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Genetic Variability of Hepatitis B Virus Isolates in Poland

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Abstract

There is very limited knowledge about the genetic variability of HBV strains circulating in the population of Polish chronically infected HBV patients. The aim of this study was to analyse the phylogenetic relatedness and polymorphism in some functional domains of HBV genome among chronically infected patients from northern Poland. Fifty-one serum samples were included to analysis of HBV genomes due to the viral load sufficient for DNA preparation and sequencing. The sequences of the rt polymerase/S and preC/BCP regions of those isolates were analysed, compared to genome sequences of different variants of HBV from GenBank database and genetic relatedness of Polish genotypes to known reference strains was estimated. A phylogenetic tree of 41 analysed genotype A isolates as well as 8 genotype D strains was constructed showing relationship to know reference strains. Two isolates, initially classified as genotype F turned to be related to genotype H, newly described genotype deriving from genotype F, a very rare genotype in Europe. HBV genotypes’ distribution pattern in Poland and phylogenetic relatedness seems to be different from our Eastern neighbours. Due to the fact that Poland is still ethnically uniform country, it is interesting to explore molecular epidemiology of HBV infections in our population.

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Abbreviations

BCP:

Basal core promoter

bp:

Base pairs

HBV:

Hepatitis B

HBx:

X gene

ORF:

Open reading frame

preC:

Precore

SD:

Standard deviation

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Acknowledgements

The authors thank to Prof Fabien Zoulim, Dr. Helene Norder and Dr Marisol Devesa for fruitful discussion in Lyon and inspiration for the phylogenetic analysis of HBV genotypes from Poland. This study was supported by the Fifth Framework Programme (QRLT 2000–00977) and is also part of the activities of the VIRGIL European Network of Excellence on Antiviral Drug Resistance supported by a grant (LSHM-CT-2004-503359) from the Priority 1 “Life Sciences, Genomics and Biotechnology for Health” programme in the 6th Framework Programme of the EU.

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Authors and Affiliations

  1. Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk, ul. Kladki 24, 80-822, Gdansk, Poland

    Krzysztof Piotr Bielawski, Urszula Charmuszko, Aleksandra Dybikowska & Anna Jadwiga Podhajska

  2. Department of Infectious Diseases, Medical University of Gdansk, Smoluchowskiego 18, 80-214, Gdansk, Poland

    Piotr Stalke & Anna Jadwiga Podhajska

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  1. Krzysztof Piotr Bielawski
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  2. Urszula Charmuszko
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  3. Aleksandra Dybikowska
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  4. Piotr Stalke
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  5. Anna Jadwiga Podhajska
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Correspondence to Krzysztof Piotr Bielawski.

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Bielawski, K.P., Charmuszko, U., Dybikowska, A. et al. Genetic Variability of Hepatitis B Virus Isolates in Poland. Virus Genes 33, 77–86 (2006). https://doi.org/10.1007/s11262-005-0040-x

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