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Sequence Analysis of the Haemagglutinin and Fusion Protein Genes of Peste-des-petits Ruminants Vaccine Virus of Indian Origin

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Abstract

The amino acid composition of the two surface proteins of peste-des-petits ruminants vaccine virus belonging to lineage four from India were deduced from the nucleotide sequence. The fusion (F) protein gene of PPRV Sungri/96 is 2405 nucleotides long and in relation to the length, it is 80 nucleotides longer than that of PPRV Nigeria/75/1 which are found to be present at the 5′UTR of this virus. The complete F gene alignment with other morbillivirus reveals a homology of 89% with PPRV/Nigeria/75/1 and 48–51% with other morbilliviruses. The F protein of PPRV Sungri/96 exhibited characteristics similarity to those of other morbillivirus F proteins. The overall amino acid similarity with its counterpart PPRV Nigeria/75/1 was 96%; with other morbilliviruses it is 65–74%. The PPRV Sungri/96 haemagglutinin (H) protein gene is 1954 nucleotides long and showed a sequence homology of 90.7% with PPRV/Nigeria/75/1 and with other morbilliviruses it ranged from 33% to 45%. At amino acids level, PPRV Sungri/96 showed a homology of 92.3% with PPRV/Nigeria/75/1 and 34–49% with other morbilliviruses. The phylogenetic tree constructed for F and H gene reveals four separate groups which is very similar to that found in other genes. To the best of our knowledge this is the first report describing the F and H genes of an Indian isolate.

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Acknowledgements

The authors thank Director and Joint Director (Academic), Indian Veterinary Research Institute (IVRI), Izatnagar,Uttar Pradesh, India for providing necessary facilities. D.M thanks the Director, Central Institute of Fisheries Technology (CIFT), Kerala for providing financial assistance for his Doctoral Degree.

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Authors and Affiliations

  1. Indian Veterinary Research Institute, Izatanagar, 243 122, Bareilly, India

    P. Dhar & R. P. Singh

  2. Central Institute of Fisheries Technology, 682 029, Cochin, India

    D. Muthuchelvan

  3. Project Directorate on Foot-and-Mouth Disease, IVRI Campus Mukteswar-Kumaon, 263 138, Nainital, India

    A. Sanyal

  4. Indian Veterinary Research Institute, Mukteswar Campus, 263 138, Nainital, India

    R. Kaul & R. K. Singh

  5. Animal Husbandry Commission, 234A, Krishi Bhavan, 110 001, New Delhi, India

    S. K. Bandyopadhyay

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  1. P. Dhar
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  2. D. Muthuchelvan
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  4. R. Kaul
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  5. R. P. Singh
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  6. R. K. Singh
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  7. S. K. Bandyopadhyay
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Correspondence to S. K. Bandyopadhyay.

Additional information

*The sequence data reported in this paper have been submitted to the GenBank and have been assigned the Accession Number: AY560591.

P. Dhar and D. Muthuchelvan equally contributed to the work.

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Dhar, P., Muthuchelvan, D., Sanyal, A. et al. Sequence Analysis of the Haemagglutinin and Fusion Protein Genes of Peste-des-petits Ruminants Vaccine Virus of Indian Origin. Virus Genes 32, 71–78 (2006). https://doi.org/10.1007/s11262-005-5847-y

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  • DOI: https://doi.org/10.1007/s11262-005-5847-y

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