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Virus Genes

, Volume 38, Issue 1, pp 19–29 | Cite as

Genome-wide reduction in transcriptomal profiles of varicella-zoster virus vaccine strains compared with Parental Oka strain using long oligonucleotide microarrays

  • Esther Grinfeld
  • Alan Ross
  • Thorsten Forster
  • Peter Ghazal
  • Peter G. E. KennedyEmail author
Article

Abstract

Varicella-Zoster virus (VZV) causes varicella as a primary infection following which it becomes latent in human ganglia and then reactivates to cause herpes zoster. VZV vaccines are used to prevent primary infection with varicella, and also to reduce the incidence of viral reactivation causing herpes zoster and post-herpetic neuralgia. To gain further insights into the molecular basis of their attenuated virulence, we used long oligonucleotide microarrays to determine the lytic transcriptomal profiles of two vaccine VZV strains (Merck and GSK) compared with the Oka parental (P-Oka) strain. There was a global downregulation of transcription of both vaccines relative to P-Oka, although this downregulation was more extensive in the GSK strain. Open Reading Frames (ORFs) 62 and 14 were the most transcriptionally downregulated on the arrays for both vaccines compared with the parental strain.

Keywords

Varicella-zoster virus Microarray Genome Open reading frame 

Notes

Acknowledgements

This work was supported by a project grant to PGEK from the Chief Scientist Office (CSO) of Scotland. PGEK has acted on one occasion as a consultant to Sanofi Pasteur MSD.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Esther Grinfeld
    • 1
  • Alan Ross
    • 2
  • Thorsten Forster
    • 2
  • Peter Ghazal
    • 2
  • Peter G. E. Kennedy
    • 1
    Email author
  1. 1.Department of Neurology, Division of Clinical Neurosciences, Institute of Neurological SciencesSouthern General HospitalGlasgowScotland, UK
  2. 2.Division of Pathway MedicineUniversity of Edinburgh Medical SchoolEdinburghScotland, UK

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