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Complete nucleotide sequence and experimental host range of Okra mosaic virus

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Abstract

Okra mosaic virus (OkMV) is a tymovirus infecting members of the family Malvaceae. Early infections in okra (Abelmoschus esculentus) lead to yield losses of 12–19.5%. Besides intensive biological characterizations of OkMV only minor molecular data were available. Therefore, we determined the complete nucleotide sequence of a Nigerian isolate of OkMV. The complete genomic RNA (gRNA) comprises 6,223 nt and its genome organization showed three major ORFs coding for a putative movement protein (MP) of Mr 73.1 kDa, a large replication-associated protein (RP) of Mr 202.4 kDa and a coat protein (CP) of Mr 19.6 kDa. Prediction of secondary RNA structures showed three hairpin structures with internal loops in the 5′-untranslated region (UTR) and a 3′-terminal tRNA-like structure (TLS) which comprises the anticodon for valine, typical for a member of the genus Tymovirus. Phylogenetic comparisons based on the RP, MP and CP amino acid sequences showed the close relationship of OkMV not only to other completely sequenced tymoviruses like Kennedya yellow mosaic virus (KYMV), Turnip yellow mosaic virus (TYMV) and Erysimum latent virus (ErLV), but also to Calopogonium yellow vein virus (CalYVV), Clitoria yellow vein virus (CYVV) and Desmodium yellow mottle virus (DYMoV). This is the first report of a complete OkMV genome sequence from one of the various OkMV isolates originating from West Africa described so far. Additionally, the experimental host range of OkMV including several Nicotiana species was determined.

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Acknowledgements

We thank Dirk Bellstedt, Kees Pleij and Theo Dreher for scientific discussion and Agnes Pietruszka and Marianne Koerbler for technical assistance.

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Authors and Affiliations

  1. Institute of Plant Diseases and Plant Protection, Leibniz University Hannover, Herrenhaeuser Str. 2, 30419, Hannover, Germany

    Dirk Stephan & Edgar Maiss

  2. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada, N1G 2W1

    Mahbuba Siddiqua

  3. Plant Protection Research Institute, Vietnam Academy of Agricultural Science, Dongngac, Tuliem, Hanoi, Vietnam

    Anh Ta Hoang

  4. Microbiology and Biosafety, BBA Institute for Plant Virology, Messeweg 11/12, 38104, Braunschweig, Germany

    Jill Engelmann

  5. Plant Virus Department, DSMZ, Messeweg 11/12, 38104, Braunschweig, Germany

    Stephan Winter

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  1. Dirk Stephan
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Correspondence to Dirk Stephan.

Additional information

The nucleotide sequence data reported in this article have been submitted to the Genbank nucleotide sequence database and have been assigned the accession number EF554577.

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Stephan, D., Siddiqua, M., Ta Hoang, A. et al. Complete nucleotide sequence and experimental host range of Okra mosaic virus . Virus Genes 36, 231–240 (2008). https://doi.org/10.1007/s11262-007-0181-1

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