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Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates

Virus Genes volume 35, pages 109–117 (2007)Cite this article

An Erratum to this article was published on 01 October 2007

Abstract

The virus most often reported in papaya (Carica papaya L.) is papaya ringspot (PRSV). The aim of this work was the molecular genomic characterization of a Mexican severe isolate of PRSV-P “Mex-VrPO” (isolate from the State of Veracruz in Paso de Ovejas) as well as its comparison with other isolates from other world regions. The linear, assembled, single-strand positive sense RNA genome of PRSV-P Mex-VrPO was 10320 nt in length (excluding the poly(A) tail) and contained a single large predicted ORF with 3344 aa. The comparative analysis of our PRSV isolates and five others reported before, showed the most variable proteins were P1, P3, 6 K and CP with 13–33%, 5–7%, 6–9% and 5–9% divergence respectively. The most conserved ones were CI, NIb and HC-Pro (2–3%, 3–5% and 4–5%). The phylogenetic analysis showed a close relation between the Mexican (Mex-VrPO) and Hawaiian (PRSV-P HA) isolates. This work provided the first opportunity to establish the foundation for (1) understanding whole genome and polyprotein variability between Asian and American PRSV isolates, and (2) elucidating major trends in the relative evolution of viral cistrons as deduced from in silico recombination analyses.

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References

  1. D.E. Purcifull, J. Edwarson, E. Hiebert, Papaya ringspot virus. Descriptions of plant viruses. Commonwealth Mycological Institute and Association of Applied Biologists, United Kingdom (1984)

  2. D.D. Shukla, C.W. Ward, A.A. Brunt, The Potyviridae (CAB International, Wallinford, UK, 1994)

    Google Scholar 

  3. D. Teliz, G. Mora, D. Nieto, D. Gonsalves, E. Garcia, L. Matheis, C. Avila, Revista Mexicana de Fitopatología 9, 64 (1991)

    Google Scholar 

  4. M.F. Bateson, R.E. Lines, P. Revill, W. Chaleeprom, C.V. Ha, A.J. Gibbs, J.L. Dale, J. Gen. Virol. 83, 2575 (2002)

    PubMed  CAS  Google Scholar 

  5. R.K. Jain, J. Sharma, A.S. Sivakumar, P.K. Sharma, A.S. Byadgi, A.K. Verma, A. Varma, Arch. Virol. 149, 2435 (2004)

    PubMed  Article  CAS  Google Scholar 

  6. H.K.C. Laidlaw, D.M. Persley, C.K. Pallaghy, I.D. Godwin, Arch. Virol. 149, 1633 (2004)

    PubMed  Article  CAS  Google Scholar 

  7. L. Silva-Rosales, N. Becerra-Leor, S. Ruiz-Castro, D. Teliz-Ortiz, J.C. Noa-Carrazana, Arch. Virol. 145, 835 (2000)

    PubMed  Article  CAS  Google Scholar 

  8. J.C. Noa-Carrazana, D. González-de-León, B.S. Ruiz-Castro, D. Piñero, L. Silva-Rosales, Plant Dis. 90, 1004 (2006)

    Article  Google Scholar 

  9. S.-D. Yeh, F.J. Jan, C.H. Chiang, T.J. Doong, M.C. Chen, P.H. Chung, H.J. Bau, J. Gen. Virol. 73, 2531 (1992)

    PubMed  CAS  Article  Google Scholar 

  10. C.-H. Wang, (2002)

  11. C.-H. Wang, S.-D. Yeh, R.J. Chiu, D. Gonsalves, Plant Dis. 71, 491 (1987)

    Article  Google Scholar 

  12. P. Attasart, G. Charoensilp, S. Kertbundit, S. Panyim, M. Juricek, Acta Virol. 46, 241 (2002)

    PubMed  CAS  Google Scholar 

  13. M. Worobey, E.C. Holmes, J. Gen. Virol. 80, 2535 (1999)

    PubMed  CAS  Google Scholar 

  14. R.H. Converse, R.R. Martin, ELISA methods for plant viruses. American Phytopathological Society, St. Paul, MN, 1990

  15. J.A. Lindbo, W.G. Dougherty, Virology 189, 725 (1992)

    PubMed  Article  CAS  Google Scholar 

  16. S. Ruiz-Castro, L. Silva-Rosales, Revista Mexicana de Fitopatología 15, 83 (1997)

    Google Scholar 

  17. F. Sanger, S. Nicklen, A.R. Coulson, Proc. Natl. Acad. Sci. USA 74, 5463 (1977)

    PubMed  Article  CAS  Google Scholar 

  18. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673 (1994)

    PubMed  Article  CAS  Google Scholar 

  19. M.O. Salminen, J.K. Carr, D.S. Burke, F.E. McCutchan, AIDS Res. Hum. Retroviruses 11, 1423 (1995)

    PubMed  CAS  Article  Google Scholar 

  20. M.J. Gibbs, J.S. Armstrong, A.J. Gibbs, Bioinformatics 16, 573 (2000)

    PubMed  Article  CAS  Google Scholar 

  21. J. Maynard Smith, J. Mol. Evol. 34, 126 (1992)

    Google Scholar 

  22. D. Posada, K.A. Crandall, Proc. Natl. Acad. Sci. USA 98, 13757 (2001)

    PubMed  Article  CAS  Google Scholar 

  23. M. Padidam, S. Sawyer, C.M. Fauquet, Virology 265, 218 (1999)

    PubMed  Article  CAS  Google Scholar 

  24. D. Martin, E. Rybicki, Bioinformatics 16, 562 (2000)

    PubMed  Article  CAS  Google Scholar 

  25. G. McGuire F. Wright, Bioinformatics 16, 130 (2000)

    Article  Google Scholar 

  26. C.-H. Wang, S.-D. Yeh, Arch. Virol. 142, 271 (1997)

    PubMed  Article  CAS  Google Scholar 

  27. D.R. Gallie, Ann. Rev. Plant Physiol. Plant Mol. Biol. 44, 77 (1993)

    Article  CAS  Google Scholar 

  28. A. Chyi-Ying, A.-B. Shyu, Trends Biochem. Sci. 20, 465 (1995)

    Article  Google Scholar 

  29. M.E. Aleman-Verdaguer, C. Goudou-Urbino, J. Dubern, R.N. Beachy, C. Fauquet, J. Gen. Virol. 78, 1253 (1997)

    PubMed  Google Scholar 

  30. D. Posada, Mol. Biol. Evol. 19, 708 (2002)

    PubMed  CAS  Google Scholar 

  31. C. Desbiez, H. Lecoq, Arch. Virol. 149, 1619 (2004)

    PubMed  Article  CAS  Google Scholar 

  32. R.C. Larsen, P.N. Miklas, K.L. Druffel, Phytopathology 95, 1037 (2003)

    Article  CAS  Google Scholar 

  33. D. Guo, M.-L. Rajama, M. Saarma, J.P. Valkonen, J. Gen. Virol. 82, 935 (2001)

    PubMed  CAS  Google Scholar 

  34. K. Mukherjeel, Y. Verma, S.K. Chakrabarti, S.M.P. Khurana, Virus Genes 29, 229 (2004)

    Article  Google Scholar 

  35. J.C. Carrington, J. Verchot, J. Virol. 69, 3668 (1995)

    PubMed  Google Scholar 

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Acknowledgments

This research was funded by the project SEMARNAT 2002 CO1-304/A-1. JC Noa-Carrazana acknowledges financial support from Cinvestav and CONACyT (scholarship no.111976).

Author information

Affiliations

  1. Cinvestav Campus Guanajuato, Apdo Postal 629, Irapuato, Gto, 36500, Mexico

    Juan Carlos Noa-Carrazana & Laura Silva-Rosales

  2. Paseo del Atardecer 360, Col Villas de Irapuato, Irapuato, Gto, 36670, Mexico

    Diego González-de-León

  3. Laboratorio de Biotecnología y Ecología Aplicada, Universidad Veracruzana. Circuito Los Lagos S/N. Zona Universitaria Campus Sur, Apdo. Postal 250, Xalapa, Veracruz, 91001, Mexico

    Juan Carlos Noa-Carrazana

Authors
  1. Juan Carlos Noa-Carrazana
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  2. Diego González-de-León
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  3. Laura Silva-Rosales
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Correspondence to Laura Silva-Rosales.

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Noa-Carrazana, J.C., González-de-León, D. & Silva-Rosales, L. Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates. Virus Genes 35, 109–117 (2007). https://doi.org/10.1007/s11262-006-0039-y

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  • DOI: https://doi.org/10.1007/s11262-006-0039-y

Keywords

  • Potyvirus
  • Evolution
  • Genome
  • P1
  • Recombination
  • P and W biotypes