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Physical and Partial Genetic Map of Spodoptera frugiperda Nucleopolyhedrovirus (SfMNPV) Genome

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Abstract

A Nicaraguan isolate of Spodoptera frugiperda multicapsid nucleopolyhedrovirus (SfMNPV) is undergoing field trials for control of this pest in the Americas. This isolate is composed of multiple genotypes, some of which are deletion mutants. Identification of the genetic changes in deleted genotypes cannot be accomplished without the construction of a detailed physical map. In the present study, combinations of restriction endonuclease analysis and Southern blot analysis was performed. This map was refined by sequencing the termini of cloned restriction fragments. The SfMNPV genome was estimated to be 129.3 kb, 8 kb larger than the previously characterized Sf-2 variant from the United States, due to a deletion between 14.8 and 21.0 m.u. in the physical map described in this study. A total of 27.92 kb were sequenced, which represented 21.5% of the whole genome and included 38 ORFs. Comparison with other sequenced baculoviruses revealed that SfMNPV displayed the highest sequence identity (66%) and gene arrangement (78%) with Spodoptera exigua MNPV, sharing 36 putative ORFs. In addition, the genome organization was similar to that of SeMNPV, with minor differences. Phylogenetic analysis confirmed the close relatedness between SeMNPV and SfMNPV, suggesting they evolved from a common ancestor.

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References

  1. van Regenmortel M.H.V., Fauquet C.M., Bishop D.H.L., Carstens E.B., Estes M.K., Lemon S.M., Maniloff J., Mayo M.A., McGeoch D.J., Pringle C.R., and Wickner R.B. (eds.), Virus Taxonomy: The Classification and Nomenclature of Viruses. The Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego, 2000

  2. E.A. Herniou J.A. Olszewski J.S. Cory D.R. O’Reilly (2003) Annu Rev Entomol 48 211–234

    Google Scholar 

  3. T. Hayakawa G.F. Rohrmann Y. Hashimoto (2000) Virology 278 1–12

    Google Scholar 

  4. J.R. Fuxa (1991) Biotechnol Adv 9 425–442

    Google Scholar 

  5. F. Moscardi (1999) Ann Rev Entomol 44 257–289

    Google Scholar 

  6. M. López-Ferber W.P. Sisk R.D. Possee (1995) Methods Mol Biol 39 25–63

    Google Scholar 

  7. J.M. Fernandez J.P. Hoeffler (Eds) (1999) Gene Expression Systems: Using Nature for the Art of Expression Academic Press San Diego 332–359

    Google Scholar 

  8. M.D. Ayres S.C. Howard J. Kuzio M. López-Ferber R.D. Possee (1994) Virology 202 586–605

    Google Scholar 

  9. W.A. Gardner J.R. Fuxa (1980) Fla Entomol 63 439–447

    Google Scholar 

  10. A.N. Sparks (1979) Fla Entomol 62 82–87

    Google Scholar 

  11. D.I. Shapiro J.R. Fuxa H.D. Braymer D.P. Pashley (1991) J Invertebr Pathol 58 96–105

    Google Scholar 

  12. A. Escribano T. Williams D. Goulson R.D. Cave J.W. Chapman P. Caballero (1999) J Econ Entomol 92 1079–1085

    Google Scholar 

  13. J.E. Maruniak S.E. Brown D.L. Knudson (1984) Virology 136 221–234

    Google Scholar 

  14. J.D. Knell M.D. Summers (1981) Virology 112 190–197

    Google Scholar 

  15. L.C. Loh J.J. Hamm E.S. Huang (1981) J Virol 38 922–931

    Google Scholar 

  16. D.C. Kelly (1977) Virology 76 468–471

    Google Scholar 

  17. M.A. Gonzalez G.E. Smith M.D. Summers (1989) Virology 170 160–175

    Google Scholar 

  18. J.C. Liu J.E. Maruniak (1995) J Gen Virol 76 1443–1450

    Google Scholar 

  19. V.F. Tumilasci E. Leal P.M. Zanotto T. Luque J.L. Wolff (2003) Virus Genes 27 137–144

    Google Scholar 

  20. T. Williams D. Goulson P. Caballero J. Cisneros A.M. Martínez J.W. Chapman D.X. Roman R.D. Cave (1999) Biol Control 14 67–75

    Google Scholar 

  21. O. Simon T. Williams M. López-Ferber P. Caballero (2004) Appl Environ Microbiol 70 5579–5588

    Google Scholar 

  22. M. López-Ferber O. Simon T. Williams P. Caballero (2003) Proc R Soc Lond B Biol Sci 270 2249–2255

    Google Scholar 

  23. G.L. Greene N.C. Leppla W.A. Dickerson (1976) J Econ Entomol 69 487–488

    Google Scholar 

  24. P.R. Hughes H.A. Wood (1981) J Invertebr Pathol 37 154–159

    Google Scholar 

  25. P. Caballero D. Zuidema C. Santiago-Alvarez J.M. Vlak (1992) Biocontrol Sci Technol 2 145–157

    Google Scholar 

  26. G. Croizier C.P. Ribeiro (1992) Virus Res 26 183–196

    Google Scholar 

  27. T. Maniatis E.F. Fritsch J. Sambrook (1982) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY

    Google Scholar 

  28. W.R. Pearson (1990) Methods Enzymol 183 63–98 Occurrence Handle10.1016/0076-6879(90)83007-V Occurrence Handle1:CAS:528:DyaK3cXkslOhuro%3D Occurrence Handle2156132

    Article  CAS  PubMed  Google Scholar 

  29. S.F. Altschul W. Gish W. Miller E.W. Myers D.J. Lipman (1990) J Mol Biol 215 403–410 Occurrence Handle10.1006/jmbi.1990.9999 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D Occurrence Handle2231712

    Article  CAS  PubMed  Google Scholar 

  30. J.D. Thompson T.J. Gibson F. Plewniak F. Jeanmougin D.G. Higgins (1997) Nucleic Acids Res 24 4876–4882 Occurrence Handle10.1093/nar/25.24.4876

    Article  Google Scholar 

  31. J.M. Vlak G.E. Smith (1982) J Gen Virol 41 1118–1121

    Google Scholar 

  32. Felsenstein J., PHYLIP: Phylogenetics Inference Package, Version 3.5c., Seattle, Department of Genetics, University of Washington, 1993

  33. C.L. Afonso E.R. Tulman Z. Lu C.A. Balinsky B.A. Moser J.J. Becnel D.L. Rock G.F. Kutish (2001) J Virol 75 11157–11165

    Google Scholar 

  34. Chevenet F., TreeDyn: a dynamic graphics tree editor V1.62. http://viradium.mpl.ird.fr/treedyn, 2003

  35. I.R. Smith N.E. Crook (1988) Virology 166 240–244

    Google Scholar 

  36. W.F. Ijkel E.A. Strien Particlevan J.G. Heldens R. Broer D. Zuidema R.W. Goldbach J.M. Vlak (1999) J Gen Virol 80 3289–3304

    Google Scholar 

  37. A.M. Laitinen I.S. Otvos D.B. Lewis (1996) J Econ Entomol 89 640–647

    Google Scholar 

  38. Q. Li C. Donly L. Li L.G. Willis D.A. Theilmann M. Erlandson (2002) Virology 294 106–121

    Google Scholar 

  39. L. Li C. Donly Q. Li L.G. Willis B.A. Keddie M.A. Erlandson D.A. Theilmann (2002) Virology 297 226–244

    Google Scholar 

  40. X. Chen W.J. Zhang J. Wong G. Chun A. Lu B.F. McCutchen J.K. Presnail R. Herrmann M. Dolan S. Tingey Z.H. Hu J.M. Vlak (2002) J Gen Virol 83 673–684

    Google Scholar 

  41. X. Chen W.F.J. Ijkel R. Tarchini X. Sun H. Sandbrink H. Wang S. Peters D. Zuidema R.K. Lankhorst J.M. Vlak Z. Hu (2001) J Gen Virol 82 241–257

    Google Scholar 

  42. Y. Pang J. Yu L. Wang X. Hu W. Bao G. Li C. Chen H. Han S. Hu H. Yang (2001) Virology 287 391–404

    Google Scholar 

  43. J. Kuzio M.N. Pearson S.H. Harwood C.J. Funk J.T. Evans J.M. Slavicek G.F. Rohrmann (1999) Virology 253 17–34

    Google Scholar 

  44. S. Gomi K. Majima S. Maeda (1999) J Gen Virol 80 1323–1337

    Google Scholar 

  45. C.H. Ahrens R.L. Russell C.J. Funk J.T. Evans S.H. Harwood G.F. Rohrmann (1997) Virology 229 381–399

    Google Scholar 

  46. O. Hyink R.A. Dellow M.J. Olsen K.M. Caradoc-Davies K. Drake E.A. Herniou J.S. Cory D.R. O’Reilly V.K. Ward (2002) J Gen Virol 83 957–971

    Google Scholar 

  47. T. Hayakawa R. Ko K. Okano S.I. Seong C. Goto S. Maeda (1999) Virology 262 277–297

    Google Scholar 

  48. Y. Hashimoto T. Hayakawa Y. Ueno T. Fujita Y. Sano T. Matsumoto (2000) Virology 275 358–372

    Google Scholar 

  49. S. Wormleaton J. Kuzio D. Winstanley (2003) Virology 311 350–365

    Google Scholar 

  50. T. Luque R. Finch N. Crook D.R. O’Reilly D. Winstanley (2001) J Gen Virol 82 2531–2547

    Google Scholar 

  51. R.M.D. Page (2000) Mol Phylogenet Evol 14 89–106

    Google Scholar 

  52. J.A. Jehle (2004) Virus Genes 29 5–8

    Google Scholar 

  53. E.A. Herniou T. Luque X. Chen J.M. Vlak D. Winstanley J.S. Cory D.R. O’Reilly (2001) J Virol 75 8117–8126

    Google Scholar 

  54. Balachowsky A.S., (ed.), Famille des Noctuidae. Masson et Cie, Paris, 1972, pp. 1255–1520

  55. O. Simon T. Williams M. López-Ferber P. Caballero (2004) J Gen Virol 85 2845–2855

    Google Scholar 

  56. J.G. Heldens Y. Liu D. Zuidema R.W. Goldbach J.M. Vlak (1998) Virus Res 55 187–198

    Google Scholar 

  57. M. Kool J.T. Voeten R.W. Goldbach J.M. Vlak (1994) Virology 198 680–689

    Google Scholar 

Download references

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Correspondence to Miguel López-Ferber.

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Simón, O., Chevenet, F., Williams, T. et al. Physical and Partial Genetic Map of Spodoptera frugiperda Nucleopolyhedrovirus (SfMNPV) Genome. Virus Genes 30, 403–417 (2005). https://doi.org/10.1007/s11262-004-6784-x

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  • DOI: https://doi.org/10.1007/s11262-004-6784-x

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