Skip to main content
SpringerLink
Log in

Characterization of Citrus tristeza virus strains from southern China based on analysis of restriction patterns and sequences of their coat protein genes

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Citrus tristeza virus (CTV) isolates collected from southern China were characterized by biological indexing on citrus indicators, restriction fragment length polymorphism (RFLP), and bi-directional reverse transcription-polymerase chain reaction (BD-PCR) analysis of their coat protein (CP) genes. Of the 30 isolates, only two isolates, N3 and N4, did not induce visible symptoms. Twenty-eight other isolates induced stem pitting and vein clearing, plant stunting, and leaf yellowing symptoms on Mexican lime, Duncan grapefruit, and sour orange seedlings. In BD-PCR analysis, a 392-bp fragment specific for the mild strains was amplified from isolates N3 and N4, and a 320-bp fragment specific for the severe strains was produced from the other 28 isolates. The RFLP analysis for RT-PCR products of the CP gene with restriction enzyme HinfI identified seven groups representing groups I–VI and a new group, which was not involved in the seven groups defined by Gillings (J Virol Methods 44:305–317, 1993). The sequences of the CP genes from 12 Chinese CTV isolates showed a high divergence, with 91.5–99.7% identities at the nucleotide level and 94.2–99.6% identities at the amino acid level. Our results suggest that the composition of CTV populations from China has a high genetic diversity in the CP gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. V.J. Febres, L. Ashoulin, M. Mawassi, A. Frank, M. Bar-Joseph, K.L. Manjunath et al., Phytopathology 86, 1331–1335 (1996)

    CAS  Google Scholar 

  2. J.F. Ballester-Olmos, J.A. Pina, E. Carbonell, P. Moreno, A. Hermoso de Mendoza, M. Cambra et al., Plant Pathol. 42, 219–229 (1993). doi:https://doi.org/10.1111/j.1365-3059.1993.tb01494.x

    Article  Google Scholar 

  3. C.N. Roistacher, P. Moreno, in Proceedings of the 11th Conference of the International Organization of Citrus Virologists, ed. by R.H. Brlansky, R.F. Lee, L.W. Timmer (IOCV, Riverside, CA, 1991), pp. 7–19

  4. M. Bar-Joseph, R. Marcus, R.F. Lee, Annu. Rev. Phytopathol. 27, 291–316 (1989). doi:https://doi.org/10.1146/annurev.py.27.090189.001451

    Article  Google Scholar 

  5. R.F. Lee, M. Bar-Joseph, in Compendium of Citrus Diseases, 2nd edn., ed. by L.W. Timmer, S.M. Garnsey, J.H. Graham (2000), pp. 61–63

  6. C.L. Niblett, H. Genc, B. Cevik, S. Halbert, L. Brown, G. Nolasco et al., Virus Res. 71, 97–106 (2000). doi:https://doi.org/10.1016/S0168-1702(00)00191-X

    Article  CAS  Google Scholar 

  7. M.A. Ayllón, L. Rubio, V. Sentandreu, A. Moya, J. Guerri, P. Moreno, Virus Genes 32, 119–128 (2006). doi:https://doi.org/10.1007/s11262-005-6866-4

    Article  Google Scholar 

  8. V. Sentandreu, J.A. Castro, M.A. Ayllón, L. Rubio, J. Guerri, F. González-Candelas et al., Arch. Virol. 151, 875–894 (2006). doi:https://doi.org/10.1007/s00705-005-0683-x

    Article  CAS  Google Scholar 

  9. M.R. Albiach-Martí, J. Guerri, A. Hermoso de Mendoza, F. Laigret, J.F. Ballester-Olmos, P. Moreno, Phytopathology 90, 134–138 (2000). doi:https://doi.org/10.1094/PHYTO.2000.90.2.134

    Article  Google Scholar 

  10. M.R. Albiach-Martí, M. Mawassi, S. Gowda, T. Satyanarayana, M.E. Hilf, S. Shanker et al., J. Virol. 74, 6856–6865 (2000). doi:https://doi.org/10.1128/JVI.74.15.6856-6865.2000

    Article  Google Scholar 

  11. A.V. Karasev, V.P. Boyko, S. Gowda, O.V. Nikolaeva, M.E. Hilf, E.V. Koonin et al., Virology 208, 511–520 (1995). doi:https://doi.org/10.1006/viro.1995.1182

    Article  CAS  Google Scholar 

  12. M. Mawassi, E. Mietkiewska, R. Gofman, G. Yang, M. Bar-Joseph, J. Gen. Virol. 77, 2359–2364 (1996). doi:https://doi.org/10.1099/0022-1317-77-9-2359

    Article  CAS  Google Scholar 

  13. M.C. Vives, L. Rubio, C. López, J. Navas-Castillo, M.R. Albiach-Martí, W.O. Dawson et al., J. Gen. Virol. 80, 811–816 (1999)

    Article  CAS  Google Scholar 

  14. Z.N. Yang, D.H. Mathews, J.A. Dodds, T.E. Mirkov, Virus Genes 19, 131–142 (1999). doi:https://doi.org/10.1023/A:1008127224147

    Article  CAS  Google Scholar 

  15. G. Suastika, T. Natsuaki, H. Terui, T. Kano, H. Ieki, S. Okuda, J. Gen. Plant Pathol. 67, 73–77 (2001). doi:https://doi.org/10.1007/PL00012992

    Article  CAS  Google Scholar 

  16. S. Ruiz-Ruiz, P. Moreno, J. Guerri, S. Ambrós, Arch. Virol. 151, 387–398 (2006). doi:https://doi.org/10.1007/s00705-005-0618-6

    Article  CAS  Google Scholar 

  17. M.A. Rocha-Peña, R.F. Lee, R. Lastra, C.L. Niblett, F.M. Ochoa-Corona, S.M. Garnsey, R.K. Yokomi, Plant Dis. 79, 437–445 (1995)

    Article  Google Scholar 

  18. C. Vela, M. Cambra, E. Cortés, C. Pérez San Román, A. Sainz, P. Moreno et al., J. Gen. Virol. 67, 91–96 (1986). doi:https://doi.org/10.1099/0022-1317-67-1-91

    Article  Google Scholar 

  19. T.A. Permar, S.M. Garnsey, D.J. Gumpf, R.F. Lee, Phytopathology 80, 224–228 (1990). doi:https://doi.org/10.1094/Phyto-80-224

    Article  Google Scholar 

  20. H.R. Pappu, S.S. Pappu, K.L. Manjunath, R.F. Leet, C.L. Niblett, Proc. Natl. Acad. Sci. USA 90, 3641–3644 (1993). doi:https://doi.org/10.1073/pnas.90.8.3641

    Article  CAS  Google Scholar 

  21. B. Cevik, S.S. Pappu, H.R. Pappu, D. Benscher, M. Irey, R.F. Lee et al., in Proceedings of the 13th Conference of the International Organization of Citrus Virologists, ed. by J.V. da Graça, P. Moreno, R.K. Yokomi (IOCV, Riverside, CA,1996), pp. 17–24

  22. Z. Huang, P.A. Rundell, X. Guan, C.A. Powell, Plant Dis. 88, 625–629 (2004). doi:https://doi.org/10.1094/PDIS.2004.88.6.625

    Article  CAS  Google Scholar 

  23. M. Gillings, P. Broadbent, J. Indsto, R. Lee, J. Virol. Methods 44, 305–317 (1993). doi:https://doi.org/10.1016/0166-0934(93)90065-Y

    Article  CAS  Google Scholar 

  24. L. Rubio, M.A. Ayllón, J. Guerri, H. Pappu, C. Niblett, P. Moreno, Ann. Appl. Biol. 129, 479–489 (1996). doi:https://doi.org/10.1111/j.1744-7348.1996.tb05770.x

    Article  CAS  Google Scholar 

  25. M.R. Albiach-Martí, J. Guerri, M. Cambra, S.M. Garnsey, P. Moreno, J. Virol. Methods 88, 25–34 (2000). doi:https://doi.org/10.1016/S0166-0934(00)00165-8

    Article  Google Scholar 

  26. J.K. Zhang, N. Hong, G.P. Wang, J. Agric. Biotechnol. 14, 259–264 (2006)

    CAS  Google Scholar 

  27. C.X. Wang, N. Hong, G.P. Wang, J.K. Zhang, H. Liu, J. Plant Pathol. 89, 377–383 (2007)

    CAS  Google Scholar 

  28. S.M. Garnsey, E.M. Civerolo, D.J. Gumpf, R.K. Yokomi, R.F. Lee, in Proceedings of the 11th Conference on IOCV (Riverside, 1991), pp. 113–120

  29. C.X. Wang, G.P. Wang, N. Hong, B. Jiang, H. Liu, K.W. Wu, Chin. J. Biotechnol. 22, 629–634 (2006)

    CAS  Google Scholar 

  30. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994). doi:https://doi.org/10.1093/nar/22.22.4673

    Article  CAS  Google Scholar 

  31. S. Kumar, K. Tamura, I.B. Jakobsen, M. Nei, Bioinformatics 17, 1244–1245 (2001). doi:https://doi.org/10.1093/bioinformatics/17.12.1244

    Article  CAS  Google Scholar 

  32. L. Rubio, M.A. Ayllón, P. Kong, A. Fernández, M.L. Polek, J. Guerri et al., J. Virol. 75, 8054–8062 (2001). doi:https://doi.org/10.1128/JVI.75.17.8054-8062.2001

    Article  CAS  Google Scholar 

  33. M.A. Ayllón, C. López, J. Navas-Castillo, S.M. Garnsey, J. Guerri, R. Flores et al., Arch. Virol. 146, 27–40 (2001). doi:https://doi.org/10.1007/s007050170188

    Article  Google Scholar 

  34. P. Kong, L. Rubio, M. Polek, B.W. Falk, Virus Genes 21, 139–145 (2000). doi:https://doi.org/10.1023/A:1008198311398

    Article  CAS  Google Scholar 

  35. C. López, M.A. Ayllón, J. Navas-Castillo, J. Guerri, P. Moreno, R. Flores, Phytopathology 88, 685–691 (1998). doi:https://doi.org/10.1094/PHYTO.1998.88.7.685

    Article  Google Scholar 

  36. N.G. Iglesias, S.P. Gago-Zachert, G. Robledo, N. Costa, M.I. Plata, O. Vera, O. Grau, L.C. Semorile, Virus Genes 36, 199–207 (2008). doi:https://doi.org/10.1007/s11262-007-0169-x

    Article  CAS  Google Scholar 

  37. A. Roy, P. Ramachandran, R.H. Brlansky, Arch. Virol. 148, 707–722 (2003)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the Ministry of Education of China (Grant No: IRT0548) and Ministry of Agriculture of China (Grant No: nyhyzx 07-023). The authors would like to thank Prof. G. P. Martelli, University of Bari, Italy, for providing the antiserum and the protocol for virus purification. We also thank Prof. G. Q. Li, Huazhong Agricultural University, and X. D. Li, Shandong Agricultural University, for critical reading of the manuscript.

Author information

Authors and Affiliations

  1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Bo Jiang, Ni Hong, Guo-Ping Wang, Jian-Kun Zhang, Cai-Xia Wang, Yong Liu & Xu-Dong Fan

  2. National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Bo Jiang, Guo-Ping Wang & Yong Liu

  3. Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, 96822, USA

    John Hu

Authors
  1. Bo Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ni Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guo-Ping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. John Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jian-Kun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cai-Xia Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xu-Dong Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ni Hong, Guo-Ping Wang or John Hu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, B., Hong, N., Wang, GP. et al. Characterization of Citrus tristeza virus strains from southern China based on analysis of restriction patterns and sequences of their coat protein genes. Virus Genes 37, 185–192 (2008). https://doi.org/10.1007/s11262-008-0254-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-008-0254-9

Keywords

Search

Navigation