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Herpes simplex virus protein UL11 but not UL51 is associated with lipid rafts

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Abstract

The UL11 and UL51 gene products of herpes simplex virus (HSV) are membrane-associated tegument proteins that are incorporated into the HSV virion. UL11 and UL51 are conserved throughout the herpesvirus family. Both UL11 and UL51, either singly or in combination, are involved in virion envelopment and/or egress. Both proteins are fatty acylated: UL11 is both acylated by myristoic and palmitoic acids and UL51 is monoacylated by palmitoic acid. Using confocal microscopy and sucrose gradient fractionations in transfected or HSV-infected cells, we found that HSV-2 UL11 but not UL51 was associated with lipid rafts. The dual acylation of UL11 was necessary for lipid raft association, as mutations in the myristoylation or palmitoylation sites prevented lipid raft association. These differences in lipid raft association may contribute to the functional differences between UL11 and UL51.

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Acknowledgments

Caco-2 cells were kindly provided by Dr. Yukako. Fujinaga (Osaka Univ. Japan). We would like to thank E. Iwata, T. Tsuruguchi and H. Noma for technical support.

This research was supported in part by Grants-in-Aid for Scientific Research and Grants-in-Aid for Scientific Research in Priority Areas from the Ministry of Education Culture, Sports, Science and Technology (MEXT) of Japan and the Japan Society for the Promotion of Science (JSPS). T.K. was supported by a JSPS Research Fellowship for Young Scientists.

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Authors and Affiliations

  1. Department of Virology, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, 466-8550, Japan

    Tetsuo Koshizuka, Yasushi Kawaguchi, Naoki Nozawa, Isamu Mori & Yukihiro Nishiyama

  2. Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, 567-0085, Japan

    Tetsuo Koshizuka

  3. Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, 108-8639, Japan

    Yasushi Kawaguchi

  4. PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012, Japan

    Yasushi Kawaguchi

  5. Department of Virology I, National Institute of Infectious Disease, Shinjuku-ku, Tokyo, 162-8640, Japan

    Naoki Nozawa

  6. Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Aichi, 480-1195, Japan

    Isamu Mori

Authors
  1. Tetsuo Koshizuka
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  2. Yasushi Kawaguchi
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  5. Yukihiro Nishiyama
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Correspondence to Yukihiro Nishiyama.

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Koshizuka, T., Kawaguchi, Y., Nozawa, N. et al. Herpes simplex virus protein UL11 but not UL51 is associated with lipid rafts. Virus Genes 35, 571–575 (2007). https://doi.org/10.1007/s11262-007-0156-2

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  • DOI: https://doi.org/10.1007/s11262-007-0156-2

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