Skip to main content

Tensaw virus genome sequence and its relation to other Bunyaviridae

Virus Genes volume 39, pages 309–318 (2009)Cite this article

Abstract

Tensaw virus (TSV) belongs to the genus Orthobunyavirus within the Bunyaviridae family. Although TSV does not cause hemorrhagic fever as some other members of its family, serological studies have shown that serum from Florida residents react against TSV indicating viral infection in humans. In this study, the three RNA genome segments of a TSV isolated from Anopheles crucians mosquitoes collected in North Central Florida in 2006 and a TSV isolate obtained from the CDC, Fort Collins, were sequenced and compared to other Bunyaviridae. The placement of the TSVs within the Bunyamwera serogroup was confirmed by phylogenetic analysis of the inferred amino acid (aa) sequence of proteins coded by each of the RNA segments separately as well as by the combined tree of the same three inferred proteins. The N terminal glycoprotein (Gn) encoded by the M segment contained the 18 conserved Cysteines present in Bunyamwera and California serogroups, the two glycosylation sites, and residues considered potential proteolytic cleavage sites conserved in other Bunyaviridae. The TSV L protein displayed all the strictly conserved amino acids in the four conserved regions known to be catalytically active for the RNA dependent RNA polymerase transcriptase and replicase activities. The amino acid conservation between the two TSV viral isolates was 100, 99.4, and 99.6% for the S, M, and L segments, respectively.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3

References

  1. R.M. Elliott, M. Bouloy, C.H. Calisher, R. Goldbach, J.T. Moyer, S.T. Nichol, R. Pettersson, A. Plyusnin, C.S. Schmaljohn, in Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, ed. by M.H.V. van Regenmortel, C.M. Fauget, D.H.L. Bishop (Academic Press, San Diego, California, 2000), p. 599

    Google Scholar 

  2. S.S. Soldan, F. González-Scarano, J. Neurovirol. 11, 412–423 (2005)

    Article  Google Scholar 

  3. C.H. Calisher, N. Karabatsos, in The Arboviruses: Epidemiology, Ecology, ed. by T.P. Monath (CRC Press, Boca Raton, FL, 1988), p. 19

    Google Scholar 

  4. J. Casals, L. Whitman, Am. J. Trop. Med. Hyg. 9, 73–77 (1960)

    CAS  Article  Google Scholar 

  5. R.E. Shope, O.R. Causey, Am. J. Trop. Med. Hyg. 11, 283–290 (1962)

    CAS  Article  Google Scholar 

  6. W.D. Sudia, P.H. Coleman, R.W. Chamberlain, Am. J. Trop. Med. Hyg. 18, 98–102 (1969)

    CAS  Article  Google Scholar 

  7. R.W. Chamberlain, W.D. Sudia, P.H. Coleman, Am. J. Trop. Med. Hyg. 18, 92–97 (1969)

    CAS  Article  Google Scholar 

  8. J.N. Nagayama, N. Komar, J.F. Levine, C.S. Apperson, Vector Borne Zoonotic Dis. 1, 169–171 (2001)

    CAS  Article  Google Scholar 

  9. D.I. Ortiz, A. Wozniak, M.W. Tolson, P.E. Turner, Vector Borne Zoonotic Dis. 5, 20–32 (2005)

    CAS  Article  Google Scholar 

  10. C.H. Calisher, J.S. Lazuick, S. Lieb, T.P. Monath, K.G. Castro, Am. J. Trop. Med. Hyg 39, 117–122 (1988)

    CAS  Article  Google Scholar 

  11. D.H.L. Bishop, in The Bunyaviridae, ed. by R.M. Elliott (Plenum, New York, 1996), p. 19

    Chapter  Google Scholar 

  12. R.M. Elliott, J. Gen. Virol. 70, 1281–1285 (1989)

    CAS  Article  Google Scholar 

  13. B.J. Beaty, M. Holterman, W. Tabachnick, R.E. Shope, E.J. Rozhon, D.H.L. Bishop, Science 211, 1433–1435 (1981)

    CAS  Article  Google Scholar 

  14. R.E. Shope, E.J. Rozhon, D.H.L. Bishop, Virology 114, 273–276 (1981)

    CAS  Article  Google Scholar 

  15. N. Lopez, R. Muller, C. Prehaud, M. Bouloy, J. Virol. 69, 3972–3979 (1995)

    CAS  PubMed  PubMed Central  Google Scholar 

  16. H. Jin, R.M. Elliott, J. Virol. 67, 1396–1404 (1993)

    CAS  PubMed  PubMed Central  Google Scholar 

  17. R.M. Elliott, Virology 173, 426–436 (1989)

    CAS  Article  Google Scholar 

  18. J.F. Lees, C.R. Pringle, R.M. Elliott, Virology 148, 1–14 (1986)

    CAS  Article  Google Scholar 

  19. H. Akashi, Y. Kaku, X.G. Kong, H. Pang, J. Gen. Virol. 78, 2847–2851 (1997)

    CAS  Article  Google Scholar 

  20. Y. Ogawa, K. Kato, Y. Tohya, H. Akashi, Arch. Virol. 152, 1679–1686 (2007)

    CAS  Article  Google Scholar 

  21. T. Yanase, K. Yoshida, S. Ohashi, T. Kato, T. Tsuda, Virus Res. 93, 63–69 (2003)

    CAS  Article  Google Scholar 

  22. R.S. Bennett, D.R. Ton, C.T. Hanson, B.R. Murphy, S.S. Whitehead, Virol. J. 4, 41 (2007). doi:https://doi.org/10.1186/1743-422X-4-41

    Article  Google Scholar 

  23. V.H. Aquino, L.T. Figueiredo, J. Virol. Methods 115, 51–57 (2004)

    CAS  Article  Google Scholar 

  24. V.H. Aquino, M.L. Moreli, L.T. Moraes Figueiredo, Arch. Virol. 148, 19–28 (2003)

    CAS  Article  Google Scholar 

  25. E.F. Dunn, D.C. Pritlove, R.M. Elliott, J. Gen. Virol. 75, 597–608 (1994)

    CAS  Article  Google Scholar 

  26. R.M. Elliott, in The Bunyaviridae, ed. by R.M. Elliott (Plenum, New York, 1996), p. 295

    Chapter  Google Scholar 

  27. A.J. Lambert, R.S. Lanciotti, J. Gen. Virol. 89, 2580–2585 (2008)

    CAS  Article  Google Scholar 

  28. G. Kuno, C.J. Mitchell, G.-J.J. Chang, G.C. Smith, J. Clin, Microbiol 34, 1184–1188 (1996)

    CAS  Google Scholar 

  29. C.L. Brockus, P.R. Grimstad, Virus Genes 19, 73–83 (1999)

    CAS  Article  Google Scholar 

  30. W.P. Campbell, C. Huang, Virus Res. 61, 137–144 (1999)

    CAS  Article  Google Scholar 

  31. T. Briese, A. Rambaut, W.I. Lipkin, J. Gen. Virol. 85, 3071–3077 (2004)

    CAS  Article  Google Scholar 

  32. R.M. Elliott, A. McGregor, Virology 171, 516–524 (1989)

    CAS  Article  Google Scholar 

  33. R. Müller, O. Poch, M. Delarue, D.H.L. Bishop, M. Bouloy, J. Gen. Virol. 75, 1345–1352 (1994)

    Article  Google Scholar 

  34. M. Delarue, O. Poch, N. Tordo, D. Moras, P. Argos, Protein Eng. 3, 461–467 (1990)

    CAS  Article  Google Scholar 

  35. L.F. Cassidy, J.L. Petterson, Antimicrob. Agents Chemother. 33, 2009–2011 (1989)

    CAS  Article  Google Scholar 

  36. C. Sibold, H. Meisel, D.H. Kruger, M. Labuda, J. Lysy, O. Kozuch, M. Pejcoch, A. Vaheri, A. Plyusnin, J. Virol. 73, 667–675 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  37. S.R. Gerrard, L. Li, A.D. Barrett, S.T. Nichol, J. Virol. 78, 8922–8926 (2004)

    CAS  Article  Google Scholar 

  38. P.H. Coleman, Am. J. Trop. Med. Hyg. 18, 81–91 (1969)

    CAS  Article  Google Scholar 

  39. C.H. Calisher, in The Bunyaviridae, ed. by R.M. Elliott (Plenum, New York, 1996), p. 1

    Google Scholar 

  40. A. Roberts, C. Rossier, D. Kolakofsky, N. Nathanson, F. Gonzalez-Scarano, Virology 206, 742–745 (1995)

    CAS  Article  Google Scholar 

  41. B.R. Quinan, C.L. de Brito Magalhães, R.F.V. Novaes, J.R. dos Santos, E.G. Kroon, C.A. Bonjardim, P.C.P. Ferreira, Virus Genes 36, 435–437 (2008)

    CAS  Article  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. Barbara Johnson for providing the TSV FE3-66FB isolate, Dr. Verena Lietze, Dan Fitzpatrick, and Leah May for editing the manuscript and Chris Maggio for help with a figure. This research was funded by a USDA-CSREES, T-STAR Grant # 00057913.

Author information

Affiliations

  1. Entomology & Nematology Department, University of Florida, P.O. Box 110620, Gainesville, FL, 32611-0620, USA

    Stacey L. Watts, Alejandra Garcia-Maruniak & James E. Maruniak

Authors
  1. Stacey L. Watts
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alejandra Garcia-Maruniak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James E. Maruniak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to James E. Maruniak.

Additional information

The nucleotide sequences data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers FJ943505 to FJ943510.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Watts, S.L., Garcia-Maruniak, A. & Maruniak, J.E. Tensaw virus genome sequence and its relation to other Bunyaviridae . Virus Genes 39, 309–318 (2009). https://doi.org/10.1007/s11262-009-0400-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-009-0400-z

Keywords

  • Tensaw virus
  • Orthobunyavirus
  • Virus genome sequence
  • Bunyaviridae phylogeny
  • Bunyamwera serogroup