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Molecular cloning and characterization of the DNA adenine methyltransferase gene in Feldmannia sp. virus

Virus Genes volume 34, pages 177–183 (2007)Cite this article

Abstract

The genome of Feldmannia sp. virus (FsV), a marine brown alga virus, contains a putative DNA adenine methyltransferase (dam) gene of 1,245 bp that encodes a polypeptide of 45.8 kDa. A BLAST search with the FsV dam gene showed high amino acid identity to two putative methyltransferase genes, ORF B29 of Feldmannia irregularis virus (FirrV, 54%) and ORF129 of Ectocarpus siliculosus virus (EsV, 36%); and a PSI BLAST search revealed similarity to the N6-adenine methyltransferases (MTases) of other species. Most conserved motifs of β-class MTases were observed in the FsV dam gene. However, neither of the highly conserved sequences in motifs I (FxGxG) or IV [(S/N/D)PP(Y/F/W)] perfectly matched those in the FsV dam gene. The highly conserved DPPY consensus sequence in motif IV was NTPW in the FsV dam gene, perfectly matching the sequences in ORF B29 of FirrV and ORF129 of EsV. Therefore, the dam genes in brown algae viruses may belong to a yet undiscovered group. The FsV Dam protein expressed from the cloned FsV dam gene methylated E. coli chromosomal DNA. This is the first report showing that a virus infecting marine filamentous brown algae encodes a functional Dam protein.

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Acknowledgements

This research was supported by a grant (PF0330601-00) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government. The authors appreciate Prof. Stanley Hattman, Department of Biology, University of Rochester for his careful reading of the manuscript and valuable comments.

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  1. Department of Microbiology, Pukyong National University, 599-1, Daeyeon 3-Dong, Busan, South Korea, 608-737

    Yunjung Park, Gun-Do Kim & Tae-Jin Choi

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  1. Yunjung Park
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  2. Gun-Do Kim
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  3. Tae-Jin Choi
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Correspondence to Tae-Jin Choi.

Additional information

The nucleotide sequence is deposited in GenBank under accession number DQ415647.

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Park, Y., Kim, GD. & Choi, TJ. Molecular cloning and characterization of the DNA adenine methyltransferase gene in Feldmannia sp. virus. Virus Genes 34, 177–183 (2007). https://doi.org/10.1007/s11262-006-0059-7

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  • DOI: https://doi.org/10.1007/s11262-006-0059-7

Keywords

  • Algal virus
  • Feldmannia
  • FsV
  • DNA methylation