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Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein

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Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF 51 (Bm51) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm51 was characterized. Transcripts of Bm51 were detected from 4.5 through 72 hour post infection (h p.i.) by RT-PCR. The corresponding protein was detected from 6 to 72 h p.i. in BmNPV-infected BmN cells by western blot analysis using a polyclonal antibody against Bm51. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm51 encodes a 23-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. The protein was temporarily called BV-E23. In addition immunofluorescence microscopy demonstrated that the protein was present within the cytoplasm and nuclei in virus-infected cells. In conclusion, the available data suggest that Bm51 is a functional ORF of BmNPV and encodes a protein expressed in the early stage of the infection cycle that is associated with the BV envelope.

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References

  1. C.X. Zhang, X.C. Ma, Z.J. Guo, Virology 333, 190–199 (2005). doi:https://doi.org/10.1016/j.virol.2004.12.028

    Article  CAS  PubMed  Google Scholar 

  2. B.A. Keddie, G.W. Aponte, L.E. Volkman, Science 243, 1728–1730 (1989). doi:https://doi.org/10.1126/science.2648574

    Article  CAS  PubMed  Google Scholar 

  3. L.K. Miller, J. Invertebr. Pathol. 65, 211–216 (1995). doi:https://doi.org/10.1006/jipa.1995.1032

    Article  CAS  PubMed  Google Scholar 

  4. C.L. Merrington, M.J. Bailey, R.D. Possee, Mol. Biotechnol. 8, 283–297 (1997). doi:https://doi.org/10.1007/BF02760782

    Article  CAS  PubMed  Google Scholar 

  5. R.M. Patterson, J.K. Selkirk, B.A. Merrick, Environ. Health Perspect 103, 756–759 (1995). doi:https://doi.org/10.2307/3432869

    CAS  PubMed  PubMed Central  Google Scholar 

  6. A. Huser, C. Hofmann, Am. J. Pharmacogenomics 3, 53–63 (2003). doi:https://doi.org/10.2165/00129785-200303010-00007

    Article  CAS  PubMed  Google Scholar 

  7. H. Tani, C.K. Limn, C.C. Yap et al., J. Virol. 77, 9799–9808 (2003). doi:https://doi.org/10.1128/JVI.77.18.9799-9808.2003

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. S. Gomi, K. Majima, S. Maeda, J. Gen. Virol. 80(Pt 5), 1323–1337 (1999)

    Article  CAS  PubMed  Google Scholar 

  9. H.J. Xu, Z.N. Yang, F. Wang, C.X. Zhang, Arch. Virol. 151, 681–695 (2006). doi:https://doi.org/10.1007/s00705-005-0664-0

    Article  CAS  PubMed  Google Scholar 

  10. H.Q. Chen, K.P. Chen, Q. Yao, Z.J. Guo, L.L. Wang, FEBS Lett. 581, 5836–5842 (2007). doi:https://doi.org/10.1016/j.febslet.2007.11.059

    Article  CAS  PubMed  Google Scholar 

  11. J.Q. Ge, Z.N. Yang, X.D. Tang, H.J. Xu, J. Hong, J.G. Chen et al., J. Gen. Virol. 89, 766–774 (2008). doi:https://doi.org/10.1099/vir.0.83398-0

    Article  CAS  PubMed  Google Scholar 

  12. J.Q. Ge, J.F. Zhao, Y.M. Shao, C.H. Tian, C.X. Zhang, Mol. Biol. Rep. (2008). doi:https://doi.org/10.1007/s11033-008-9212-9

    Article  PubMed  Google Scholar 

  13. M.F. Du, X.M. Yin, Z.J. Guo, L.J. Zhu, J. Biochem. Mol. Biol. 39, 737–742 (2006)

    CAS  PubMed  Google Scholar 

  14. K. Okano, V.S. Mikhailov, S. Maeda, J. Virol. 73, 110–119 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  15. M. Iwanaga, M. Kurihara, M. Kobayashi, W. Kang, Virology 297, 39–47 (2002). doi:https://doi.org/10.1006/viro.2002.1443

    Article  CAS  PubMed  Google Scholar 

  16. D.R. O’Reilly, L.K. Miller, V.A. Luckow, Baculovirus Expression Vectors (W.H. Freeman and Company, New York, 1992)

    Google Scholar 

  17. R.D. Appel, A. Bairoch, D.F. Hochstrasser, Trends Biochem. Sci. 19, 258–260 (1994). doi:https://doi.org/10.1016/0968-0004(94)90153-8

    Article  CAS  PubMed  Google Scholar 

  18. S.F. Altschul, T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller et al., Nucleic Acids Res. 25, 3389–3402 (1997). doi:https://doi.org/10.1093/nar/25.17.3389

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. W.R. Pearson, Methods Enzymol. 3, 63–98 (1990). doi:https://doi.org/10.1016/0076-6879(90)83007-V

    Article  Google Scholar 

  20. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994). doi:https://doi.org/10.1093/nar/22.22.4673

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. M.M. Bradford, Anal. Biochem. 72, 248–254 (1976). doi:https://doi.org/10.1016/0003-2697(76)90527-3

    Article  CAS  PubMed  Google Scholar 

  22. M. Iwanaga, W.K. Kang, M. Kobayashi, S. Maeda, Arch. Virol. 145, 1763–1773 (2000). doi:https://doi.org/10.1007/s007050070054

    Article  CAS  PubMed  Google Scholar 

  23. S.C. Braunagel, M.D. Summers, Virology 202, 315–328 (1994). doi:https://doi.org/10.1006/viro.1994.1348

    Article  CAS  PubMed  Google Scholar 

  24. P. Caballero, D. Zuidema, C. Santiago Alvarez, J.M. Vlak, Biocontrol. Sci. Technol. 2, 145–157 (1992)

    Article  Google Scholar 

  25. W.F.J. Ijkel, D. Westenberg, M.R.W. Goldbach, G.W. Blissard, J.M. Vlak, D. Zuidema, Virology 275, 30–41 (2000). doi:https://doi.org/10.1006/viro.2000.0483

    Article  CAS  PubMed  Google Scholar 

  26. A. Lu, E.B. Carstens, Virology 190, 201–209 (1992)

    Article  CAS  PubMed  Google Scholar 

  27. L.E. Volkman, P.A. Goldsmith, Virology 143(1), 185–195 (1985). doi:https://doi.org/10.1016/0042-6822(85)90107-2

    Article  CAS  PubMed  Google Scholar 

  28. H.J. Xu, Z.N. Yang, J.F. Zhao, C.H. Tian, J.Q. Ge, X.D. Tang et al., J. Gen. Virol. 89, 1212–1219 (2008). doi:https://doi.org/10.1099/vir.0.83633-0

    Article  CAS  PubMed  Google Scholar 

  29. W.C. Rice, L.K. Miller, Virus. Res. 6, 155–172 (1986). doi:https://doi.org/10.1016/0168-1702(86)90047-X

    Article  CAS  PubMed  Google Scholar 

  30. D.Z. Rohel, P. Faulkner, J. Virol. 50, 739–747 (1984)

    CAS  PubMed  PubMed Central  Google Scholar 

  31. A. Acharya, P.K. Gopinathan, J. Gen. Virol. 83, 2015–2023 (2002)

    Article  CAS  PubMed  Google Scholar 

  32. J.J. Liu, E.B. Carstens, Virology 223, 396–400 (1996). doi:https://doi.org/10.1006/viro.1996.0494

    Article  CAS  PubMed  Google Scholar 

  33. A.M. Crawford, L.K. Miller, J. Virol. 62, 2773–2781 (1988)

    CAS  PubMed  PubMed Central  Google Scholar 

  34. K.L. Hefferon, A.G. Oomens, S.A. Monsma, C.M. Finnerty, G.W. Blissard, Virology 258, 455–468 (1999). doi:https://doi.org/10.1006/viro.1999.9758

    Article  CAS  PubMed  Google Scholar 

  35. S.A. Monsma, A.G.P. Oomens, G.W. Blissard, J. Virol. 70, 4607–4616 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  36. M.M. Rahman, K.P. Gopinathan, Virus. Res. 94, 45–57 (2003). doi:https://doi.org/10.1016/S0168-1702(03)00123-0

    Article  CAS  PubMed  Google Scholar 

  37. S.C. Braunagel, S.T. Williamson, S. Saksena, Z. Zhong, W.K. Russell, D.H. Russell et al., Proc. Natl. Acad. Sci. USA 101, 8372–8377 (2004). doi:https://doi.org/10.1073/pnas.0402727101

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  38. J.M. Slack, S.D. Lawrence, J. Gen. Virol. 86, 1637–1643 (2005). doi:https://doi.org/10.1099/vir.0.80832-0

    Article  CAS  PubMed  Google Scholar 

  39. S.C. Braunagel, J.K. Burks, G. Rosas-Acosta, R.L. Harrison, H. Ma, M.D. Summers, J. Virol. 73, 8559–8570 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  40. D. Wang, S.H. An, Z.J. Guo, H.J. Xu, C.X. Zhang, Arch. Virol. 150, 1505–1515 (2005). doi:https://doi.org/10.1007/s00705-005-0534-9

    Article  CAS  PubMed  Google Scholar 

  41. H.J. Xu, Y.H. Liu, Z.N. Yang, C.X. Zhang, JBMB 39, 263–269 (2006)

    CAS  PubMed  Google Scholar 

  42. M.N. Pearson, C. Groten, G.F. Rohrmann, J. Virol. 74, 6126–6131 (2000). doi:https://doi.org/10.1128/JVI.74.13.6126-6131.2000

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  43. O.Y. Lung, M. Cruz-Alvarez, G.W. Blissard, J. Virol. 77, 328–339 (2003). doi:https://doi.org/10.1128/JVI.77.1.328-339.2003

    Article  CAS  Google Scholar 

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Acknowledgments

This project was supported by the National Program of High-tech Research and Development (863 High-Tech Program, No. 2006AA10A119) and the National Natural Science Foundation of China (Project No. 30570074).

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Authors and Affiliations

  1. Institute of Insect Science, Zhejiang University, Kaixuan Road 268, Hangzhou, 310029, People’s Republic of China

    C.-H. Tian, X.-D. Tang, H.-J. Xu, J.-Q. Ge, Y.-G. Miao & C.-X. Zhang

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  1. C.-H. Tian
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Correspondence to C.-X. Zhang.

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Tian, CH., Tang, XD., Xu, HJ. et al. Bombyx mori nucleopolyhedrovirus ORF51 encodes a budded virus envelope associated protein. Virus Genes 38, 171–177 (2009). https://doi.org/10.1007/s11262-008-0312-3

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  • DOI: https://doi.org/10.1007/s11262-008-0312-3

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