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Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus

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Abstract

Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) odv-e25 is 684 bp long, potentially encoding 227 amino acids with a predicted molecular weight of 24.9 kDa. Homology analysis indicated that SpltMNPV ODV-E25 has 35–65% amino acid identity with that of other known baculoviruses. RT-PCR results revealed that the odv-e25 is transcribed actively at the late stage of infection and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (TTAAG). Western blot analysis of odv-e25 expression with an antiserum made against 6 × His tagged ODV-E25 expressed in Escherichia coli indicated that it was present as a doublet of approximately 27 kDa from 24 h through 96 h in SpltMNPV-infected Spli-221 cells. Similar results were seen on Western blots of Spodoptera exigua (Se)MNPV-infected Se301 cells. Immunofluorescence analysis showed that ODV-E25 was predominantly present in the cytoplasm of SpltMNPV-infected cells and localized to the envelopes of occlusion-derived virus.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 30370965) and Guangdong Natural Science Foundation.

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Authors and Affiliations

  1. State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, P.R. China

    Zhaofei Li, Lijin Pan, Hang Yu, Sainan Li, Guoping Zhang & Yi Pang

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  1. Zhaofei Li
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  2. Lijin Pan
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  3. Hang Yu
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  6. Yi Pang
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Correspondence to Yi Pang.

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Li, Z., Pan, L., Yu, H. et al. Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus. Virus Genes 32, 13–19 (2006). https://doi.org/10.1007/s11262-005-5840-5

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  • DOI: https://doi.org/10.1007/s11262-005-5840-5

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