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First step in characterization of cis-acting sequences involved in fowl adenovirus 1 (CELO) packaging and its effect on the development of a helper-dependent vector strategy

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Abstract

Adenovirus-based vectors are widely developed for potential utilization as vectors in vaccine and gene therapy strategies. We focused on developing a helper-dependent adenoviral (HD-Ad) vector for the potential use of CELO, a member of the Aviadenovirus genus, in avian species vaccination. Our aim was to localize sequences which could play an essential role in CELO genome encapsidation and, when deleted, was unable to produce viruses to develop a helper CELO virus. A panel of 6 mutants with deletions between nt 80 and 350 of the CELO genome was constructed and characterized for its ability to produce viable virus. To develop a helper-dependent adenoviral vector derived from CELO, a helper virus was developed by inserting loxP sequences around the region containing the identified putative packaging sequences. A LMH (Leghorn Male Hepatocarcinoma) cell line expressing Cre recombinase was developed to allow the excision of this region. We demonstrated that the region from nt 200 to 250 was important and the region from nt 250 to 300 at the left end of the CELO genome was essential for virus encapsidation. We also showed that the loxP-flanked region was efficiently removed in a Cre expressing cell line to produce a candidate helper virus.

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References

  1. C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball, Virus taxonomy. Classification and Nomenclature of Viruses. Eight Report of the International Committee on the Taxonomy of Viruses (Elsevier Academic Press, 2005)

  2. D.S. Bangari, S.K. Mittal, Vaccine 24, 849–862 (2006). doi:https://doi.org/10.1016/j.vaccine.2005.08.101

    Article  CAS  PubMed  Google Scholar 

  3. N. Tatsis, H.C. Ertl, Mol. Ther. 10, 616–629 (2004). doi:https://doi.org/10.1016/j.ymthe.2004.07.013

    Article  CAS  PubMed  Google Scholar 

  4. W.C. Russell, J. Gen. Virol. 81, 2573–2604 (2000)

    Article  CAS  PubMed  Google Scholar 

  5. R. Alba, A. Bosch, M. Chillon. Gene Ther. 12(Suppl 1), S18–S27 (2005). doi:https://doi.org/10.1038/sj.gt.3302612

    Article  CAS  PubMed  Google Scholar 

  6. M.A. Morsy, M. Gu, S. Motzel, J. Zhao, J. Lin, Q. Su et al., Proc. Natl. Acad. Sci. USA 95, 7866–7871 (1998). doi:https://doi.org/10.1073/pnas.95.14.7866

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. M. Havenga, R. Vogels, D. Zuijdgeest, K. Radosevic, S. Mueller, M. Sieuwerts et al., J. Gen. Virol. 87, 2135–2143 (2006). doi:https://doi.org/10.1099/vir.0.81956-0

    Article  CAS  PubMed  Google Scholar 

  8. P.S. Reddy, S. Ganesh, M.P. Limbach, T. Brann, A. Pinkstaff, M. Kaloss et al., Virology 311, 384–393 (2003). doi:https://doi.org/10.1016/S0042-6822(03)00161-2

    Article  Google Scholar 

  9. A.A. Lemckert, J. Grimbergen, S. Smits, E. Hartkoorn, L. Holterman, B. Berkhout et al., J. Gen. Virol. 87, 2891–2899 (2006). doi:https://doi.org/10.1099/vir.0.82079-0

    Article  CAS  PubMed  Google Scholar 

  10. L. Holterman, R. Vogels, R. van der Vlugt, M. Sieuwerts, J. Grimbergen, J. Kaspers et al., J. Virol. 78, 13207–13215 (2004). doi:https://doi.org/10.1128/JVI.78.23.13207-13215.2004

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. E.J. Kremer, S. Boutin, M. Chillon, O. Danos, J. Virol. 74, 505–512 (2000)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. A. Keriel, C. Rene, C. Galer, J. Zabner, E.J. Kremer, J. Virol. 80, 1487–1496 (2006). doi:https://doi.org/10.1128/JVI.80.3.1487-1496.2006

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. S. Roy, Y. Zhi, G.P. Kobinger, J. Figueredo, R. Calcedo, J.R. Miller et al., J. Gen. Virol. 87, 2477–2485 (2006). doi:https://doi.org/10.1099/vir.0.81989-0

    Article  CAS  PubMed  Google Scholar 

  14. P.S. Reddy, N. Idamakanti, Y. Chen, T. Whale, L.A. Babiuk, M. Mehtali et al., J. Virol. 73, 9137–9144 (1999)

    CAS  PubMed  PubMed Central  Google Scholar 

  15. P.S. Reddy, N. Idamakanti, L.A. Babiuk, M. Mehtali, S.K. Tikoo, J. Gen. Virol. 80(Pt 11), 2909–2916 (1999)

    Article  CAS  PubMed  Google Scholar 

  16. J.S. Rothel, D.B. Boyle, G.W. Both, A.D. Pye, J.G. Waterkeyn, P.R. Wood et al., Parasite Immunol. 19:221–227. (1997). doi:https://doi.org/10.1046/j.1365-3024.1997.d01-200.x

    Article  CAS  PubMed  Google Scholar 

  17. A. Francois, C. Chevalier, B. Delmas, N. Eterradossi, D. Toquin, G. Rivallan et al., Vaccine 22, 2351–2360 (2004). doi:https://doi.org/10.1016/j.vaccine.2003.10.039

    Article  CAS  PubMed  Google Scholar 

  18. S.F. Farina, G.P. Gao, Z.Q. Xiang, J.J. Rux, R.M. Burnett, M.R. Alvira et al., J. Virol. 75, 11603–11613 (2001). doi:https://doi.org/10.1128/JVI.75.23.11603-11613.2001

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. S. Chiocca, R. Kurzbauer, G. Schaffner, A. Baker, V. Mautner, M. Cotten, J. Virol. 70, 2939–2949 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  20. S. Washietl, F. Eisenhaber, BMC Bioinformatics 4, 55 (2003). doi:https://doi.org/10.1186/1471-2105-4-55

    Article  PubMed Central  PubMed  Google Scholar 

  21. V. Payet, C. Arnauld, J.P. Picault, A. Jestin, P. Langlois, J. Virol. 72, 9278–9285 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  22. A. Francois, N. Eterradossi, B. Delmas, V. Payet, P. Langlois, J. Virol. 75, 5288–5301 (2001). doi:https://doi.org/10.1128/JVI.75.11.5288-5301.2001

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. R.J. Parks, L. Chen, M. Anton, U. Sankar, M.A. Rudnicki, F.L. Graham, Proc. Natl. Acad. Sci. USA 93, 13565–13570 (1996). doi:https://doi.org/10.1073/pnas.93.24.13565

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. M. Grable, P. Hearing, J. Virol. 64, 2047–2056 (1990)

    CAS  PubMed  PubMed Central  Google Scholar 

  25. M. Grable, P. Hearing, J. Virol. 66, 723–731 (1992)

    CAS  PubMed  PubMed Central  Google Scholar 

  26. S.I. Schmid, P. Hearing, J. Virol. 71, 3375–3384 (1997)

    CAS  PubMed  PubMed Central  Google Scholar 

  27. P. Hearing, R.J. Samulski, W.L. Wishart, T. Shenk, J. Virol. 61, 2555–2558 (1987)

    CAS  PubMed  PubMed Central  Google Scholar 

  28. C. Soudais, S. Boutin, S.S. Hong, M. Chillon, O. Danos, J.M. Bergelson et al., J. Virol. 74, 10639–10649 (2000). doi:https://doi.org/10.1128/JVI.74.22.10639-10649.2000

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  29. L. Xing, S.K. Tikoo, Virology 314, 650–661 (2003). doi:https://doi.org/10.1016/S0042-6822(03)00493-8

    Article  CAS  PubMed  Google Scholar 

  30. C. Soudais, S. Boutin, E.J. Kremer, Mol. Ther. 3, 631–640 (2001). doi:https://doi.org/10.1006/mthe.2001.0263

    Article  CAS  PubMed  Google Scholar 

  31. L. Xing, S.K. Tikoo, Virus Res. 104, 207–214 (2004). doi:https://doi.org/10.1016/j.virusres.2004.04.007

    Article  CAS  PubMed  Google Scholar 

  32. L. Xing, S.K. Tikoo, Virus Res. (2007)

  33. L. Xing, L. Zhang, J. Van Kessel, S.K. Tikoo, J. Gen. Virol. 84, 2947–2956 (2003). doi:https://doi.org/10.1099/vir.0.19418-0

    Article  CAS  PubMed  Google Scholar 

  34. W. Zhang, M.J. Imperiale, J. Virol. 74, 2687–2693 (2000). doi:https://doi.org/10.1128/JVI.74.6.2687-2693.2000

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  35. W. Zhang, J.A. Low, J.B. Christensen, M.J. Imperiale, J. Virol. 75, 10446–10454 (2001). doi:https://doi.org/10.1128/JVI.75.21.10446-10454.2001

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  36. K.E. Gustin, M.J. Imperiale, J. Virol. 72, 7860–7870 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  37. K.E. Gustin, P. Lutz, M.J. Imperiale, J. Virol. 70, 6463–6467 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  38. P. Ostapchuk, J. Yang, E. Auffarth, P. Hearing, J. Virol. 79, 2831–2838 (2005). doi:https://doi.org/10.1128/JVI.79.5.2831-2838.2005

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  39. P. Perez-Romero, K.E. Gustin, M.J. Imperiale, J. Virol. 80, 1965–1971 (2006). doi:https://doi.org/10.1128/JVI.80.4.1965-1971.2006

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  40. P. Ostapchuk, M.E. Anderson, S. Chandrasekhar, P. Hearing, J. Virol. 80, 6973–6981 (2006). doi:https://doi.org/10.1128/JVI.00123-06

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  41. S.G. Ewing, S.A. Byrd, J.B. Christensen, R.E. Tyler, M.J. Imperiale, J. Virol. (2007)

  42. F. Le Goff, I. Mederle-Mangeot, A. Jestin, P. Langlois, J. Gen. Virol. 86, 2019–2027 (2005). doi:https://doi.org/10.1099/vir.0.80879-0

    Article  CAS  PubMed  Google Scholar 

  43. J.M. Thomson, W.A. Parrott. Biotechniques 24, 922–924, 926, 928 (1998)

    Article  CAS  PubMed  Google Scholar 

  44. S.I. Schmid, P. Hearing, J. Virol. 72, 6339–6347 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  45. Y. Maeda, E. Kimura, Y. Uchida, Y. Nishida, S. Yamashita, T. Arima et al., Virology 309, 330–338 (2003). doi:https://doi.org/10.1016/S0042-6822(02)00137-X

    Article  CAS  PubMed  Google Scholar 

  46. P. Ng, R.J. Parks, F.L. Graham, Methods Mol. Med. 69, 371–388 (2002)

    CAS  PubMed  Google Scholar 

  47. P.S. Reddy, K. Sakhuja, S. Ganesh, L. Yang, D. Kayda, T. Brann et al., Mol. Ther. 5, 63–73 (2002). doi:https://doi.org/10.1006/mthe.2001.0510

    Article  CAS  PubMed  Google Scholar 

  48. M. Candolfi, J.F. Curtin, W.D. Xiong, K.M. Kroeger, C. Liu, A. Rentsendorj, H. Agadjanian, L. Medina-Kauwe, D. Palmer, P. Ng, P.R. Lowenstein, M.G. Castro, Mol. Ther. (2006)

  49. P. Ng, C. Beauchamp, C. Evelegh, R. Parks, F.L. Graham, Mol. Ther. 3, 809–815 (2001). doi:https://doi.org/10.1006/mthe.2001.0323

    Article  CAS  PubMed  Google Scholar 

  50. P. Umana, C.A. Gerdes, D. Stone, J.R. Davis, D. Ward, M.G. Castro et al., Nat. Biotechnol. 19, 582–585 (2001). doi:https://doi.org/10.1038/89349

    Article  CAS  PubMed  Google Scholar 

  51. R. Alba, P. Hearing, A. Bosch, M. Chillon, Virology (2007)

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Acknowledgements

We would like to thank A. Jestin for the critical reading and correcting of the manuscript. We also thank our colleagues C. Bernard, and B. Grasland for the critical reading of the manuscript and N. Franck, D. Dory and Y. Blanchard for their helpful advice and discussions.

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  1. Unité de génétique virale et biosécurité, Agence Française de Sécurité Sanitaire des Aliments, Site des Croix, BP 53 22440, Ploufragan, France

    Claire Barra & Patrick Langlois

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  1. Claire Barra
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  2. Patrick Langlois
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Correspondence to Patrick Langlois.

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Barra, C., Langlois, P. First step in characterization of cis-acting sequences involved in fowl adenovirus 1 (CELO) packaging and its effect on the development of a helper-dependent vector strategy. Virus Genes 38, 46–55 (2009). https://doi.org/10.1007/s11262-008-0281-6

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  • DOI: https://doi.org/10.1007/s11262-008-0281-6

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