Molecular characterization of a human group C rotavirus detected first in Turkey
- 180 Downloads
The present study was done to find out the prevalence of group B and C rotavirus infections in children with diarrhea presented at two major hospitals in Ankara, Turkey. Group B rotavirus was not found in any samples. One of 122 samples was positive for group C rotavirus. Phylogenetic analysis of genes for nonstructural protein NSP4, and structural proteins VP4, VP6, and VP7 confirmed the human origin of this strain. Similar to other human group C rotaviruses, one N-glycosylation site was predicted at amino acid residue 67 on the VP7 of strain GUP188. The genes of strain GUP188 were closely related to those of human group C rotavirus strain from the UK (Bristol) for NSP4, China (208 and Wu82) for VP4 and VP6, and from Colombia (Javeriana) for VP7, indicating that the Turkish group C rotavirus was unique and can serve as an additional reference strain for the molecular epidemiology of group C rotaviruses.
KeywordsViral diarrhea Group C rotavirus Turkey Molecular epidemiology
This research was supported by a Grant-in-Aid Scientific Research B from Japan Society for the Promotion of Sciences; grant number: 17406013.
- 18.A. Castello, M. Argüelles, G. Villegas, N. López, D. Ghiringhelli, L. Semorile, G. Glikmann, J. Med. Virol. 62, 199–207 (2000). doi: 10.1002/1096-9071(200010)62:2<199::AID-JMV11>3.0.CO;2-X CrossRefPubMedGoogle Scholar
- 42.A. Steele, V. James, J. Clin. Microbiol. 37, 4142–4144 (1997)Google Scholar