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Genome Sequences and Structures of Two Biologically Distinct Strains of Grapevine leafroll-associated virus 2 and Sequence Analysis

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Grapevine leafroll-associated virus 2 (GLRaV-2), a member of the genus Closterovirus within Closteroviridae, is implicated in several important diseases of grapevines including “leafroll”, “graft-incompatibility”, and “quick decline” worldwide. Several GLRaV-2 isolates have been detected from different grapevine genotypes. However, the genomes of these isolates were not sequenced or only partially sequenced. Consequently, the relationship of these viral isolates at the molecular level has not been determined. Here, we group the various GLRaV-2 isolates into four strains based on their coat protein gene sequences. We show that isolates “PN” (originated from Vitis vinifera cv. “Pinot noir”), “Sem” (from V. vinifera cv. “Semillon”) and “94/970” (from V. vinifera cv. “Muscat of Alexandria”) belong to the same strain, “93/955” (from hybrid “LN-33”) and “H4” (from V. rupestris “St. George”) each represents a distinct strain, while Grapevine rootstock stem lesion-associated virus.

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  1. Martelli G.P., Saldarelli P., and Boscia D., in Monette P.L. (ed.), Filamentous Viruses of Woody Plants. Research Signpost, Trivandrum, India, 1997, pp. 1–9.

  2. Goheen A.C., in Pearson R.C. and Goheen A.C. (eds), Compendium of Grape Diseases. American Phytopathological Society Press, St. Paul, Minnesota, USA, 1988, pp 52.

  3. D. Boscia C. Greif P. Gugerli G.P. Martelli B. Walter D. Gonsalves (1995) Vitis 34 171–175

    Google Scholar 

  4. Martelli G.P., Extended Abstracts of 14th Meeting of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine, Bari, Italy, 2003, pp. 3–10.

  5. G.P. Martelli A.A. Agranovsky M. Bar-Joseph D. Boscia T. Candresse R.H.A. Coutts V.V. Dolia B.W. Falk D. Gonsalves W. Jelkmann A.V. Karasev A. Minafra S. Namba H.J. Vetten G.C. Wisler N. Yoshikawa (2002) Arch Virol 147 2039–2044 Occurrence Handle10.1007/s007050200048 Occurrence Handle1:CAS:528:DC%2BD38Xnslaqtb4%3D Occurrence Handle12376765

    Article  CAS  PubMed  Google Scholar 

  6. A.A. Agranovsky E.V. Koonin V.P. Boyko E. Maiss R. Frotschl N.A. Lunina J.G. Atabekov (1994) Virology 198 311–324 Occurrence Handle10.1006/viro.1994.1034 Occurrence Handle1:CAS:528:DyaK2cXpslKgsQ%3D%3D Occurrence Handle8259666

    Article  CAS  PubMed  Google Scholar 

  7. A.V. Karasev V.P. Boyko S. Gowda O.V. Nikolaeva M.E. Hilf E.V. Koonin C.L. Niblett K. Cline D.J. Gumpf R.F. Lee S.M. Garnsey D.J. Lewandowski W.O. Dawson (1995) J Virol 208 511–520 Occurrence Handle10.1006/viro.1995.1182 Occurrence Handle1:CAS:528:DyaK2MXlsF2ht7s%3D

    Article  CAS  Google Scholar 

  8. H.Y. Zhu K.S. Ling D.E. Goszczynski D. Gonsalves (1998) J Gen Virol 79 1289–1298 Occurrence Handle1:CAS:528:DyaK1cXivFOluro%3D Occurrence Handle9603345

    CAS  PubMed  Google Scholar 

  9. N. Abou-Ghanem S. Sabanadzovic A. Minafra P. Saldarelli G.P. Martelli (1998) J Plant Pahol 80 37–46 Occurrence Handle1:CAS:528:DyaK1cXkvVWmu7s%3D

    CAS  Google Scholar 

  10. C. Greif R. Garau D. Boscia V.A. Prota M. Fiori P. Bass B. Walter U. Prota (1995) Phytopathol Medit 34 167–173

    Google Scholar 

  11. J. Monis R.K. Bestwick (1997) Plant Dis 81 802–808

    Google Scholar 

  12. R. Bonfiglioli F. Edwards A. Pantaleo (2003) Aust NZ Grapegrower Winemaker 476 50–54

    Google Scholar 

  13. J.K. Uyemoto A. Rowhani D. Luvisi C.R. Krag (2001) Calif Agric 55 28–31

    Google Scholar 

  14. Rowhani A., Zhang Y.-P., Golino D.A.,and Uyemoto J.K., 2002. Phytopathology 92 S71.

  15. D.E. Goszczynski G.G.F. Kasdorf G. Pietersen H. Tonder Particlevan (1996) Vitis 35 133–135

    Google Scholar 

  16. K.-S. Ling H.Y. Zhu R.F. Drong J.L. Slightom J.R. McFerson D. Gonsalves (1998) J Gen Virol 79 1299–1307 Occurrence Handle1:CAS:528:DyaK1cXivFOlurs%3D Occurrence Handle9603346

    CAS  PubMed  Google Scholar 

  17. K.-S. Ling H.Y. Zhu D. Gonsalves (2004) J Gen Virol 85 2099–2102 Occurrence Handle10.1099/vir.0.80007-0 Occurrence Handle1:CAS:528:DC%2BD2cXlvFKhurs%3D Occurrence Handle15218195

    Article  CAS  PubMed  Google Scholar 

  18. N. Abou-Ghanem S. Sabanadzovic M.A. Castellano D. Boscia G.P. Martelli (2000) Vitis 39 119–121

    Google Scholar 

  19. J.S. Hu D. Gonsalves D. Teliz (1990) J Phytopathol 128 1–14

    Google Scholar 

  20. M.J. Adams J.F. Antoniw M. Bar-Joseph A.A. Brunt T. Candresse G.D. Foster G.P. Martelli R.G. Milne C.M. Fauquet (2004) Arch Virol 149 1045–1060 Occurrence Handle1:CAS:528:DC%2BD2cXjt1Sgtr4%3D Occurrence Handle15098118

    CAS  PubMed  Google Scholar 

  21. M. Mawassi E. Mietkiewska R. Gofman G. Yang M. Bar-Joseph (1996) J Gen Virol 77 2359–2364 Occurrence Handle1:CAS:528:DyaK28XltlKktr4%3D Occurrence Handle8811037

    CAS  PubMed  Google Scholar 

  22. A. Karasev (2000) Ann Rev Phytopathol 38 293–324 Occurrence Handle10.1146/annurev.phyto.38.1.293 Occurrence Handle1:CAS:528:DC%2BD3cXot1Sju74%3D

    Article  CAS  Google Scholar 

  23. C.-W. Peng V.V. Peremyslov A.R. Mushegian W.O. Dawson V.V. Dolja (2001) J Virol 75 12153–12160 Occurrence Handle10.1128/JVI.75.24.12153-12160.2001 Occurrence Handle1:CAS:528:DC%2BD3MXovVygtrY%3D Occurrence Handle11711606

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Baozhong Meng.

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Meng, B., Li, C., Goszczynski, D.E. et al. Genome Sequences and Structures of Two Biologically Distinct Strains of Grapevine leafroll-associated virus 2 and Sequence Analysis. Virus Genes 31, 31–41 (2005).

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