, JM., Luo, J., Chen, L. et al. Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China. Virus Genes 40, 357–361 (2010). https://doi.org/10.1007/s11262-010-0464-9

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  • DOI: https://doi.org/10.1007/s11262-010-0464-9

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