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A Moloney Murine Leukemia Virus Driven by the Jaagsiekte Sheep Retrovirus Enhancers Shows Enhanced Specificity for Infectivity in Lung Epithelial Cells

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Abstract

Jaagsiekte sheep retrovirus (JSRV) is the etiologic agent of ovine pulmonary adenocarcinoma (OPA), a transmissible lung cancer in sheep. One of the unique features of this virus is that in infected animals, the only tissues that show expression of the virus are the tumor cells in the lung. We previously showed that the JSRV long terminal repeat (LTR) is preferentially active in murine lung epithelial cell lines (MLE-15 and mtCC1-2). To further explore the tissue specificity, we inserted the JSRV enhancer sequences from the U3 region of the LTR into a Moloney murine leukemia virus (M-MuLV) LTR lacking its own enhancer sequences, to give the chimeric LTR ΔMo + JS. Transient transfection assays indicated that the ΔMo + JS LTR is > 5-fold more active in lung epithelial cell lines than in non-lung lines, compared to the wild-type M-MuLV LTR. This was due to preferential activity of the JSRV enhancers in lung epithelial cells. Moreover, M-MuLV driven by the ΔMo + JS LTR was > 3 logs more infectious in MLE-15 cells compared to non-lung cell lines. This chimeric virus may facilitate investigations of the tissue-specificity of JSRV.

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Authors and Affiliations

  1. Department of Molecular Biology and Biochemistry and Cancer Research Institute, University of California, Irvine, CA, 92697-3905, USA

    Kathleen Mcgee-Estrada & Hung Fan

  2. Departrnent of Veterinary Microbiology, University of Georgia, Athens, GA, USA

    Massimo Palmarini

  3. Department of Virology, Faculty of Health Sciences, University of KwaZulu-Natal, Durban, South Africa

    Claus Hallwirth

Authors
  1. Kathleen Mcgee-Estrada
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  2. Massimo Palmarini
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  3. Claus Hallwirth
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  4. Hung Fan
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Correspondence to Hung Fan.

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Mcgee-Estrada, K., Palmarini, M., Hallwirth, C. et al. A Moloney Murine Leukemia Virus Driven by the Jaagsiekte Sheep Retrovirus Enhancers Shows Enhanced Specificity for Infectivity in Lung Epithelial Cells. Virus Genes 31, 257–263 (2005). https://doi.org/10.1007/s11262-005-3239-y

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  • DOI: https://doi.org/10.1007/s11262-005-3239-y

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