Skip to main content

Advertisement

SpringerLink
Log in

Apoptin Induces Chromatin Condensation in Normal Cells

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Apoptin, a chicken anemia virus protein, was reported to induce tumor specific apoptosis, which was correlated with the nuclear localization of the protein in tumor cells. While in normal human cells, Apoptin was detected mainly in the cytoplasm and did not induce apoptosis. Using a recombinant adenovirus expressing Apoptin, we have found that Apoptin induced G2-M cell cycle arrest and chromatin condensation in cancer cells. Here we report that adenovirus mediated Apoptin expression also induces G2-M arrest in normal cells. In normal cells Apoptin is localized mainly in the cytoplasm but is also found in the nucleus of a subset of cells. Apoptin induces chromatin condensation not only when it is expressed in the nucleus but also when it is expressed in the cytoplasm. Our results indicate that Apoptin-induced chromatin condensation in the normal cells may not correlate with its nuclear localization and the mechanism of regulating the G2-M transition might be a target for Apoptin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M.H.M. Noteborn G. Koch (1995) Avian Pathol 24 11–31

    Google Scholar 

  2. A.A. Danen-Van Oorschot D.F. Fischer J.M. Grimbergen B. Klein S. Zhuang J.H. Falkenburg C. Backendorf P.H. Quax A.J. Van der Eb M.H. Noteborn (1997) Proc Natl Acad Sci USA 94 5843–5847 Occurrence Handle1:CAS:528:DyaK2sXjsFOitbg%3D Occurrence Handle9159162

    CAS  PubMed  Google Scholar 

  3. X. He S. Shang Y. Liu Q. Zhang S. Wang (2004) Beijing Da Xue Xue Bao 36 263–267 Occurrence Handle1:CAS:528:DC%2BD2cXhtVCis7zE Occurrence Handle15205696

    CAS  PubMed  Google Scholar 

  4. S.A. Azizi D. Stokes B.J. Augelli C. DiGirolamo D.J. Prockop (1998) Proc Natl Acad Sci USA 95 3908–3913 Occurrence Handle10.1073/pnas.95.7.3908 Occurrence Handle1:CAS:528:DyaK1cXitlKjuro%3D Occurrence Handle9520466

    Article  CAS  PubMed  Google Scholar 

  5. T.C. He S. Zhou L.T. da Costa J. Yu K.W. Kinzler B. Vogelstein (1998) Proc Natl Acad Sci USA 95 2509–2514 Occurrence Handle10.1073/pnas.95.5.2509 Occurrence Handle1:CAS:528:DyaK1cXhslehsLw%3D Occurrence Handle9482916

    Article  CAS  PubMed  Google Scholar 

  6. Precious B. and Russell W.C. in Mahy B.W.J. (ed.), Virology – A Practical Approach. IRL Press, Washington, 1985, pp. 193–205.

  7. A.A. Danen-Van Oorschot Y.H. Zhang S.R. Leliveld J.L. Rohn M.C. Seelen M.W. Bolk A. Van Zon S.J. Erkeland J.P. Abrahams D. Mumberg M.H. Noteborn (2003) J Biol Chem 278 27729–27736 Occurrence Handle1:CAS:528:DC%2BD3sXlsFKktLs%3D Occurrence Handle12754198

    CAS  PubMed  Google Scholar 

  8. M.H. Noteborn Y.H. Zhang A.J. van der Eb (1998) Mutat Res 400 447–455 Occurrence Handle1:CAS:528:DyaK1cXks1Onsb4%3D Occurrence Handle9685703

    CAS  PubMed  Google Scholar 

  9. Y.H. Zhang P.J. Abrahams A.J. van der Eb M.H. Noteborn (1999) Cancer Res 59 3010–3015 Occurrence Handle1:CAS:528:DyaK1MXktVyju7w%3D Occurrence Handle10383168

    CAS  PubMed  Google Scholar 

  10. J.L. Rohn Y.H. Zhang R.I. Aalbers N. Otto J. Den Hertog N.V. Henriquez C.J. Van De Velde P.J. Kuppen D. Mumberg P. Donner M.H. Notebom (2002) J Biol Chem 277 50820–50827 Occurrence Handle1:CAS:528:DC%2BD38Xps12ntbw%3D Occurrence Handle12393903

    CAS  PubMed  Google Scholar 

  11. J.S. Wadia M.V. Wagner S.A. Ezhevsky S.F. Dowdy (2004) J Virol 78 6077–6078 Occurrence Handle10.1128/JVI.78.11.6077-6078.2004 Occurrence Handle1:CAS:528:DC%2BD2cXksFCgtLg%3D Occurrence Handle15141008

    Article  CAS  PubMed  Google Scholar 

  12. L. Guelen H. Paterson J. Gaken M. Meyers F. Farzaneh M. Tavassoli (2004) Oncogene 23 1153–1165 Occurrence Handle10.1038/sj.onc.1207224 Occurrence Handle1:CAS:528:DC%2BD2cXpsFSrug%3D%3D Occurrence Handle14691460

    Article  CAS  PubMed  Google Scholar 

  13. S.M. Zhuang A. Shvarts H. van Ormondt A.G. Jochemsen A.J. van der Eb M.H. Noteborn (1995) Cancer Res 55 486–489 Occurrence Handle1:CAS:528:DyaK2MXjtlyktro%3D Occurrence Handle7834613

    CAS  PubMed  Google Scholar 

  14. A.A. Danen-Van Oorschot Y. Zhang S.J. Erkeland D.F. Fischer A.J. van der Eb M.H. Noteborn (1999) Leukemia 13 IssueIDSuppl 1 S75–77 Occurrence Handle10232370

    PubMed  Google Scholar 

  15. A.A. Danen-Van Oorschot A.J. van der Eb M.H. Noteborn (1999) Adv Exp Med Biol 457 245–249 Occurrence Handle1:STN:280:DyaK1MvjtVGrtA%3D%3D Occurrence Handle10500799

    CAS  PubMed  Google Scholar 

  16. A.A. Danen-van Oorschot A J. van Der Eb M.H. Noteborn (2000) J Virol 74 7072–7078 Occurrence Handle1:CAS:528:DC%2BD3cXkvFCmtbY%3D Occurrence Handle10888647

    CAS  PubMed  Google Scholar 

  17. M. Belyavskyi S.C. Braunagel M.D. Summers (1998) Proc Natl Acad Sci USA 95 11205–11210 Occurrence Handle10.1073/pnas.95.19.11205 Occurrence Handle1:CAS:528:DyaK1cXmt1ahtLY%3D Occurrence Handle9736714

    Article  CAS  PubMed  Google Scholar 

  18. E. Morita K. Tada H. Chisaka H. Asao H. Sato N. Yaegashi K. Sugamura (2001) J Virol 75 7555–7563 Occurrence Handle10.1128/JVI.75.16.7555-7563.2001 Occurrence Handle1:CAS:528:DC%2BD3MXlsFKku7g%3D Occurrence Handle11462027

    Article  CAS  PubMed  Google Scholar 

  19. G.J. Poggioli T.S. Dermody K.L. Tyler (2001) J Virol 75 7429–7434 Occurrence Handle10.1128/JVI.75.16.7429-7434.2001 Occurrence Handle1:CAS:528:DC%2BD3MXlsFKkuro%3D Occurrence Handle11462015

    Article  CAS  PubMed  Google Scholar 

  20. F.M. Jault J.M. Jault F. Ruchti E.A. Fortunato C. Clark J. Corbeil D.D. Richman D.H. Spector (1995) J Virol 69 6697–6704 Occurrence Handle1:CAS:528:DyaK2MXoslWktrc%3D Occurrence Handle7474079

    CAS  PubMed  Google Scholar 

  21. J. He S. Choe R. Walker P. Di Marzio D.O. Morgan N.R. Landau (1995) J Virol 69 6705–6711 Occurrence Handle1:CAS:528:DyaK2MXoslWkt74%3D Occurrence Handle7474080

    CAS  PubMed  Google Scholar 

  22. J.G. Teodoro D.W. Heilman A.E. Parker M.R. Green (2004) Genes Dev 18 1952–1957 Occurrence Handle10.1101/gad.1198404 Occurrence Handle1:CAS:528:DC%2BD2cXmvFKqsLc%3D Occurrence Handle15314021

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central Laboratory, Peking University People’s Hospital, 100044, Beijing, China

    Xiangjun He, Qi Zhang, Yujing Liu & Peiying He

Authors
  1. Xiangjun He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yujing Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peiying He
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

He, X., Zhang, Q., Liu, Y. et al. Apoptin Induces Chromatin Condensation in Normal Cells. Virus Genes 31, 49–55 (2005). https://doi.org/10.1007/s11262-005-2200-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-005-2200-4

Keywords

Search

Navigation