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Sequence analysis of the gene coding for the S1 glycoprotein of infectious bronchitis virus (IBV) strains from New Zealand

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Abstract

Four new infectious bronchitis virus (IBV) strains (T6, K32, K43, and K87) were isolated from clinically infected chickens in New Zealand. These strains were compared with four strains (A, B, C, and D), which had circulated 25 years previously, by sequencing the gene coding for the S1 subunit of the spike glycoprotein. Analysis of the nucleotide and deduced amino acid sequences revealed that the eight strains from New Zealand are genetically related and share greater than 82.8% nucleotide and 79% amino acid homology within the S1 region. Strains T6, K43, and K87 were more than 99% homologous to previously described strains C and D. A fourth new strain (K32) was most closely related to the previously described B strain. Phylogenetic analysis of strains revealed that New Zealand strains were more closely related to Australian than European or North American strains. The New Zealand A strain shared 99.5% nucleotide and 98.7% amino acid homology with the Australian Vic S strain. Deduced amino acid sequence of the S1 glycoprotein indicated differences between strains that were, in general, consistent with virus neutralization patterns.

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Acknowledgments

The work was supported in part by funds provided by PacificVet Ltd., New Zealand, and we are thankful to Kent Deitemeyer and Drs. Neil Christensen and Jon Hickford for advice and practical assistance. We thank Drs. Lance Jennings and Barry Palmer for their constructive suggestions.

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Authors and Affiliations

  1. Cell Biology Group, Agriculture and Life Sciences Division, Lincoln University, Christchurch, 7647, New Zealand

    Robin McFarlane

  2. Department of Animal Biotechnology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India

    Ramneek Verma

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  1. Robin McFarlane
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  2. Ramneek Verma
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Correspondence to Ramneek Verma.

Additional information

The nucleotide sequence data reported in this article have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers A = AF151953, B = AF151954, C = AF151955, D = AF151956, K32 = AF151957, K43 = AF151958, K87 = AF151959, and T6 = AF151960.

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McFarlane, R., Verma, R. Sequence analysis of the gene coding for the S1 glycoprotein of infectious bronchitis virus (IBV) strains from New Zealand. Virus Genes 37, 351–357 (2008). https://doi.org/10.1007/s11262-008-0273-6

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