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Complete Nucleotide Sequence of the RNA-2 of Grapevine Deformation and Grapevine Anatolian Ringspot Viruses

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Abstract

The nucleotide sequence of RNA-2 of Grapevine Anatolian ringspot virus (GARSV) and Grapevine deformation virus (GDefV), two recently described nepoviruses, has been determined. These RNAs are 3753 nt (GDefV) and 4607 nt (GARSV) in size and contain a single open reading frame encoding a polyprotein of 122 kDa (GDefV) and 150 kDa (GARSV). Full-length nucleotide sequence comparison disclosed 71–73% homology between GDefV RNA-2 and that of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), and 62–64% homology between GARSV RNA-2 and that of Grapevine chrome mosaic virus (GCMV) and Tomato black ring virus (TBRV). As previously observed in other nepoviruses, the 5′ non-coding regions of both RNAs are capable of forming stem-loop structures. Phylogenetic analysis of the three proteins encoded by RNA-2 (i.e. protein 2A, movement protein and coat protein) confirmed that GDefV and GARSV are distinct viruses which can be assigned as definitive species in subgroup A and subgroup B of the genus Nepovirus, respectively.

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Author notes

  1. Nina Abou Ghanem-Sabanadzovic & Sead Sabanadzovic

    Present address: Department of Plant Pathology, University of California, Davis CA, 95616, USA

Authors and Affiliations

  1. Istituto Agronomico Mediterraneo, Valenzano (Bari), Italy

    Nina Abou Ghanem-Sabanadzovic, Sead Sabanadzovic & Michele Digiaro

  2. Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi and Istituto di Virologia Vegetale del CNR, Bari, Italy

    Giovanni P. Martelli

Authors
  1. Nina Abou Ghanem-Sabanadzovic
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  2. Sead Sabanadzovic
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  3. Michele Digiaro
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  4. Giovanni P. Martelli
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Corresponding author

Correspondence to Nina Abou Ghanem-Sabanadzovic.

Additional information

† The nucleotide sequences data reported in this paper were submitted to the GenBank database and given the accession numbers AY291207 for the Grapevine Anatolian ringspot virus and AY291208 for the Grapevine deformation virus.

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Ghanem-Sabanadzovic, N., Sabanadzovic, S., Digiaro, M. et al. Complete Nucleotide Sequence of the RNA-2 of Grapevine Deformation and Grapevine Anatolian Ringspot Viruses. Virus Genes 30, 335–340 (2005). https://doi.org/10.1007/s11262-005-6776-5

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  • DOI: https://doi.org/10.1007/s11262-005-6776-5

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