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Molecular characterization of two Chinese isolates of Beet mosaic virus

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Abstract

The complete genomic sequences of Beet mosaic virus Xinjiang (BtMV-XJ) and Inner Mongolia (BtMV-IM) isolates from China were determined and compared with US and German isolates, reported previously. Results showed that viral genome of the two isolates both comprise 9,591 nucleotides, and contain the large single open reading frame (ORF) encoding a single polyprotein of 3,085 amino acid residues, from which ten putative functional proteins may be produced by autolytic cleavage processing as the US (BtMV-Wa) and German (BtMV-G) isolates. Sequence comparisons showed that BtMV-XJ shared 89.8% and 98.3% overall nucleotide identity with BtMV-Wa and BtMV-G isolates, and BtMV-IM exhibited the overall identities of 91.6% and 93.8% with BtMV-Wa and BtMV-G, respectively. Further, analyses revealed that BtMV-XJ shared higher identities in almost every region to BtMV-G than to BtMV-Wa both at the nucleotide and the amino acid levels. While BtMV-IM in the regions (6,666–7,671 and 7,672–9,591) showed highest homology with BtMV-XJ and BtMV-G, especially, after nt 7,672 with similarity up to 99.2% with BtMV-G; the region (2,331–4,083) showed highest identity (98.0% nt identity) with BtMV-Wa. That suggested BtMV-XJ had a more close relationship to BtMV-G, while BtMV-IM was more likely to be a natural recombination virus. In addition, phylogenetic analysis of the available BtMV CP sequences showed that BtMV isolates fell into two distinct groups: Euroasia group (Europe and China) and America group (USA). To the best of our knowledge, this study reported the complete sequences of two BtMV isolates from Asia for the first time.

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Acknowledgments

The authors would like to thank Professor Zhang Huizhong and Hao Xiuying for their helping on collection of sugar beet samples. This work was partially supported by the National Natural Science Foundation of China (30471136 and 30671359).

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Authors and Affiliations

  1. Department of Plant Pathology, China Agricultural University, Beijing, 100094, P.R. China

    Haiying Xiang, Yan-Hong Han, Chenggui Han, Dawei Li & Jialin Yu

  2. State Key Laboratory for Agro-Biotechnology, China Agricultural University, Beijing, 100094, P.R. China

    Haiying Xiang, Yan-Hong Han, Chenggui Han, Dawei Li & Jialin Yu

Authors
  1. Haiying Xiang
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  2. Yan-Hong Han
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  3. Chenggui Han
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  4. Dawei Li
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  5. Jialin Yu
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Corresponding author

Correspondence to Chenggui Han.

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H. Xiang and Y.-H. Han contributed equally to this paper.

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Xiang, H., Han, YH., Han, C. et al. Molecular characterization of two Chinese isolates of Beet mosaic virus . Virus Genes 35, 795–799 (2007). https://doi.org/10.1007/s11262-007-0132-x

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