Skip to main content

Advertisement

SpringerLink
Log in

Detection of unusual mutation within the VP1 region of different re-isolates of poliovirus Sabin vaccine

  • Original Article
  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

In the present study, a genomic analysis of full VP1 sequence region of 15 clinical re-isolates (14 healthy vaccinees and one bone marrow tumor patient) was conducted, aiming to the identification of mutations and to the assessment of their impact on virus fitness, providing also insights relevant with the natural evolution of Sabin strains. Clinical re-isolates were analyzed by RT-PCR, sequencing and computational analysis. Some re-isolates were characterized by an unusual mutational pattern in which non-synonymous mutations outnumbered the synonymous ones. Furthermore, the majority of amino-acid substitutions were located in the capsid exterior, specifically in N-Ags, near N-Ags and in the north rim of the canyon. Also mutations, which are well-known determinants of attenuation, were identified. The results of this study propose that some re-isolates are characterized by an evolutionary pattern in which non-synonymous mutations with a direct phenotypic impact on viral fitness are fixed in viral genomes, in spite of synonymous ones with no phenotypic impact on viral fitness. Results of the present retrospective characterization of Sabin clinical re-isolates, based on the full VP1 sequence, suggest that vaccine-derived viruses may make their way through narrow breaches and may evolve into transmissible pathogens even in adequately immunized populations. For this reason increased poliovirus laboratory surveillance should be permanent and full VP1 sequence analysis should be conducted even in isolates originating from healthy vaccinees.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. E. Wimmer, C.U. Hellen, X. Cao, Annu. Rev. Genet. 27, 353–436 (1993)

    Article  CAS  Google Scholar 

  2. E.A. Emini, B.A. Jameson, A.J. Lewis, G.R. Larsen, E. Wimmer, J. Virol. 43(3), 997–1005 (1982)

    CAS  PubMed  PubMed Central  Google Scholar 

  3. J.M. Hogle, M. Chow, D.J. Filman, Science 229(4720), 1358–1365 (1985)

    Article  CAS  Google Scholar 

  4. P.D. Minor, M. Ferguson, D.M.A. Evans, J.W. Almond, J.P. Icenogle, J. Gen. Virol. 67(pt 7), 1283–1291 (1986)

    Article  CAS  Google Scholar 

  5. G. Bernhardt, J. Harber, A. Zibert, M. deCrombrugghe, E. Wimmer, Virology 203(2), 344–356 (1994)

    Article  CAS  Google Scholar 

  6. E. Colston, V.R. Racaniello, EMBO J. 13(24), 5855–5862 (1994)

    Article  CAS  Google Scholar 

  7. J. Harber, G. Bernhardt, H.H. Lu, J.Y. Sgro, E. Wimmer, Virology 214(2), 559–570 (1995)

    Article  CAS  Google Scholar 

  8. S. Liao, V.R. Racaniello, J. Virol. 71(12), 9770–9777 (1997)

    CAS  PubMed  PubMed Central  Google Scholar 

  9. D.J. Filman, R. Syed, M. Chow, A.J. Macadam, J.M. Hogle, EMBO J. 8(5), 1567–1579 (1989)

    Article  CAS  Google Scholar 

  10. K. Kirkegaard, J. Virol. 64(1), 195–206 (1990)

    CAS  PubMed  PubMed Central  Google Scholar 

  11. C.E. Fricks, J.M. Hogle, J. Virol. 64(5), 1934–1945 (1990)

    CAS  PubMed  PubMed Central  Google Scholar 

  12. M.J. Bouchard, D.H. Lam, V.R. Racaniello, J. Virol. 69(8), 4972–4978 (1995)

    CAS  PubMed  PubMed Central  Google Scholar 

  13. A.J. Macadam, S.R. Pollard, G. Ferguson, R. Skuce, D. Wood, J.W. Almond, Virology 192, 18–26 (1993)

    Article  CAS  Google Scholar 

  14. A.J. Macadam, C. Arnold, J. Howlett, A. John, S. Marsden, F. Taffs, P. Reeve, N. Hamada, K. Wareham, J. Almond, N. Cammack, P.D. Minor, Virology 172, 408–414 (1989)

    Article  CAS  Google Scholar 

  15. M.G. Rossmann, E. Arnold, J.W. Erickson, E.A. Frankenberger, J.P. Griffith, H.J. Hecht, J.E. Johnson, G. Kamer, M. Luo, A.G. Mosser, et al. Nature 317(6033), 145–153 (1985)

    Article  CAS  Google Scholar 

  16. E.M. Colston, V.R. Racaniello, J. Virol. 69(8), 4823–4829 (1995)

    CAS  PubMed  PubMed Central  Google Scholar 

  17. World Health Organization. Wkly Epidemiol. Rec. 78(17), 138–144 (2003)

    Google Scholar 

  18. W.R. Dowdle, E. De Gourville, O.M. Kew, M.A. Pallansch, D.J. Wood, Rev. Med. Virol. 13(5), 277–291 (2003)

    Article  Google Scholar 

  19. World Health Organization, Manual for the virological investigation of poliomyelitis. (World Health Organization, Geneva, Switzerland, 2004)

  20. A. Georgopoulou, P. Markoulatos, N. Spyrou, N.C. Vamvakopoulos, J. Clin. Microbiol. 38, 4337–4342 (2000)

    CAS  PubMed  PubMed Central  Google Scholar 

  21. I. Casas, L. Powell, P.E. Klapper, G.M. Cleator, J. Virol. Methods 53, 25–36 (1995)

    Article  CAS  Google Scholar 

  22. J. Balanant, S. Guillot, A. Candrea, F. Delpeyroux, R. Crainic, Virology 184, 645–654 (1991)

    Article  CAS  Google Scholar 

  23. M.S. Oberste, K. Maher, M.R. Flemister, G. Marchetti, D.R. Kilpatrick, M.A. Pallansch, J. Clin. Microbiol. 38, 1170–1174 (2000)

    CAS  PubMed  PubMed Central  Google Scholar 

  24. M.W. Wien, S. Curry, D.J. Filman, J.M. Hogle, Nat. Struct. Biol. 4, 666–674 (1997)

    Article  CAS  Google Scholar 

  25. R.A. Grant, C.N. Hiremath, D.J. Filman, R. Syed, K. Andries, J.M. Hogle, Curr. Biol. 4, 784–797 (1994)

    Article  CAS  Google Scholar 

  26. Y. He, V.D. Bowman, S. Mueller, C.M. Bator, J. Bella, X. Peng, T.S. Baker, E. Wimmer, R.J. Kuhn, M.G. Rossmann, Proc. Natl. Acad. Sci. USA 97, 79–84 (2000)

    Article  CAS  Google Scholar 

  27. E.A. Cherkasova, E.A. Korotkova, M.L. Yakovenko, O.E. Ivanova, T.P. Eremeeva, K.M. Chumakov, V.I. Agol, J. Virol. 76, 6791–6799 (2002)

    Article  CAS  Google Scholar 

  28. A. Belhmunt, G. May, R. Zell, P. Pring-Akerblom, W. Verhagen, A. Heim, Virology 265, 178–184 (1999)

    Article  Google Scholar 

  29. M.M. Georgescu, J. Balanant, A. Macadam, D. Otelea, M. Combiescu, A.A. Combiescu, R. Crainic, F. Delpeyroux, J. Virol. 71, 7758–7768 (1997)

    CAS  PubMed  PubMed Central  Google Scholar 

  30. O.M. Kew, R.W. Sutter, B.K. Nottay, M.J. McDonugh, D.R. Prevots, L. Quick, M.A. Pallansch, J. Clin. Microbiol. 36, 2893–2899 (1998)

    CAS  PubMed  PubMed Central  Google Scholar 

  31. G.V. Gavrilin, E.A. Cherkasova, G.Y. Lipskaya, O.M. Kew, V.I. Agol, J. Virol. 7, 7381–7390 (2000)

    Article  Google Scholar 

  32. P.E. Lobert, N. Escriou, J. Ruelle, T. Michiels, Proc. Natl. Acad. Sci. USA 96, 11560–11565 (1999)

    Article  CAS  Google Scholar 

  33. K.L. McKnight, S.M. Lemon, J. Virol. 70, 1941–1952 (1996)

    CAS  PubMed  PubMed Central  Google Scholar 

  34. M.N. Mulders, J.H. Reimerink, M. Stenvik, I. Alaeddinoglu, H.G. van der Avoort, T. Hovi, M.P. Koopmans, J. Gen. Virol. 80, 907–916 (1999)

    Article  CAS  Google Scholar 

  35. L.M. Tatem, C. Weeks-Levy, A. Georgiou, S.J. Dimichelle, E.J. Gorgacz, V.R. Racaniello, F.R. Cano, S.J. Mento, J. Virol. 66, 3194–3197 (1992)

    CAS  PubMed  PubMed Central  Google Scholar 

  36. D.M. Belnap, B.M. Jr. McDermott, D.J. Filman, N. Cheng, B.L. Trus, H.J. Zuccola, V.R. Racaniello, J.M. Hogle, A.C. Steven, Proc. Natl. Acad. Sci. USA 97, 73–78 (2000)

    Article  CAS  Google Scholar 

  37. Centers for Disease Control and Prevention (CDC), MMWR Morb. Mortal. Wkly. Rep. 50, 41–42, 51 (2001)

    Google Scholar 

  38. Centers for Disease Control and Prevention (CDC), MMWR Morb. Mortal. Wkly. Rep. 50, 874–875 (2001)

    Google Scholar 

  39. Centers for Disease Control and Prevention (CDC), MMWR Morb. Mortal. Wkly. Rep. 50, 855–856 (2001)

    Google Scholar 

  40. L.M. Shulman, Y. Manor, R. Handsher, F. Delpeyroux, M.J. McDonough, T. Halmut, I. Silberstein, J. Alfandari, J. Quay, T. Fisher, J. Robinov, O.M. Kew, R. Crainic, E. Mendelson, J. Clin. Microbiol. 38, 3729–3374 (2000)

    CAS  PubMed  PubMed Central  Google Scholar 

  41. I. Karakasiliotis, E. Paximadi, P. Markoulatos, J. Gen. Virol. 86, 3137–3142 (2005)

    Article  CAS  Google Scholar 

  42. C.F. Yang, H.Y. Chen, J. Jorba, H.C. Sun, S.J. Yang, H.C. Lee, Y.C. Huang, T.Y. Lin, P.J. Chen, H. Shimizu, Y. Nishimura, A. Utama, M. Pallansch, T. Miyamura, O. Kew, J.Y. Yang, J. Virol. 79, 12623–12634 (2005)

    Article  CAS  Google Scholar 

  43. B. Cernakova, Z. Sobotova, I. Rovny, S. Blahova, M. Roivainen, T. Hovi, Eur. J. Clin. Microbiol. Infect. Dis. 24, 438–439 (2005)

    Article  CAS  Google Scholar 

Download references

Acknowledgement

The present work was co funded by the European Union (75%) and the Greek Ministry of Education (25%) under the framework of the Education and Initial Vocational Training Program “Pythagoras II”. Research project: “Molecular detection of Enteroviruses in clinical samples and the environment. Implications for the public health”. Code 52213.11.

Author information

Authors and Affiliations

  1. School of Health Sciences, Department of Biochemistry & Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, 41221, Larissa, Greece

    Evaggelos Dedepsidis, Eleni Paximadi, Zaharoula Kyriakopoulou, Dimitrios Komiotis & Panayotis Markoulatos

  2. School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading, UK

    Ioannis Karakasiliotis

Authors
  1. Evaggelos Dedepsidis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ioannis Karakasiliotis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eleni Paximadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zaharoula Kyriakopoulou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dimitrios Komiotis
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Panayotis Markoulatos
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Panayotis Markoulatos.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dedepsidis, E., Karakasiliotis, I., Paximadi, E. et al. Detection of unusual mutation within the VP1 region of different re-isolates of poliovirus Sabin vaccine. Virus Genes 33, 183–191 (2006). https://doi.org/10.1007/s11262-005-0055-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-005-0055-3

Keywords

Search

Navigation