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Virus Genes

, Volume 29, Issue 3, pp 303–309 | Cite as

Molecular Characterization of Two Distinct Begomovirusesfrom Papaya in China

  • Xiangyang Wang
  • Yan Xie
  • Xueping Zhou
Article

Abstract

Six papaya samples showing downward leaf curling were collected in Guangdong and Guangxi provinces, China. The result of TAS-ELISA showed they were all infected by geminiviruses. Comparison of partial DNA-A sequences reveals that these virus isolates can be classified into two groups. Group I includes isolates G2, G4, G5, G28 and G29 from Guangxi province, while isolate GD2 from Guangdong province belongs to Group II. The complete DNA-A sequence of G2 and GD2 were characterized. Sequence comparisons showed that the DNA-A of G2 and GD2 were most closely related to that of Ageratum yellow vein China virus-[Hn2] and Ageratum yellow vein virus, respectively, with 83.4 and 75.2% nucleotide sequence identity, while DNA-A sequence between G2 and GD2 had only 73.4% sequence identity. The molecular data suggests that G2 and GD2 are two distinct begomoviruses, for which the name Papaya leaf curl China virus (PaLCuCNV) for G2 and Papaya leaf curl Guangdong virus (PaLCuGDV) for GD2 are proposed. Comparison of individual encoded proteins showed the coat protein of G2 and GD2 shared highest amino acid sequence identity (97.7 and 94.2%, respectively) with that of Pepper leaf curl virus-[Malaysia] (PepLCV-[MY]), suggesting the CP of these viruses may have identical ancestor.

begomovirus DNA-A nucleotide sequence Papaya leaf curl virus 

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References

  1. 1.
    Fauquet C. M., Bisaro D. M., Briddon R. W., Brown J. K., Harrison B. D., Rybicki E. P., Stenger D. C., and Stanley J., Arch Virol 148, 405–421, 2003.PubMedGoogle Scholar
  2. 2.
    Rochester D. E., Kositratana W., and Beachy R. N., Virology 178, 520–526, 1990.PubMedGoogle Scholar
  3. 3.
    Saunders K., Bedford I. D., Briddon R. W., Markham P. G., Wong S. M., and Stanley J., Proc Natl Acad Sci USA 97, 6890–6895, 2000.PubMedGoogle Scholar
  4. 4.
    Jose J. and Usha R., Virology 305, 310–317, 2003.PubMedGoogle Scholar
  5. 5.
    Zhou X. P., Xie Y., Tao X. R., Zhang Z. K., Li Z. H., and Fauquet C. M., J Gen Virology 84, 237–247, 2003.Google Scholar
  6. 6.
    Moffat A. S., Science 286, 1835, 1999.Google Scholar
  7. 7.
    Zhou X. P., Peng Y., Xie Y., and Zhang Z. K., Chinese Sci Bull 48, 2205–2209, 2003.Google Scholar
  8. 8.
    Zhou X. P., Liu Y. L., Calvert L., Munoz C., Otim-Nape G. W., Robinson D. J., and Harrison B. D., J Gen Virol 78, 2101–2111, 1997.PubMedGoogle Scholar
  9. 9.
    Xie Y., Zhou X. P., Zhang Z. K., and Qi Y. J., Chinese Sci Bull 47, 197–200, 2002.Google Scholar
  10. 10.
    He G. A., Xiao H. G., Zhang S. G., Li H. P., and Fan H. Z., Acta Phytopathol Sinica 32(1), 21–25, 2002.Google Scholar
  11. 11.
    Harrison B. D. and Robinson D. J., Annu Rev Phytopathol 37, 369–398, 1999.PubMedGoogle Scholar
  12. 12.
    Yin Q. Y., Yang H. Y., Gong Q. H., Wang H. Y., Liu Y. L., Hong Y. G., and Tien P., Virus Res 81, 69–76, 2001.PubMedGoogle Scholar
  13. 13.
    Xie Y., Zhou X. P., Li Z. H., Zhang Z. K., and Li G. X., Chinese Sci Bull, 47, 1273–1276, 2002.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Xiangyang Wang
    • 1
  • Yan Xie
    • 1
  • Xueping Zhou
    • 1
  1. 1.Institute of BiotechnologyZhejiang UniversityHangzhouPeople's Republic of China

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